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Protein

Josephin-1

Gene

Josd1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei36NucleophilePROSITE-ProRule annotation1
Active sitei139Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC86.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Josephin-1 (EC:3.4.19.12)
Alternative name(s):
Josephin domain-containing protein 1
Gene namesi
Name:Josd1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1305388. Josd1.

Subcellular locationi

  • Cell membrane By similarity
  • Cytoplasm By similarity

  • Note: Ubiquitination increases localization the plasma membrane. In the cytosol, the unubiquitinated form may be associated with the cytoskeleton via ACTB-binding.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000538421 – 202Josephin-1Add BLAST202

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphoserineBy similarity1

Post-translational modificationi

Monoubiquitinated. Ubiquitination activates deubiquitination activity in vitro.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5BJY4.

PTM databases

iPTMnetiQ5BJY4.
PhosphoSitePlusiQ5BJY4.

Expressioni

Gene expression databases

BgeeiENSRNOG00000014440.
GenevisibleiQ5BJY4. RN.

Interactioni

Subunit structurei

Interacts with beta-actin/ACTB.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019612.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 202JosephinPROSITE-ProRule annotationAdd BLAST180

Sequence similaritiesi

Contains 1 Josephin domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2934. Eukaryota.
ENOG4111I4J. LUCA.
GeneTreeiENSGT00390000009228.
HOGENOMiHOG000005731.
HOVERGENiHBG039523.
InParanoidiQ5BJY4.
KOiK15235.
OMAiREVGGTY.
OrthoDBiEOG091G0NAU.
PhylomeDBiQ5BJY4.
TreeFamiTF313660.

Family and domain databases

InterProiIPR006155. Josephin.
[Graphical view]
PfamiPF02099. Josephin. 1 hit.
[Graphical view]
SMARTiSM01246. Josephin. 1 hit.
[Graphical view]
PROSITEiPS50957. JOSEPHIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5BJY4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCMPWKGDK AKSESLELPQ AAPPQIYHEK QRRELCALHA LNNVFQDSNA
60 70 80 90 100
FTRETLQEIF QRLSPNTVVT PHKKSMLGNG NYDVNVIMAA LQTKGYEAVW
110 120 130 140 150
WDKRRDVGVI ALANVMGFIM NLPSSLCWGP LKLPLKRQHW ICVREVGGAY
160 170 180 190 200
YNLDSKLKMP EWIGGESELR KFLKYHLRGK NCELLLVVPE EVEAHQSWRA

DV
Length:202
Mass (Da):23,178
Last modified:April 12, 2005 - v1
Checksum:iA4762D1C78E56623
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC091280 mRNA. Translation: AAH91280.1.
RefSeqiNP_001020180.1. NM_001025009.1.
UniGeneiRn.2495.

Genome annotation databases

EnsembliENSRNOT00000019612; ENSRNOP00000019612; ENSRNOG00000014440.
GeneIDi315134.
KEGGirno:315134.
UCSCiRGD:1305388. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC091280 mRNA. Translation: AAH91280.1.
RefSeqiNP_001020180.1. NM_001025009.1.
UniGeneiRn.2495.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019612.

Protein family/group databases

MEROPSiC86.004.

PTM databases

iPTMnetiQ5BJY4.
PhosphoSitePlusiQ5BJY4.

Proteomic databases

PaxDbiQ5BJY4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000019612; ENSRNOP00000019612; ENSRNOG00000014440.
GeneIDi315134.
KEGGirno:315134.
UCSCiRGD:1305388. rat.

Organism-specific databases

CTDi9929.
RGDi1305388. Josd1.

Phylogenomic databases

eggNOGiKOG2934. Eukaryota.
ENOG4111I4J. LUCA.
GeneTreeiENSGT00390000009228.
HOGENOMiHOG000005731.
HOVERGENiHBG039523.
InParanoidiQ5BJY4.
KOiK15235.
OMAiREVGGTY.
OrthoDBiEOG091G0NAU.
PhylomeDBiQ5BJY4.
TreeFamiTF313660.

Miscellaneous databases

PROiQ5BJY4.

Gene expression databases

BgeeiENSRNOG00000014440.
GenevisibleiQ5BJY4. RN.

Family and domain databases

InterProiIPR006155. Josephin.
[Graphical view]
PfamiPF02099. Josephin. 1 hit.
[Graphical view]
SMARTiSM01246. Josephin. 1 hit.
[Graphical view]
PROSITEiPS50957. JOSEPHIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiJOS1_RAT
AccessioniPrimary (citable) accession number: Q5BJY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: April 12, 2005
Last modified: November 30, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.