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Q5BJY4

- JOS1_RAT

UniProt

Q5BJY4 - JOS1_RAT

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Protein
Josephin-1
Gene
Josd1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei36 – 361Nucleophile By similarity
Active sitei139 – 1391Proton acceptor By similarity

GO - Molecular functioni

  1. omega peptidase activity Source: InterPro
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase, Protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC86.004.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Josephin-1 (EC:3.4.19.12)
    Alternative name(s):
    Josephin domain-containing protein 1
    Gene namesi
    Name:Josd1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 7

    Organism-specific databases

    RGDi1305388. Josd1.

    Subcellular locationi

    Cell membrane By similarity. Cytoplasm By similarity
    Note: Ubiquitination increases localization the plasma membrane By similarity. In the cytosol, the unubiquitinated form may be associated with the cytoskeleton via ACTB-binding By similarity.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. plasma membrane Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 202202Josephin-1
    PRO_0000053842Add
    BLAST

    Post-translational modificationi

    Monoubiquitinated By similarity. Ubiquitination activates deubiquitination activity in vitro By similarity.

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PaxDbiQ5BJY4.

    PTM databases

    PhosphoSiteiQ5BJY4.

    Expressioni

    Gene expression databases

    GenevestigatoriQ5BJY4.

    Interactioni

    Subunit structurei

    Interacts with beta-actin/ACTB By similarity.

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000019612.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini23 – 202180Josephin
    Add
    BLAST

    Sequence similaritiesi

    Contains 1 Josephin domain.

    Phylogenomic databases

    eggNOGiNOG321724.
    GeneTreeiENSGT00390000009228.
    HOGENOMiHOG000005731.
    HOVERGENiHBG039523.
    InParanoidiQ5BJY4.
    KOiK15235.
    OMAiREVGGTY.
    OrthoDBiEOG75B871.
    PhylomeDBiQ5BJY4.
    TreeFamiTF313660.

    Family and domain databases

    InterProiIPR006155. Josephin.
    [Graphical view]
    PfamiPF02099. Josephin. 1 hit.
    [Graphical view]
    PROSITEiPS50957. JOSEPHIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5BJY4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSCMPWKGDK AKSESLELPQ AAPPQIYHEK QRRELCALHA LNNVFQDSNA    50
    FTRETLQEIF QRLSPNTVVT PHKKSMLGNG NYDVNVIMAA LQTKGYEAVW 100
    WDKRRDVGVI ALANVMGFIM NLPSSLCWGP LKLPLKRQHW ICVREVGGAY 150
    YNLDSKLKMP EWIGGESELR KFLKYHLRGK NCELLLVVPE EVEAHQSWRA 200
    DV 202
    Length:202
    Mass (Da):23,178
    Last modified:April 12, 2005 - v1
    Checksum:iA4762D1C78E56623
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC091280 mRNA. Translation: AAH91280.1.
    RefSeqiNP_001020180.1. NM_001025009.1.
    UniGeneiRn.2495.

    Genome annotation databases

    EnsembliENSRNOT00000019612; ENSRNOP00000019612; ENSRNOG00000014440.
    GeneIDi315134.
    KEGGirno:315134.
    UCSCiRGD:1305388. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC091280 mRNA. Translation: AAH91280.1 .
    RefSeqi NP_001020180.1. NM_001025009.1.
    UniGenei Rn.2495.

    3D structure databases

    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000019612.

    Protein family/group databases

    MEROPSi C86.004.

    PTM databases

    PhosphoSitei Q5BJY4.

    Proteomic databases

    PaxDbi Q5BJY4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000019612 ; ENSRNOP00000019612 ; ENSRNOG00000014440 .
    GeneIDi 315134.
    KEGGi rno:315134.
    UCSCi RGD:1305388. rat.

    Organism-specific databases

    CTDi 9929.
    RGDi 1305388. Josd1.

    Phylogenomic databases

    eggNOGi NOG321724.
    GeneTreei ENSGT00390000009228.
    HOGENOMi HOG000005731.
    HOVERGENi HBG039523.
    InParanoidi Q5BJY4.
    KOi K15235.
    OMAi REVGGTY.
    OrthoDBi EOG75B871.
    PhylomeDBi Q5BJY4.
    TreeFami TF313660.

    Miscellaneous databases

    NextBioi 668731.
    PROi Q5BJY4.

    Gene expression databases

    Genevestigatori Q5BJY4.

    Family and domain databases

    InterProi IPR006155. Josephin.
    [Graphical view ]
    Pfami PF02099. Josephin. 1 hit.
    [Graphical view ]
    PROSITEi PS50957. JOSEPHIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Thymus.

    Entry informationi

    Entry nameiJOS1_RAT
    AccessioniPrimary (citable) accession number: Q5BJY4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2005
    Last sequence update: April 12, 2005
    Last modified: May 14, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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