Q5BJU7 (WASF1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Wiskott-Aldrich syndrome protein family member 1
Short name=WASP family protein member 1

Alternative name(s):
Protein WAVE-1

Gene names

Name:	Wasf1
Synonyms:	Wave1

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex By similarity.
Subunit structure	Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterdimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1. Binds actin and the Arp2/3 complex. Interacts with BAIAP2 By similarity.
Subcellular location	Cytoplasm › cytoskeleton By similarity. Note: Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas By similarity.
Domain	Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain By similarity.
Sequence similarities	Belongs to the SCAR/WAVE family. Contains 1 WH2 domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	Actin-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell lamellipodium Inferred from electronic annotation. Source: Compara mitochondrial outer membrane Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 559	559	Wiskott-Aldrich syndrome protein family member 1		PRO_0000314291
Regions
	Domain	497 – 514	18	WH2
	Compositional bias	278 – 283	6	Poly-Pro
	Compositional bias	322 – 332	11	Poly-Pro
	Compositional bias	348 – 361	14	Poly-Pro
	Compositional bias	369 – 374	6	Poly-Pro
	Compositional bias	424 – 435	12	Poly-Pro

Sequences

	Sequence		Length	Mass (Da)
	Q5BJU7 [UniParc]. Last modified April 12, 2005. Version 1. Checksum: 5682117C6281D401	FASTA	559	61,515
	10 20 30 40 50 60 MPLVKRNIDP RHLCHTALPR GIKNELECVT NISLANIIRQ LSSLSKYAED IFGELFNEAH 70 80 90 100 110 120 SFSFRVNSLQ ERVDRLSVSV TQLDPKEEEL SLQDITMRKA FRSSTIQDQQ LFDRKTLPIP 130 140 150 160 170 180 LQETYDVCEQ PPPLNVLTPY RDDGKEGLKF YTNPSYFFDL WKEKMLQDTE DKRKEKRKQK 190 200 210 220 230 240 QKNLDRPHEP EKVPRAPHDR RREWQKLAQG PELAEDDADL LHKHIEVANG PASHFETRPQ 250 260 270 280 290 300 TYVDHMDGSY SLSALPFSQM SELLSRAEER VLVRPHEPPP PPPMHAAGDA RPTPTCVSSA 310 320 330 340 350 360 AGLIENRPQS PAAGRTPVFV SPTPPPPPPP LPSALSTSSL RASMTSTPPP PVPPPPPPPA 370 380 390 400 410 420 PALQAPAVPP PPAPLQIAPG VLHPAPPPIA PPLVQPSPPV ARAAPVCETV PVHPLPQGEV 430 440 450 460 470 480 QGLPPPPPPP PLPPPGIRPS SPVTVAALAH PPSGLHPTPS PAPGPHAPLM PPSPPSQVLP 490 500 510 520 530 540 ASEPKRHPST LPVISDARSV LLEAIRKGIQ LRKVEEQREQ EAKHERIEND VATILSRRIA 550 VEYSDSEDDS EFDEVDWLE

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC091322 mRNA. Translation: AAH91322.1.
IPI	IPI00213598.
RefSeq	NP_001020285.1. NM_001025114.1.
UniGene	Rn.7692.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-1795575.
PTM databases
PhosphoSite	Q5BJU7.
Proteomic databases
PRIDE	Q5BJU7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000073091; ENSRNOP00000064342; ENSRNOG00000047476.
GeneID	294568.
KEGG	rno:294568.
Organism-specific databases
CTD	8936.
RGD	1561954. Wasf1.
Phylogenomic databases
GeneTree	ENSGT00550000074443.
HOVERGEN	HBG058482.
KO	K05753.
Gene expression databases
Genevestigator	Q5BJU7.
Family and domain databases
InterPro	IPR003124. WH2_dom. [Graphical view]
Pfam	PF02205. WH2. 1 hit. [Graphical view]
SMART	SM00246. WH2. 1 hit. [Graphical view]
PROSITE	PS51082. WH2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	638305.

Entry information

Entry name

WASF1_RAT

Accession

Primary (citable) accession number: Q5BJU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 12, 2005
Last modified:	April 3, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program