Q5BK63 (NDUA9_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial

Alternative name(s):
Complex I-39kD
Short name=CI-39kD
NADH-ubiquinone oxidoreductase 39 kDa subunit
Sperm flagella protein 3

Gene names

Name:

Ndufa9

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Cofactor	Binds 1 FAD per subunit.
Subunit structure	Complex I is composed of 45 different subunits. This a component of the hydrophobic protein fraction By similarity. Interacts with BLOC1S1 By similarity. Interacts with SLC2A4. Ref.3
Subcellular location	Mitochondrion matrix.
Tissue specificity	Expressed by the principal cells of the epididymis. Detected in flagella of epididymal sperm (at protein level). Ref.4
Post-translational modification	Acetylated on lysine residues. BLOC1S1 is required for acetylation By similarity.
Sequence similarities	Belongs to the complex I NDUFA9 subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD Flavoprotein
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: Compara mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 35	35	Mitochondrion By similarity
	Chain	36 – 377	342	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial		PRO_0000308374
Amino acid modifications
	Modified residue	370	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5BK63 [UniParc]. Last modified January 11, 2011. Version 2. Checksum: 578C531D5223CD5C	FASTA	377	42,559
	10 20 30 40 50 60 MAAAVRFQVV RALPMSRPAI SAAATSVFCS SSHRQLHHAV IPHGKGGRSS VSGVVATVFG 70 80 90 100 110 120 ATGFLGRYVV NHLGRMGSQV IIPYRCDIYD TMHLRLMGDL GQLIFLEWDA RDKDSIRKAV 130 140 150 160 170 180 QHSNVVINLI GREWETRNFD FEDVFVNIPR AIAQASKEAG VERFIHVSHL NASMKSSAKS 190 200 210 220 230 240 LRSKAVGEKE VRTVFPDAII IRPSDMFGRE DRFLNHFANY RWFLAVPLVS LGFKTVKQPV 250 260 270 280 290 300 YVADVSKGIA NATKNPDAIG KTFAFTGPNR YLLFHLVKYI FGMTHRTFIP YPLPRFVYSW 310 320 330 340 350 360 IGRLFGLSPF EPWTTKDKVE RIHISDVMAT DLPGLEDLGV QPTPLELKSI EVLRRHRTYR 370 WLSSEIEETK PAKTVNY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver and Pituitary anterior lobe.
[3]	"Insulin-dependent interactions of proteins with GLUT4 revealed through stable isotope labeling by amino acids in cell culture (SILAC)." Foster L.J., Rudich A., Talior I., Patel N., Huang X., Furtado L.M., Bilan P.J., Mann M., Klip A. J. Proteome Res. 5:64-75(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SLC2A4.
[4]	"Identification of novel immunodominant epididymal sperm proteins using combinatorial approach." Khan S.A., Suryawanshi A.R., Ranpura S.A., Jadhav S.V., Khole V.V. Reproduction 138:81-93(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY. Strain: Holtzman. Tissue: Epididymis and Sperm.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH473964 Genomic DNA. Translation: EDM01826.1. BC091192 mRNA. Translation: AAH91192.1. BC168777 mRNA. Translation: AAI68777.1.
IPI	IPI00358441.
RefSeq	NP_001094222.1. NM_001100752.1.
UniGene	Rn.107963.
3D structure databases
HSSP	HSSP built from PDB template 2A35 based on UniProtKB Q9HX10.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5BK63. 1 interaction.
STRING	10116.ENSRNOP00000034135.
Proteomic databases
PaxDb	Q5BK63.
PRIDE	Q5BK63.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000037176; ENSRNOP00000034135; ENSRNOG00000026930.
GeneID	362440.
KEGG	rno:362440.
UCSC	RGD:1307307. rat.
Organism-specific databases
CTD	4704.
RGD	1307307. Ndufa9.
Phylogenomic databases
eggNOG	COG0702.
GeneTree	ENSGT00390000006865.
HOGENOM	HOG000168008.
HOVERGEN	HBG006546.
InParanoid	Q5BK63.
KO	K03953.
OMA	YIYAVAH.
OrthoDB	EOG4RJG1Z.
Gene expression databases
Genevestigator	Q5BK63.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR016040. NAD(P)-bd_dom. IPR008030. NmrA. [Graphical view]
Pfam	PF05368. NmrA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	679945.

Entry information

Entry name

NDUA9_RAT

Accession

Primary (citable) accession number: Q5BK63
Secondary accession number(s): B5DER7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	January 11, 2011
Last modified:	April 3, 2013
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program