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Protein

Sodium/hydrogen exchanger 9B2

Gene

Slc9b2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Na+/H+ antiporter that extrudes Na+ or Li+ in exchange for external protons across the membrane (PubMed:18508966, PubMed:17988971). Contributes to the regulation of intracellular pH, sodium homeostasis, and cell volume. Plays an important role for insulin secretion and clathrin-mediated endocytosis in beta-cells (PubMed:23720317). Involved in sperm motility and fertility (PubMed:27010853). It is controversial whether SLC9B2 plays a role in osteoclast differentiation (PubMed:17988971) or not (PubMed:20441802, PubMed:22985540).6 Publications

Miscellaneous

Inhibited by phloretin but not by the classical SLC9A-inhibitor amiloride.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei278Important for cation transportBy similarity1
Sitei279Important for cation transportBy similarity1

GO - Molecular functioni

GO - Biological processi

  • clathrin-dependent endocytosis Source: UniProtKB
  • flagellated sperm motility Source: UniProtKB
  • positive regulation of osteoclast development Source: MGI
  • regulation of insulin secretion involved in cellular response to glucose stimulus Source: UniProtKB
  • sodium ion transport Source: MGI

Keywordsi

Biological processAntiport, Hydrogen ion transport, Ion transport, Sodium transport, Transport
LigandSodium

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/hydrogen exchanger 9B2
Alternative name(s):
NHA-oc1 Publication
Na(+)/H(+) exchanger NHA2
Na(+)/H(+) exchanger-like domain-containing protein 2
Short name:
NHE domain-containing protein 2
Soditlum/hydrogen exchanger-like domain-containing protein 2
Solute carrier family 9 subfamily B member 2
Gene namesi
Name:Slc9b2Imported
Synonyms:Nha2, Nhedc2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2140077. Slc9b2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei87 – 107HelicalSequence analysisAdd BLAST21
Transmembranei115 – 135HelicalSequence analysisAdd BLAST21
Transmembranei136 – 156HelicalSequence analysisAdd BLAST21
Transmembranei207 – 227HelicalSequence analysisAdd BLAST21
Transmembranei235 – 255HelicalSequence analysisAdd BLAST21
Transmembranei266 – 286HelicalSequence analysisAdd BLAST21
Transmembranei307 – 327HelicalSequence analysisAdd BLAST21
Transmembranei340 – 360HelicalSequence analysisAdd BLAST21
Transmembranei388 – 408HelicalSequence analysisAdd BLAST21
Transmembranei425 – 445HelicalSequence analysisAdd BLAST21
Transmembranei451 – 471HelicalSequence analysisAdd BLAST21
Transmembranei493 – 513HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • basolateral plasma membrane Source: UniProtKB
  • cell junction Source: UniProtKB-KW
  • endosome membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial membrane Source: UniProtKB
  • plasma membrane Source: UniProtKB
  • sperm principal piece Source: UniProtKB
  • synaptic vesicle membrane Source: UniProtKB

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasmic vesicle, Endosome, Membrane, Mitochondrion, Synapse

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. However males show reduced fertility caused by diminished sperm motility (PubMed:27010853). Deficient mice display a pathological glucose tolerance with impaired insulin secretion but normal peripheral insulin sensitivity (PubMed:23720317). Mutant have normal bone density and their bones are characterized by normal structural parameters (PubMed:20441802, PubMed:22985540).4 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003312711 – 547Sodium/hydrogen exchanger 9B2Add BLAST547

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei49PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5BKR2.
PeptideAtlasiQ5BKR2.
PRIDEiQ5BKR2.

PTM databases

PhosphoSitePlusiQ5BKR2.

Expressioni

Tissue specificityi

Widely expressed (PubMed:18000046, PubMed:18508966, PubMed:17988971). However expression seems to be restricted to specific cell types within individual organs, e.g. osteoclasts in the bone, distal tubules of the kidney or beta-cells of Langerhans islets (PubMed:18508966, PubMed:17988971, PubMed:17698421, PubMed:23720317). In sperm specifically present in the principal piece of sperm tail (at protein level) (PubMed:27010853).6 Publications

Inductioni

Not detectable during the early stages of osteoclast differentiation induced by TNFSF11/RANKL (PubMed:17698421, PubMed:17988971). Up-regulated during the later stages of osteoclast differentiation (PubMed:20441802, PubMed:18508966, PubMed:17698421, PubMed:17988971). Up-regulated in macrophages and blood mononuclear cells treated with TNFSF11/RANKL.4 Publications

Gene expression databases

BgeeiENSMUSG00000037994.
ExpressionAtlasiQ5BKR2. baseline and differential.
GenevisibleiQ5BKR2. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000060640.

Structurei

3D structure databases

ProteinModelPortaliQ5BKR2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3826. Eukaryota.
COG0025. LUCA.
GeneTreeiENSGT00390000013285.
HOGENOMiHOG000060325.
HOVERGENiHBG108184.
InParanoidiQ5BKR2.
OMAiKKVGRPA.
OrthoDBiEOG091G06PH.
TreeFamiTF319087.

