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Protein

Wiskott-Aldrich syndrome protein family member 1

Gene

Wasf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex (By similarity).By similarity

GO - Biological processi

  1. actin cytoskeleton organization Source: InterPro
  2. lamellipodium morphogenesis Source: Ensembl
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Wiskott-Aldrich syndrome protein family member 1
Short name:
WASP family protein member 1
Alternative name(s):
Protein WAVE-1
Gene namesi
Name:Wasf1
Synonyms:Wave1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 20

Organism-specific databases

RGDi1561954. Wasf1.

Subcellular locationi

Cell junctionsynapse 1 Publication. Cytoplasmcytoskeleton By similarity
Note: Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas (By similarity).By similarity

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. cytoskeleton Source: UniProtKB-SubCell
  3. lamellipodium Source: Ensembl
  4. mitochondrial outer membrane Source: Ensembl
  5. synapse Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 559559Wiskott-Aldrich syndrome protein family member 1PRO_0000314291Add
BLAST

Proteomic databases

PRIDEiQ5BJU7.

PTM databases

PhosphoSiteiQ5BJU7.

Expressioni

Tissue specificityi

Expressed in hippocampal neurons (at protein level).1 Publication

Gene expression databases

GenevestigatoriQ5BJU7.

Interactioni

Subunit structurei

Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterdimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1. Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Interacts with SHANK3; the interaction mediates the association of SHANK3 with the WAVE1 complex. Interacts with ABI1 (via N-terminus).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ActbP607112EBI-7269229,EBI-349272
Gnai3P087532EBI-7269229,EBI-874897
Gnb1P543112EBI-7269229,EBI-917779

Protein-protein interaction databases

BioGridi254741. 3 interactions.
IntActiQ5BJU7. 6 interactions.
MINTiMINT-1795575.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini497 – 51418WH2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi278 – 2836Poly-Pro
Compositional biasi322 – 33211Poly-ProAdd
BLAST
Compositional biasi348 – 36114Poly-ProAdd
BLAST
Compositional biasi369 – 3746Poly-Pro
Compositional biasi424 – 43512Poly-ProAdd
BLAST

Domaini

Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain.By similarity

Sequence similaritiesi

Belongs to the SCAR/WAVE family.Curated
Contains 1 WH2 domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00730000110593.
HOVERGENiHBG058482.
InParanoidiQ5BJU7.
KOiK05753.
OMAiQLFDRRT.
OrthoDBiEOG7VHSXK.
PhylomeDBiQ5BJU7.

Family and domain databases

InterProiIPR028288. SCAR/WAVE_fam.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR12902. PTHR12902. 1 hit.
PfamiPF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00246. WH2. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5BJU7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPLVKRNIDP RHLCHTALPR GIKNELECVT NISLANIIRQ LSSLSKYAED
60 70 80 90 100
IFGELFNEAH SFSFRVNSLQ ERVDRLSVSV TQLDPKEEEL SLQDITMRKA
110 120 130 140 150
FRSSTIQDQQ LFDRKTLPIP LQETYDVCEQ PPPLNVLTPY RDDGKEGLKF
160 170 180 190 200
YTNPSYFFDL WKEKMLQDTE DKRKEKRKQK QKNLDRPHEP EKVPRAPHDR
210 220 230 240 250
RREWQKLAQG PELAEDDADL LHKHIEVANG PASHFETRPQ TYVDHMDGSY
260 270 280 290 300
SLSALPFSQM SELLSRAEER VLVRPHEPPP PPPMHAAGDA RPTPTCVSSA
310 320 330 340 350
AGLIENRPQS PAAGRTPVFV SPTPPPPPPP LPSALSTSSL RASMTSTPPP
360 370 380 390 400
PVPPPPPPPA PALQAPAVPP PPAPLQIAPG VLHPAPPPIA PPLVQPSPPV
410 420 430 440 450
ARAAPVCETV PVHPLPQGEV QGLPPPPPPP PLPPPGIRPS SPVTVAALAH
460 470 480 490 500
PPSGLHPTPS PAPGPHAPLM PPSPPSQVLP ASEPKRHPST LPVISDARSV
510 520 530 540 550
LLEAIRKGIQ LRKVEEQREQ EAKHERIEND VATILSRRIA VEYSDSEDDS

EFDEVDWLE
Length:559
Mass (Da):61,515
Last modified:April 12, 2005 - v1
Checksum:i5682117C6281D401
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC091322 mRNA. Translation: AAH91322.1.
RefSeqiNP_001020285.1. NM_001025114.1.
XP_006256648.1. XM_006256586.2.
UniGeneiRn.7692.

Genome annotation databases

EnsembliENSRNOT00000073091; ENSRNOP00000064342; ENSRNOG00000047476.
GeneIDi294568.
KEGGirno:294568.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC091322 mRNA. Translation: AAH91322.1.
RefSeqiNP_001020285.1. NM_001025114.1.
XP_006256648.1. XM_006256586.2.
UniGeneiRn.7692.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi254741. 3 interactions.
IntActiQ5BJU7. 6 interactions.
MINTiMINT-1795575.

PTM databases

PhosphoSiteiQ5BJU7.

Proteomic databases

PRIDEiQ5BJU7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000073091; ENSRNOP00000064342; ENSRNOG00000047476.
GeneIDi294568.
KEGGirno:294568.

Organism-specific databases

CTDi8936.
RGDi1561954. Wasf1.

Phylogenomic databases

GeneTreeiENSGT00730000110593.
HOVERGENiHBG058482.
InParanoidiQ5BJU7.
KOiK05753.
OMAiQLFDRRT.
OrthoDBiEOG7VHSXK.
PhylomeDBiQ5BJU7.

Miscellaneous databases

NextBioi638305.

Gene expression databases

GenevestigatoriQ5BJU7.

Family and domain databases

InterProiIPR028288. SCAR/WAVE_fam.
IPR003124. WH2_dom.
[Graphical view]
PANTHERiPTHR12902. PTHR12902. 1 hit.
PfamiPF02205. WH2. 1 hit.
[Graphical view]
SMARTiSM00246. WH2. 1 hit.
[Graphical view]
PROSITEiPS51082. WH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  2. "Abelson interacting protein 1 (Abi-1) is essential for dendrite morphogenesis and synapse formation."
    Proepper C., Johannsen S., Liebau S., Dahl J., Vaida B., Bockmann J., Kreutz M.R., Gundelfinger E.D., Boeckers T.M.
    EMBO J. 26:1397-1409(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ABI1, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiWASF1_RAT
AccessioniPrimary (citable) accession number: Q5BJU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 12, 2005
Last modified: January 7, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.