Q5BJY4 (JOS1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Josephin-1
EC=3.4.19.12

Alternative name(s):
Josephin domain-containing protein 1

Gene names

Name:

Josd1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Has low protease activity towards poly-ubiquitin chains (in vitro). May act as a deubiquitinating enzyme By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Contains 1 Josephin domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	omega peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	Josephin-1		PRO_0000053842
Regions
	Domain	23 – 202	180	Josephin
Sites
	Active site	36	1	Nucleophile By similarity
	Active site	139	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5BJY4 [UniParc]. Last modified April 12, 2005. Version 1. Checksum: A4762D1C78E56623	FASTA	202	23,178
	10 20 30 40 50 60 MSCMPWKGDK AKSESLELPQ AAPPQIYHEK QRRELCALHA LNNVFQDSNA FTRETLQEIF 70 80 90 100 110 120 QRLSPNTVVT PHKKSMLGNG NYDVNVIMAA LQTKGYEAVW WDKRRDVGVI ALANVMGFIM 130 140 150 160 170 180 NLPSSLCWGP LKLPLKRQHW ICVREVGGAY YNLDSKLKMP EWIGGESELR KFLKYHLRGK 190 200 NCELLLVVPE EVEAHQSWRA DV

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thymus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC091280 mRNA. Translation: AAH91280.1.
IPI	IPI00202188.
RefSeq	NP_001020180.1. NM_001025009.1.
UniGene	Rn.2495.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000019612.
Protein family/group databases
MEROPS	C86.004.
PTM databases
PhosphoSite	Q5BJY4.
Proteomic databases
PaxDb	Q5BJY4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000019612; ENSRNOP00000019612; ENSRNOG00000014440.
GeneID	315134.
KEGG	rno:315134.
UCSC	RGD:1305388. rat.
Organism-specific databases
CTD	9929.
RGD	1305388. Josd1.
Phylogenomic databases
eggNOG	NOG321724.
GeneTree	ENSGT00390000009228.
HOGENOM	HOG000005731.
HOVERGEN	HBG039523.
InParanoid	Q5BJY4.
KO	K15235.
OMA	IWFDKRK.
OrthoDB	EOG40P47Z.
Gene expression databases
Genevestigator	Q5BJY4.
GermOnline	ENSRNOG00000014440. Rattus norvegicus.
Family and domain databases
InterPro	IPR006155. Josephin. [Graphical view]
Pfam	PF02099. Josephin. 1 hit. [Graphical view]
PROSITE	PS50957. JOSEPHIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	668731.

Entry information

Entry name

JOS1_RAT

Accession

Primary (citable) accession number: Q5BJY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	April 12, 2005
Last modified:	April 3, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program