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Protein

Putative ATP-dependent RNA helicase DHX30

Gene

Dhx30

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the assembly of the mitochondrial large ribosomal subunit (By similarity). Associates with mitochondrial DNA (By similarity). Required for optimal function of the zinc-finger antiviral protein ZC3HAV1 (PubMed:21204022).By similarity1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi457 – 4648ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Ribosome biogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative ATP-dependent RNA helicase DHX30 (EC:3.6.4.13)
Alternative name(s):
DEAH box protein 30
Gene namesi
Name:Dhx30
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi1308888. Dhx30.

Subcellular locationi

  • Mitochondrion matrixmitochondrion nucleoid By similarity
  • Cytoplasm Curated

  • Note: Localizes to mitochondrial RNA granules found in close proximity to the mitochondrial nucleoids.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Mitochondrion nucleoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11941194Putative ATP-dependent RNA helicase DHX30PRO_0000245541Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei6 – 61PhosphoserineBy similarity
Modified residuei226 – 2261PhosphoserineCombined sources
Modified residuei380 – 3801PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5BJS0.
PRIDEiQ5BJS0.

PTM databases

iPTMnetiQ5BJS0.
PhosphoSiteiQ5BJS0.

Expressioni

Gene expression databases

ExpressionAtlasiQ5BJS0. baseline and differential.
GenevisibleiQ5BJS0. RN.

Interactioni

Subunit structurei

Identified in a complex with TFAM and SSBP1 (By similarity). Interacts (via N-terminus) with ZC3HAV1 (via N-terminal domain) in an RNA-independent manner. Found in a complex with GRSF1, DDX28, FASTKD2 and FASTKD5 (By similarity).By similarity1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043896.

Structurei

3D structure databases

ProteinModelPortaliQ5BJS0.
SMRiQ5BJS0. Positions 44-152.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini53 – 12169DRBMAdd
BLAST
Domaini444 – 612169Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini654 – 827174Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi559 – 5624DEAH box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi188 – 19912Poly-GluAdd
BLAST
Compositional biasi1010 – 10134Poly-Glu

