Reviewed, UniProtKB/Swiss-Prot Q5BK81 (PTGR2_RAT)

Names and origin

Protein names

Recommended name:
    Prostaglandin reductase 2
      Short name=PRG-2
    EC=1.3.1.48
Alternative name(s):
    15-oxoprostaglandin 13-reductase
    Zinc-binding alcohol dehydrogenase domain-containing protein 1

Gene names

Name:	Ptgr2
Synonyms:	Zadh1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation By similarity. UniProtKB Q8VDQ1
Catalytic activity	11-alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ = (5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H. UniProtKB Q8VDQ1
Cofactor	NADPH By similarity. UniProtKB Q8VDQ1
Subunit structure	Monomer By similarity. UniProtKB Q8VDQ1
Subcellular location	Cytoplasm By similarity. UniProtKB Q29073
Sequence similarities	Belongs to the NADP-dependent oxidoreductase L4BD family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	15-oxoprostaglandin 13-oxidase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5BK81-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Gene prediction based on similarity to mouse ortholog.

Isoform 2 Ref.2 (identifier: Q5BK81-2)

The sequence of this isoform differs from the canonical sequence as follows:
     244-313: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Prostaglandin reductase 2		PRO_0000343830
Regions
	Nucleotide binding	162 – 168	7	NADP Potential
	Nucleotide binding	188 – 208	21	NADP Potential
Natural variations
	Alternative sequence	244 – 313	70	Missing in isoform 2. Ref.2		VSP_052852

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 22, 2008. Version 2. Checksum: F94578C4E45B47C6	FASTA	351	38,136
	10 20 30 40 50 60 MIIQRVVLDS RPGKNGNPVA ENFRVEEVSL PDTINEGQVR VRTLYLSVDP YMRCKMNEET 70 80 90 100 110 120 GADYLAPWQL AQVADGGGLG VIEESKHQKL AKGDFVTSFY WPWQTKAILD GNGLEKVDPQ 130 140 150 160 170 180 LVDGHLSYFL GAIGMPGLTS LIGVQEKGHV SAGSNQTMVV SGAAGACGSL AGQIGHLLGC 190 200 210 220 230 240 SRVVGICGTH EKCLFLTSEL GFDAAVNYKT GNVAEQLREA CPDGVDVYFD NVGGDISNAV 250 260 270 280 290 300 ISQMNQNSHI ILCGQISQYN KDVPYPPPLP PAVEAIQKER NITRERFMVL NYKDRFEPGI 310 320 330 340 350 LQLSQWFKEG KLKIKETVAN GLENMGVAFQ SMMTGGNIGK QIVRISEDSS P
	Isoform 2. Checksum: F780BA7811AAAE7E	FASTA	281	29,897
	10 20 30 40 50 60 MIIQRVVLDS RPGKNGNPVA ENFRVEEVSL PDTINEGQVR VRTLYLSVDP YMRCKMNEET 70 80 90 100 110 120 GADYLAPWQL AQVADGGGLG VIEESKHQKL AKGDFVTSFY WPWQTKAILD GNGLEKVDPQ 130 140 150 160 170 180 LVDGHLSYFL GAIGMPGLTS LIGVQEKGHV SAGSNQTMVV SGAAGACGSL AGQIGHLLGC 190 200 210 220 230 240 SRVVGICGTH EKCLFLTSEL GFDAAVNYKT GNVAEQLREA CPDGVDVYFD NVGGDISNAV 250 260 270 280 ISQIKETVAN GLENMGVAFQ SMMTGGNIGK QIVRISEDSS P

References

[1]

"Genome sequence of the Brown Norway rat yields insights into mammalian evolution." Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M. , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S. Nature 428:493-521(2004) [PubMed: 15057822] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.

[2]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: Brown Norway. Tissue: Heart.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AABR03048578 Genomic DNA. No translation available. AABR03050041 Genomic DNA. No translation available. BC091173 mRNA. Translation: AAH91173.1.
IPI	IPI00365859. IPI00900498.
RefSeq	NP_001015009.1.
UniGene	Rn.203285
3D structure databases
SMR	Q5BK81. Positions 1-246.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000058095; ENSRNOP00000054903; ENSRNOG00000038166; Rattus norvegicus. [Genome view]
GeneID	299194.
KEGG	rno:299194.
UCSC	NM_001015009. rat.
Organism-specific databases
CTD	299194.
RGD	131051. Zadh1.
Phylogenomic databases
HOVERGEN	Q5BK81.
OMA	GLENMGV.
Gene expression databases
ArrayExpress	Q5BK81.
Genevestigator	Q5BK81.
Family and domain databases
InterPro	IPR013149. ADH_Zn-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00107. ADH_zinc_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	644957.

Entry information

Entry name

PTGR2_RAT

Accession

Primary (citable) accession number: Q5BK81

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	July 22, 2008
Last modified:	November 3, 2009
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)