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Protein

N-terminal Xaa-Pro-Lys N-methyltransferase 1

Gene

Ntmt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by METTL11B-mediated monomethylation. Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1 (By similarity).By similarity

Catalytic activityi

3 S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein].
2 S-adenosyl-L-methionine + N-terminal-PPK-[protein] = 2 S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein].

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Binding sitei74 – 741S-adenosyl-L-methionineBy similarity
Binding sitei135 – 1351S-adenosyl-L-methionine; via carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
N-terminal Xaa-Pro-Lys N-methyltransferase 1 (EC:2.1.1.244)
Alternative name(s):
Alpha N-terminal protein methyltransferase 1A
Methyltransferase-like protein 11A
X-Pro-Lys N-terminal protein methyltransferase 1A
Short name:
NTM1A
Cleaved into the following chain:
Gene namesi
Name:Ntmt1
Synonyms:Mettl11a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1306582. Ntmt1.

Subcellular locationi

  • Nucleus By similarity

  • Note: Predominantly nuclear.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 223223N-terminal Xaa-Pro-Lys N-methyltransferase 1PRO_0000119290Add
BLAST
Initiator methionineiRemoved; alternateBy similarity
Chaini2 – 223222N-terminal Xaa-Pro-Lys N-methyltransferase 1, N-terminally processedPRO_0000423230Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei2 – 21N-acetylthreonine; in N-terminal Xaa-Pro-Lys N-methyltransferase 1, N-terminally processedBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ5BJX0.
PRIDEiQ5BJX0.

Expressioni

Gene expression databases

GenevisibleiQ5BJX0. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000035921.

Structurei

3D structure databases

ProteinModelPortaliQ5BJX0.
SMRiQ5BJX0. Positions 2-223.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni91 – 933S-adenosyl-L-methionine bindingBy similarity
Regioni119 – 1202S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3178. Eukaryota.
ENOG410XS7T. LUCA.
GeneTreeiENSGT00390000008371.
HOVERGENiHBG054992.
InParanoidiQ5BJX0.
KOiK16219.
OMAiGHLTDDH.
OrthoDBiEOG7ZPNM6.
PhylomeDBiQ5BJX0.
TreeFamiTF314174.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR008576. MeTrfase_NTM1.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR12753. PTHR12753. 1 hit.
PfamiPF05891. Methyltransf_PK. 1 hit.
[Graphical view]
PIRSFiPIRSF016958. DUF858_MeTrfase_lik. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5BJX0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSEVIEDEK QFYSKAKTYW KQIPPTVDGM LGGYGHISNI DLNSSRKFLQ
60 70 80 90 100
RFLREGPNKT GTSCALDCGA GIGRITKRLL LPLFRVVDMV DVTEDFLAKA
110 120 130 140 150
KTYLGEEGKR VRNYFCCGLQ DFSPEPSSYD VIWIQWVIGH LTDQHLAEFL
160 170 180 190 200
RRCRRGLRPN GIIVIKDNMA QEGVILDDVD SSVCRDLEVV RRIIRSAGLS
210 220
LLAEERQENL PDEIYHVYSF ALR
Length:223
Mass (Da):25,464
Last modified:January 23, 2007 - v3
Checksum:i04A1CD96034A7A51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC091294 mRNA. Translation: AAH91294.1.
RefSeqiNP_001020190.1. NM_001025019.1.
XP_008759892.1. XM_008761670.1.
UniGeneiRn.12306.

Genome annotation databases

EnsembliENSRNOT00000035805; ENSRNOP00000035921; ENSRNOG00000024809.
GeneIDi362103.
KEGGirno:362103.
UCSCiRGD:1306582. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC091294 mRNA. Translation: AAH91294.1.
RefSeqiNP_001020190.1. NM_001025019.1.
XP_008759892.1. XM_008761670.1.
UniGeneiRn.12306.

3D structure databases

ProteinModelPortaliQ5BJX0.
SMRiQ5BJX0. Positions 2-223.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000035921.

Proteomic databases

PaxDbiQ5BJX0.
PRIDEiQ5BJX0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000035805; ENSRNOP00000035921; ENSRNOG00000024809.
GeneIDi362103.
KEGGirno:362103.
UCSCiRGD:1306582. rat.

Organism-specific databases

CTDi28989.
RGDi1306582. Ntmt1.

Phylogenomic databases

eggNOGiKOG3178. Eukaryota.
ENOG410XS7T. LUCA.
GeneTreeiENSGT00390000008371.
HOVERGENiHBG054992.
InParanoidiQ5BJX0.
KOiK16219.
OMAiGHLTDDH.
OrthoDBiEOG7ZPNM6.
PhylomeDBiQ5BJX0.
TreeFamiTF314174.

Miscellaneous databases

PROiQ5BJX0.

Gene expression databases

GenevisibleiQ5BJX0. RN.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR008576. MeTrfase_NTM1.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR12753. PTHR12753. 1 hit.
PfamiPF05891. Methyltransf_PK. 1 hit.
[Graphical view]
PIRSFiPIRSF016958. DUF858_MeTrfase_lik. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiNTM1A_RAT
AccessioniPrimary (citable) accession number: Q5BJX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.