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0920. Eukaryota.
COG1643. LUCA.
GeneTreeiENSGT00760000119189.
HOGENOMiHOG000112212.
HOVERGENiHBG081437.
InParanoidiQ5BJS0.
KOiK13185.
OMAiPLTHAMY.
OrthoDBiEOG7JX33D.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5BJS0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFTLDSFRKD RTQHRQRQCK LPPPRLPPMC VNPAPGGTIS RASRDLLKEF
60 70 80 90 100
PQPKNLLNSV IGRALGISHA KDKLVYVHTN GPKKKKVTLH IKWPKSVEVE
110 120 130 140 150
GYGSKKIDAE RQAAAAACQL FKGWGLLGPR NELFDAAKYR VLADRFGSPA
160 170 180 190 200
DSWWRPEPTM PPTSWRQLNP ENIRPAGTGG LSRSLGREEE EDEEEELEEG
210 220 230 240 250
TIDVTEFLSM TQQDSHNPLR DSRGGSFEMT DDDSAIRALT QFPLPKNLLA
260 270 280 290 300
KVIQIATSSS TAKNLMQFHT VGTKTKLATL TLLWPCPMTF VAKGRRKAEA
310 320 330 340 350
ENKAAALACK KLKSLGLVDR NNEPLTHAMY NLASLRELGE TQRRPCTIQV
360 370 380 390 400
PEPILRKIEA FLSHYPVDSS WISPELRLQS DDILPLGKDS GPLSDPITGK
410 420 430 440 450
PYMPLSEAEE VRLSQSLLEL WRRRGPIWQE APQLPVDPHR DTILSAIEQH
460 470 480 490 500
PVVVISGDTG CGKTTRIPQL LLERYVTEGR GARCNVIITQ PRRISAVSVA
510 520 530 540 550
QRVSHELGPS LRRNVGFQVR LESKPPARGG ALLFCTVGIL LRKLQSNPSL
560 570 580 590 600
EGVSHVIVDE VHERDVNTDF LLILLKGLQR LNPALRLVLM SATGDNERFS
610 620 630 640 650
RYFGGCPVIK VPGFMYPVKE HYLEDILAKL GKHQYPHRHR HHESEDECAL
660 670 680 690 700
DLDLVTDLVL HIDARGEPGG ILCFLPGWQE IKGVQQRLQE ALGMHESKYL
710 720 730 740 750
ILPVHSNIPM MDQKAIFQQP PLGVRKIVLA TNIAETSITV NDIVHVVDSG
760 770 780 790 800
LHKEERYDLK TKVSCLETVW VSRANVIQRR GRAGRCQSGF AYHLFPRSRL
810 820 830 840 850
EKMVPFQVPE ILRTPLENLV LQAKIHMPEK TAVEFLSKAV DSPNIKAVDE
860 870 880 890 900
AVILLQEIGV LDQREYLTTL GQRLAHISTD PRLAKAIVLA AIFRCLHPLL
910 920 930 940 950
VVVSCLTRDP FSSSLQNRAE VDKVKALLSH DSGSDHLAFV RAVAGWEEVL
960 970 980 990 1000
RWQDRTSREN YLEENLLYAP SLRFIHGLIK QFSENIYEAF LVGKPSDCTL
1010 1020 1030 1040 1050
PSAQCNEYSE EEELVKGVLM AGLYPNLIQV RQGKVTRQGK FKPNSVTYRT
1060 1070 1080 1090 1100
KSGNILLHKS TINREATRLR SRWLTYFMAV KSNGSVFVRD SSQVHPLAVL
1110 1120 1130 1140 1150
LLTDGDVHIR DDGRRATISL SDSDLLRLEG DSRTVRLLRE LRRALGRMVE
1160 1170 1180 1190
RSLRSELAAL PLSVQQEHGQ LLALLAELLR GPCGSFDVRK TADD
Length:1,194
Mass (Da):133,997
Last modified:April 12, 2005 - v1
Checksum:iA453E243EB1A5929
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC091359 mRNA. Translation: AAH91359.1.
RefSeqiNP_001013267.1. NM_001013249.1.
UniGeneiRn.163034.

Genome annotation databases

EnsembliENSRNOT00000048764; ENSRNOP00000043896; ENSRNOG00000029194.
GeneIDi367172.
KEGGirno:367172.
UCSCiRGD:1308888. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC091359 mRNA. Translation: AAH91359.1.
RefSeqiNP_001013267.1. NM_001013249.1.
UniGeneiRn.163034.

3D structure databases

ProteinModelPortaliQ5BJS0.
SMRiQ5BJS0. Positions 44-152.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000043896.

PTM databases

iPTMnetiQ5BJS0.
PhosphoSiteiQ5BJS0.

Proteomic databases

PaxDbiQ5BJS0.
PRIDEiQ5BJS0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000048764; ENSRNOP00000043896; ENSRNOG00000029194.
GeneIDi367172.
KEGGirno:367172.
UCSCiRGD:1308888. rat.

Organism-specific databases

CTDi22907.
RGDi1308888. Dhx30.

Phylogenomic databases

eggNOGiKOG0920. Eukaryota.
COG1643. LUCA.
GeneTreeiENSGT00760000119189.
HOGENOMiHOG000112212.
HOVERGENiHBG081437.
InParanoidiQ5BJS0.
KOiK13185.
OMAiPLTHAMY.
OrthoDBiEOG7JX33D.

Miscellaneous databases

PROiQ5BJS0.

Gene expression databases

ExpressionAtlasiQ5BJS0. baseline and differential.
GenevisibleiQ5BJS0. RN.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  2. "DEXH-Box protein DHX30 is required for optimal function of the zinc-finger antiviral protein."
    Ye P., Liu S., Zhu Y., Chen G., Gao G.
    Protein Cell 1:956-964(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH ZC3HAV1.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-226, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDHX30_RAT
AccessioniPrimary (citable) accession number: Q5BJS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: April 12, 2005
Last modified: July 6, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.