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Protein

N-terminal Xaa-Pro-Lys N-methyltransferase 1

Gene

Ntmt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Distributive alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Gly/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of the exposed alpha-amino group of the Ala, Gly or Ser residue in the [Ala/Gly/Ser]-Pro-Lys motif and mono- or di-methylation of Pro in the Pro-Pro-Lys motif. Some of the substrates may be primed by METTL11B-mediated monomethylation. Catalyzes the trimethylation of the N-terminal Gly in CENPA (after removal of Met-1). Responsible for the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation via its action on RCC1.By similarity

Catalytic activityi

3 S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein].By similarity
2 S-adenosyl-L-methionine + N-terminal-PPK-[protein] = 2 S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein].By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei74S-adenosyl-L-methionineBy similarity1
Binding sitei135S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
N-terminal Xaa-Pro-Lys N-methyltransferase 1 (EC:2.1.1.244By similarity)
Alternative name(s):
Alpha N-terminal protein methyltransferase 1A
Methyltransferase-like protein 11A
X-Pro-Lys N-terminal protein methyltransferase 1A
Short name:
NTM1A
Cleaved into the following chain:
Gene namesi
Name:Ntmt1
Synonyms:Mettl11a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi1306582 Ntmt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004232301 – 223N-terminal Xaa-Pro-Lys N-methyltransferase 1Add BLAST223
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00001192902 – 223N-terminal Xaa-Pro-Lys N-methyltransferase 1, N-terminally processedAdd BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylthreonine; in N-terminal Xaa-Pro-Lys N-methyltransferase 1, N-terminally processedBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ5BJX0
PRIDEiQ5BJX0

PTM databases

PhosphoSitePlusiQ5BJX0

Expressioni

Gene expression databases

BgeeiENSRNOG00000024809
GenevisibleiQ5BJX0 RN

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000035921

Structurei

3D structure databases

ProteinModelPortaliQ5BJX0
SMRiQ5BJX0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni91 – 93S-adenosyl-L-methionine bindingBy similarity3
Regioni119 – 120S-adenosyl-L-methionine bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3178 Eukaryota
ENOG410XS7T LUCA
GeneTreeiENSGT00390000008371
HOVERGENiHBG054992
InParanoidiQ5BJX0
KOiK16219
OMAiPVYMIAC
OrthoDBiEOG091G0NA7
PhylomeDBiQ5BJX0
TreeFamiTF314174

Family and domain databases

InterProiView protein in InterPro
IPR008576 MeTrfase_NTM1
IPR029063 SAM-dependent_MTases
PANTHERiPTHR12753 PTHR12753, 1 hit
PfamiView protein in Pfam
PF05891 Methyltransf_PK, 1 hit
PIRSFiPIRSF016958 DUF858_MeTrfase_lik, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5BJX0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSEVIEDEK QFYSKAKTYW KQIPPTVDGM LGGYGHISNI DLNSSRKFLQ
60 70 80 90 100
RFLREGPNKT GTSCALDCGA GIGRITKRLL LPLFRVVDMV DVTEDFLAKA
110 120 130 140 150
KTYLGEEGKR VRNYFCCGLQ DFSPEPSSYD VIWIQWVIGH LTDQHLAEFL
160 170 180 190 200
RRCRRGLRPN GIIVIKDNMA QEGVILDDVD SSVCRDLEVV RRIIRSAGLS
210 220
LLAEERQENL PDEIYHVYSF ALR
Length:223
Mass (Da):25,464
Last modified:January 23, 2007 - v3
Checksum:i04A1CD96034A7A51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC091294 mRNA Translation: AAH91294.1
RefSeqiNP_001020190.1, NM_001025019.1
XP_008759892.1, XM_008761670.2
UniGeneiRn.12306

Genome annotation databases

EnsembliENSRNOT00000035805; ENSRNOP00000035921; ENSRNOG00000024809
GeneIDi362103
KEGGirno:362103
UCSCiRGD:1306582 rat

Similar proteinsi

Entry informationi

Entry nameiNTM1A_RAT
AccessioniPrimary (citable) accession number: Q5BJX0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 103 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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