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Q5BJU7 (WASF1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Wiskott-Aldrich syndrome protein family member 1

Short name=WASP family protein member 1
Alternative name(s):
Protein WAVE-1
Gene names
Name:Wasf1
Synonyms:Wave1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length559 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex By similarity.

Subunit structure

Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterdimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1. Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Interacts with SHANK3; the interaction mediates the association of SHANK3 with the WAVE1 complex. Interacts with ABI1 (via N-terminus). Ref.2

Subcellular location

Cell junctionsynapse. Cytoplasmcytoskeleton By similarity. Note: Dot-like pattern in the cytoplasm. Concentrated in Rac-regulated membrane-ruffling areas By similarity. Ref.2

Tissue specificity

Expressed in hippocampal neurons (at protein level). Ref.2

Domain

Binds the Arp2/3 complex through the C-terminal region and actin through verprolin homology (VPH) domain By similarity.

Sequence similarities

Belongs to the SCAR/WAVE family.

Contains 1 WH2 domain.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 559559Wiskott-Aldrich syndrome protein family member 1
PRO_0000314291

Regions

Domain497 – 51418WH2
Compositional bias278 – 2836Poly-Pro
Compositional bias322 – 33211Poly-Pro
Compositional bias348 – 36114Poly-Pro
Compositional bias369 – 3746Poly-Pro
Compositional bias424 – 43512Poly-Pro

Sequences

Sequence LengthMass (Da)Tools
Q5BJU7 [UniParc].

Last modified April 12, 2005. Version 1.
Checksum: 5682117C6281D401

FASTA55961,515
        10         20         30         40         50         60 
MPLVKRNIDP RHLCHTALPR GIKNELECVT NISLANIIRQ LSSLSKYAED IFGELFNEAH 

        70         80         90        100        110        120 
SFSFRVNSLQ ERVDRLSVSV TQLDPKEEEL SLQDITMRKA FRSSTIQDQQ LFDRKTLPIP 

       130        140        150        160        170        180 
LQETYDVCEQ PPPLNVLTPY RDDGKEGLKF YTNPSYFFDL WKEKMLQDTE DKRKEKRKQK 

       190        200        210        220        230        240 
QKNLDRPHEP EKVPRAPHDR RREWQKLAQG PELAEDDADL LHKHIEVANG PASHFETRPQ 

       250        260        270        280        290        300 
TYVDHMDGSY SLSALPFSQM SELLSRAEER VLVRPHEPPP PPPMHAAGDA RPTPTCVSSA 

       310        320        330        340        350        360 
AGLIENRPQS PAAGRTPVFV SPTPPPPPPP LPSALSTSSL RASMTSTPPP PVPPPPPPPA 

       370        380        390        400        410        420 
PALQAPAVPP PPAPLQIAPG VLHPAPPPIA PPLVQPSPPV ARAAPVCETV PVHPLPQGEV 

       430        440        450        460        470        480 
QGLPPPPPPP PLPPPGIRPS SPVTVAALAH PPSGLHPTPS PAPGPHAPLM PPSPPSQVLP 

       490        500        510        520        530        540 
ASEPKRHPST LPVISDARSV LLEAIRKGIQ LRKVEEQREQ EAKHERIEND VATILSRRIA 

       550 
VEYSDSEDDS EFDEVDWLE 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[2]"Abelson interacting protein 1 (Abi-1) is essential for dendrite morphogenesis and synapse formation."
Proepper C., Johannsen S., Liebau S., Dahl J., Vaida B., Bockmann J., Kreutz M.R., Gundelfinger E.D., Boeckers T.M.
EMBO J. 26:1397-1409(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ABI1, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC091322 mRNA. Translation: AAH91322.1.
RefSeqNP_001020285.1. NM_001025114.1.
XP_006256648.1. XM_006256586.1.
UniGeneRn.7692.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid254741. 3 interactions.
IntActQ5BJU7. 6 interactions.
MINTMINT-1795575.

PTM databases

PhosphoSiteQ5BJU7.

Proteomic databases

PRIDEQ5BJU7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000073091; ENSRNOP00000064342; ENSRNOG00000047476.
GeneID294568.
KEGGrno:294568.

Organism-specific databases

CTD8936.
RGD1561954. Wasf1.

Phylogenomic databases

GeneTreeENSGT00730000110593.
HOVERGENHBG058482.
KOK05753.
OMAYMEHLDG.
OrthoDBEOG7VHSXK.
PhylomeDBQ5BJU7.

Gene expression databases

GenevestigatorQ5BJU7.

Family and domain databases

InterProIPR028288. SCAR/WAVE_fam.
IPR003124. WH2_dom.
[Graphical view]
PANTHERPTHR12902. PTHR12902. 1 hit.
PfamPF02205. WH2. 1 hit.
[Graphical view]
SMARTSM00246. WH2. 1 hit.
[Graphical view]
PROSITEPS51082. WH2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio638305.

Entry information

Entry nameWASF1_RAT
AccessionPrimary (citable) accession number: Q5BJU7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 12, 2005
Last modified: June 11, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families