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Protein

Josephin-1

Gene

Josd1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei36NucleophilePROSITE-ProRule annotation1
Active sitei139Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-RNO-5689877 Josephin domain DUBs

Protein family/group databases

MEROPSiC86.004

Names & Taxonomyi

Protein namesi
Recommended name:
Josephin-1 (EC:3.4.19.12)
Alternative name(s):
Josephin domain-containing protein 1
Gene namesi
Name:Josd1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1305388 Josd1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000538421 – 202Josephin-1Add BLAST202

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphoserineBy similarity1

Post-translational modificationi

Monoubiquitinated. Ubiquitination activates deubiquitination activity in vitro.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5BJY4

PTM databases

iPTMnetiQ5BJY4
PhosphoSitePlusiQ5BJY4

Expressioni

Gene expression databases

BgeeiENSRNOG00000014440
GenevisibleiQ5BJY4 RN

Interactioni

Subunit structurei

Interacts with beta-actin/ACTB.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019612

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 202JosephinPROSITE-ProRule annotationAdd BLAST180

Phylogenomic databases

eggNOGiKOG2934 Eukaryota
ENOG4111I4J LUCA
GeneTreeiENSGT00390000009228
HOGENOMiHOG000005731
HOVERGENiHBG039523
InParanoidiQ5BJY4
KOiK15235
OMAiREVGGTY
OrthoDBiEOG091G0NAU
PhylomeDBiQ5BJY4
TreeFamiTF313660

Family and domain databases

InterProiView protein in InterPro
IPR006155 Josephin
PfamiView protein in Pfam
PF02099 Josephin, 1 hit
SMARTiView protein in SMART
SM01246 Josephin, 1 hit
PROSITEiView protein in PROSITE
PS50957 JOSEPHIN, 1 hit

Sequencei

Sequence statusi: Complete.

Q5BJY4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCMPWKGDK AKSESLELPQ AAPPQIYHEK QRRELCALHA LNNVFQDSNA
60 70 80 90 100
FTRETLQEIF QRLSPNTVVT PHKKSMLGNG NYDVNVIMAA LQTKGYEAVW
110 120 130 140 150
WDKRRDVGVI ALANVMGFIM NLPSSLCWGP LKLPLKRQHW ICVREVGGAY
160 170 180 190 200
YNLDSKLKMP EWIGGESELR KFLKYHLRGK NCELLLVVPE EVEAHQSWRA

DV
Length:202
Mass (Da):23,178
Last modified:April 12, 2005 - v1
Checksum:iA4762D1C78E56623
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC091280 mRNA Translation: AAH91280.1
RefSeqiNP_001020180.1, NM_001025009.1
UniGeneiRn.2495

Genome annotation databases

EnsembliENSRNOT00000019612; ENSRNOP00000019612; ENSRNOG00000014440
GeneIDi315134
KEGGirno:315134
UCSCiRGD:1305388 rat

Similar proteinsi

Entry informationi

Entry nameiJOS1_RAT
AccessioniPrimary (citable) accession number: Q5BJY4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: April 12, 2005
Last modified: May 23, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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