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Protein

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial

Gene

Ndufa9

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Cofactori

FADCuratedNote: Binds 1 FAD per subunit.Curated

GO - Biological processi

  1. oxidation-reduction process Source: UniProtKB-KW
  2. response to glucose Source: RGD
Complete GO annotation...

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

ReactomeiREACT_262146. Respiratory electron transport.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial
Alternative name(s):
Complex I-39kD
Short name:
CI-39kD
NADH-ubiquinone oxidoreductase 39 kDa subunit
Sperm flagella protein 3
Gene namesi
Name:Ndufa9Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi1307307. Ndufa9.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
  2. mitochondrial respiratory chain complex I Source: Ensembl
  3. mitochondrion Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3535MitochondrionBy similarityAdd
BLAST
Chaini36 – 377342NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrialPRO_0000308374Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei175 – 1751N6-succinyllysineBy similarity
Modified residuei189 – 1891N6-acetyllysineBy similarity
Modified residuei370 – 3701N6-acetyllysineBy similarity

Post-translational modificationi

Acetylated on lysine residues. BLOC1S1 is required for acetylation (By similarity).By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ5BK63.
PRIDEiQ5BK63.

Expressioni

Tissue specificityi

Expressed by the principal cells of the epididymis. Detected in flagella of epididymal sperm (at protein level).1 Publication

Gene expression databases

GenevestigatoriQ5BK63.

Interactioni

Subunit structurei

Complex I is composed of 45 different subunits. This a component of the hydrophobic protein fraction (By similarity). Interacts with BLOC1S1 (By similarity). Interacts with SLC2A4.By similarity1 Publication

Protein-protein interaction databases

IntActiQ5BK63. 1 interaction.
STRINGi10116.ENSRNOP00000034135.

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I NDUFA9 subunit family.Sequence Analysis

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0702.
GeneTreeiENSGT00390000006865.
HOGENOMiHOG000168008.
HOVERGENiHBG006546.
InParanoidiQ5BK63.
KOiK03953.
OMAiGREWETK.
OrthoDBiEOG7TF795.
PhylomeDBiQ5BK63.
TreeFamiTF105961.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR008030. NmrA-like.
[Graphical view]
PfamiPF05368. NmrA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5BK63-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAAVRFQVV RALPMSRPAI SAAATSVFCS SSHRQLHHAV IPHGKGGRSS
60 70 80 90 100
VSGVVATVFG ATGFLGRYVV NHLGRMGSQV IIPYRCDIYD TMHLRLMGDL
110 120 130 140 150
GQLIFLEWDA RDKDSIRKAV QHSNVVINLI GREWETRNFD FEDVFVNIPR
160 170 180 190 200
AIAQASKEAG VERFIHVSHL NASMKSSAKS LRSKAVGEKE VRTVFPDAII
210 220 230 240 250
IRPSDMFGRE DRFLNHFANY RWFLAVPLVS LGFKTVKQPV YVADVSKGIA
260 270 280 290 300
NATKNPDAIG KTFAFTGPNR YLLFHLVKYI FGMTHRTFIP YPLPRFVYSW
310 320 330 340 350
IGRLFGLSPF EPWTTKDKVE RIHISDVMAT DLPGLEDLGV QPTPLELKSI
360 370
EVLRRHRTYR WLSSEIEETK PAKTVNY
Length:377
Mass (Da):42,559
Last modified:January 11, 2011 - v2
Checksum:i578C531D5223CD5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473964 Genomic DNA. Translation: EDM01826.1.
BC091192 mRNA. Translation: AAH91192.1.
BC168777 mRNA. Translation: AAI68777.1.
RefSeqiNP_001094222.1. NM_001100752.1.
UniGeneiRn.107963.

Genome annotation databases

EnsembliENSRNOT00000037176; ENSRNOP00000034135; ENSRNOG00000026930.
GeneIDi362440.
KEGGirno:362440.
UCSCiRGD:1307307. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473964 Genomic DNA. Translation: EDM01826.1.
BC091192 mRNA. Translation: AAH91192.1.
BC168777 mRNA. Translation: AAI68777.1.
RefSeqiNP_001094222.1. NM_001100752.1.
UniGeneiRn.107963.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ5BK63. 1 interaction.
STRINGi10116.ENSRNOP00000034135.

Proteomic databases

PaxDbiQ5BK63.
PRIDEiQ5BK63.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000037176; ENSRNOP00000034135; ENSRNOG00000026930.
GeneIDi362440.
KEGGirno:362440.
UCSCiRGD:1307307. rat.

Organism-specific databases

CTDi4704.
RGDi1307307. Ndufa9.

Phylogenomic databases

eggNOGiCOG0702.
GeneTreeiENSGT00390000006865.
HOGENOMiHOG000168008.
HOVERGENiHBG006546.
InParanoidiQ5BK63.
KOiK03953.
OMAiGREWETK.
OrthoDBiEOG7TF795.
PhylomeDBiQ5BK63.
TreeFamiTF105961.

Enzyme and pathway databases

ReactomeiREACT_262146. Respiratory electron transport.

Miscellaneous databases

NextBioi679945.
PROiQ5BK63.

Gene expression databases

GenevestigatoriQ5BK63.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR008030. NmrA-like.
[Graphical view]
PfamiPF05368. NmrA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: LiverImported and Pituitary anterior lobe.
  3. "Insulin-dependent interactions of proteins with GLUT4 revealed through stable isotope labeling by amino acids in cell culture (SILAC)."
    Foster L.J., Rudich A., Talior I., Patel N., Huang X., Furtado L.M., Bilan P.J., Mann M., Klip A.
    J. Proteome Res. 5:64-75(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SLC2A4.
  4. "Identification of novel immunodominant epididymal sperm proteins using combinatorial approach."
    Khan S.A., Suryawanshi A.R., Ranpura S.A., Jadhav S.V., Khole V.V.
    Reproduction 138:81-93(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, TISSUE SPECIFICITY.
    Strain: Holtzman.
    Tissue: Epididymis and Sperm.

Entry informationi

Entry nameiNDUA9_RAT
AccessioniPrimary (citable) accession number: Q5BK63
Secondary accession number(s): B5DER7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: January 11, 2011
Last modified: January 7, 2015
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.