Family and domain databases

InterProiView protein in InterPro
IPR006153. Cation/H_exchanger.
IPR030187. NHA2.
PANTHERiPTHR31102:SF9. PTHR31102:SF9. 1 hit.
PfamiView protein in Pfam
PF00999. Na_H_Exchanger. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5BKR2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDEDKTAEC QHSKPPTGIT HEAPPHHELQ EERVMSLRGT DRSEPTEGSN
60 70 80 90 100
LLTSGEKKPQ DSPTEPNGLQ SLRRFLACPP RGCLARVITN GTMVVLLWAM
110 120 130 140 150
VWSVTGPECL PGGNLFGIII LFYCSITGGK LFGLIKFPTL PPLPPLLGML
160 170 180 190 200
LAGFLLRNIP VINDSVRIQH KWSSSLRSIA LSVILVRAGL GLDSKALRKL
210 220 230 240 250
KGVCVRLAMG PCIVEACASA ILSHFLMGLP WQWGFILGFV VGAVSPAVVV
260 270 280 290 300
PSMLLLQEGG YGVGKGIPTL LMAAGSFDDI LAITGFNTCL GVAFSTGSTV
310 320 330 340 350
FNIFRGILEV VIGVAAGSFL GFFIQYFPSR DQDNLVWKRA FLVLGFAVLA
360 370 380 390 400
VFSSVYFSFP GSGGLCTLVM AFLAGMRWTD KKSEVEKVIA VTWDVFQPLL
410 420 430 440 450
FGLIGAEVSI VSLRAETVGL CVATLSIAVL IRILTTFLMV CFAGFNIKEK
460 470 480 490 500
IFISFAWLPK ATVQAAIGSV ALDTARSHGE KQLEDYGMDV LTVAFLAILI
510 520 530 540
TAPIGSLLIG LLGPRVLQKS EHRTEEEVQG ETSAHIQRKP EDSITEA
Length:547
Mass (Da):58,934
Last modified:July 27, 2011 - v2
Checksum:iB1F2A99BBF70BE96
GO
Isoform 2 (identifier: Q5BKR2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-252: Missing.

Note: No experimental confirmation available.
Show »
Length:295
Mass (Da):31,746
Checksum:iE028B9650DEE92B1
GO
Isoform 3 (identifier: Q5BKR2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-438: Missing.

Note: No experimental confirmation available.
Show »
Length:109
Mass (Da):11,866
Checksum:iB9CA134FB6C748D5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30Q → R in AAH90977 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0331541 – 438Missing in isoform 3. 1 PublicationAdd BLAST438
Alternative sequenceiVSP_0331551 – 252Missing in isoform 2. 1 PublicationAdd BLAST252

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029417 mRNA. Translation: BAC26441.1.
AK039655 mRNA. Translation: BAC30408.1.
AC104874 Genomic DNA. No translation available.
BC090977 mRNA. Translation: AAH90977.1.
CCDSiCCDS17854.1. [Q5BKR2-1]
RefSeqiNP_849208.4. NM_178877.6. [Q5BKR2-1]
XP_006502461.1. XM_006502398.3. [Q5BKR2-1]
XP_006502462.1. XM_006502399.3. [Q5BKR2-1]
UniGeneiMm.441764.

Genome annotation databases

EnsembliENSMUST00000051849; ENSMUSP00000060640; ENSMUSG00000037994. [Q5BKR2-1]
GeneIDi97086.
KEGGimmu:97086.
UCSCiuc008rld.2. mouse. [Q5BKR2-1]
uc008rle.2. mouse. [Q5BKR2-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK029417 mRNA. Translation: BAC26441.1.
AK039655 mRNA. Translation: BAC30408.1.
AC104874 Genomic DNA. No translation available.
BC090977 mRNA. Translation: AAH90977.1.
CCDSiCCDS17854.1. [Q5BKR2-1]
RefSeqiNP_849208.4. NM_178877.6. [Q5BKR2-1]
XP_006502461.1. XM_006502398.3. [Q5BKR2-1]
XP_006502462.1. XM_006502399.3. [Q5BKR2-1]
UniGeneiMm.441764.

3D structure databases

ProteinModelPortaliQ5BKR2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000060640.

PTM databases

PhosphoSitePlusiQ5BKR2.

Proteomic databases

PaxDbiQ5BKR2.
PeptideAtlasiQ5BKR2.
PRIDEiQ5BKR2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051849; ENSMUSP00000060640; ENSMUSG00000037994. [Q5BKR2-1]
GeneIDi97086.
KEGGimmu:97086.
UCSCiuc008rld.2. mouse. [Q5BKR2-1]
uc008rle.2. mouse. [Q5BKR2-3]

Organism-specific databases

CTDi133308.
MGIiMGI:2140077. Slc9b2.

Phylogenomic databases

eggNOGiKOG3826. Eukaryota.
COG0025. LUCA.
GeneTreeiENSGT00390000013285.
HOGENOMiHOG000060325.
HOVERGENiHBG108184.
InParanoidiQ5BKR2.
OMAiKKVGRPA.
OrthoDBiEOG091G06PH.
TreeFamiTF319087.

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Miscellaneous databases

PROiPR:Q5BKR2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037994.
ExpressionAtlasiQ5BKR2. baseline and differential.
GenevisibleiQ5BKR2. MM.

Family and domain databases

InterProiView protein in InterPro
IPR006153. Cation/H_exchanger.
IPR030187. NHA2.
PANTHERiPTHR31102:SF9. PTHR31102:SF9. 1 hit.
PfamiView protein in Pfam
PF00999. Na_H_Exchanger. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSL9B2_MOUSE
AccessioniPrimary (citable) accession number: Q5BKR2
Secondary accession number(s): Q8CA47, Q8CDX4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 27, 2011
Last modified: June 7, 2017
This is version 88 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.