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Protein

Atypical kinase COQ8A, mitochondrial

Gene

Coq8a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Atypical kinase involved in the biosynthesis of coenzyme Q, also named ubiquinone, an essential lipid-soluble electron transporter for aerobic cellular respiration. Its substrate specificity is unclear: does not show any protein kinase activity. Probably acts as a small molecule kinase, possibly a lipid kinase that phosphorylates a prenyl lipid in the ubiquinone biosynthesis pathway, as suggested by its ability to bind coenzyme Q lipid intermediates. Shows an unusual selectivity for binding ADP over ATP.By similarity

Enzyme regulationi

Autoinhibited by the N-terminal domain, containing the KxGQ motif, that completely occludes the typical substrate binding pocket. Nucleotide-binding relieves inhibition.By similarity

Pathwayi: ubiquinone biosynthesis

This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei341ATPBy similarity1
Binding sitei359ATPBy similarity1
Active sitei489Proton acceptorBy similarity1
Binding sitei494ATPBy similarity1
Binding sitei508ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi446 – 449ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Ubiquinone biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00232.

Names & Taxonomyi

Protein namesi
Recommended name:
Atypical kinase COQ8A, mitochondrialCurated (EC:2.7.-.-By similarity)
Alternative name(s):
Chaperone activity of bc1 complex-likeBy similarity
Short name:
Chaperone-ABC1-likeBy similarity
Coenzyme Q protein 8ABy similarity
aarF domain-containing protein kinase 3
Gene namesi
Name:Coq8aBy similarity
Synonyms:Adck3Imported, Cabc1By similarity
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi1308245. Adck3.

Subcellular locationi

  • Mitochondrion By similarity
  • Membrane Sequence analysis; Single-pass membrane protein Sequence analysisBy similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei215 – 231HelicalSequence analysisAdd BLAST17

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 163MitochondrionBy similarityAdd BLAST163
ChainiPRO_0000271795164 – 649Atypical kinase COQ8A, mitochondrialAdd BLAST486

Proteomic databases

PaxDbiQ5BJQ0.
PRIDEiQ5BJQ0.

PTM databases

iPTMnetiQ5BJQ0.
PhosphoSitePlusiQ5BJQ0.

Expressioni

Gene expression databases

BgeeiENSRNOG00000043201.
GenevisibleiQ5BJQ0. RN.

Interactioni

Subunit structurei

Homodimer; homodimerizes via its transmembrane region. Interacts with the multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000036948.

Structurei

3D structure databases

ProteinModelPortaliQ5BJQ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini330 – 519Protein kinaseAdd BLAST190

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi277 – 280KxGQ motifBy similarity4
Motifi338 – 341AAAS motifBy similarity4

Domaini

Adopts an atypical protein kinase-like fold: while it adopts a core fold similar to that of well-characterized protein kinase-like domains, a number of features are positioned to inhibit the kinase activity: (1) an atypical AAAS motif in an alanine-rich (A-rich) loop that replaces the canonical glycine-rich (G-rich) nucleotide-binding loop and limits ATP binding by establishing an unusual selectivity for ADP and (2) an N-terminal domain, containing the KxGQ motif, that completely occludes the typical substrate binding pocket. Nucleotide-binding opens the substrate binding pocket and flips the active site from inside the hydrophobic core into a catalytically competent, solvent-exposed posture.By similarity

Sequence similaritiesi

Contains 1 protein kinase domain.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1234. Eukaryota.
COG0661. LUCA.
GeneTreeiENSGT00550000074739.
HOGENOMiHOG000201140.
HOVERGENiHBG061318.
InParanoidiQ5BJQ0.
KOiK08869.
OMAiYEVKVME.
PhylomeDBiQ5BJQ0.
TreeFamiTF300630.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR004147. UbiB_dom.
[Graphical view]
PfamiPF03109. ABC1. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5BJQ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAMLGDAIM VAKGLAKLTQ AAVETHLQNL GLGGELILAA RALQSTAVEQ
60 70 80 90 100
ISMVFGKVQG QDKHEDSYAT ENFEDLEAEV QFSTPQAAGS SPDFSTASSL
110 120 130 140 150
DQPLSSSLGH AHREGPAPAY VSSGPFREAG LSGQATSPLG RVNGRLFVDC
160 170 180 190 200
RDLFLANSIQ RRFFHQDQAP VGGLTAEDIE KARQAKARPE SKPHKQMLSE
210 220 230 240 250
RARERKVPVT RIGRLANFGG LAVGLGFGAL AEVAKKSLRS ENSTGKKAVL
260 270 280 290 300
DSSPFLSEAN AERIVSTLCK VRGAALKLGQ MLSIQDDAFI NPHLAKIFER
310 320 330 340 350
VRQSADFMPL KQMTKTLNND LGPHWRDKLE YFEERPFAAA SIGQVHLARL
360 370 380 390 400
KGGREVAMKI QYPGVAQSIN SDVNNLMAVL NMSNMLPEGL FPEHLIDVLR
410 420 430 440 450
RELTLECDYQ REAAYAKKFR ELLKDHPFFY VPEIVDELCS PHVLTTELIT
460 470 480 490 500
GFPLDQAEGL SQEVRNEICY NILVLCLREL FEFHVMQTDP NWSNFFYDPQ
510 520 530 540 550
QHKVALLDFG ATREYDRSFT DLYIQVIRAA ADQDREAVLK KSIEMKFLTG
560 570 580 590 600
YEVKAMEDAH LDAILILGEA FASEEPFDFG TQSTTEKIHN LIPIMLKHRL
610 620 630 640
IPPPEETYSL HRKMGGSFLI CSKLKACFPC KAMFEEAYSN YCRMKSGLQ
Length:649
Mass (Da):72,226
Last modified:April 12, 2005 - v1
Checksum:iE7EBC66B6163A9F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC091388 mRNA. Translation: AAH91388.1.
RefSeqiNP_001013203.1. NM_001013185.1.
XP_006250449.1. XM_006250387.1.
UniGeneiRn.160865.

Genome annotation databases

EnsembliENSRNOT00000030135; ENSRNOP00000036948; ENSRNOG00000043201.
ENSRNOT00000086306; ENSRNOP00000070234; ENSRNOG00000043201.
GeneIDi360887.
KEGGirno:360887.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC091388 mRNA. Translation: AAH91388.1.
RefSeqiNP_001013203.1. NM_001013185.1.
XP_006250449.1. XM_006250387.1.
UniGeneiRn.160865.

3D structure databases

ProteinModelPortaliQ5BJQ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000036948.

PTM databases

iPTMnetiQ5BJQ0.
PhosphoSitePlusiQ5BJQ0.

Proteomic databases

PaxDbiQ5BJQ0.
PRIDEiQ5BJQ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000030135; ENSRNOP00000036948; ENSRNOG00000043201.
ENSRNOT00000086306; ENSRNOP00000070234; ENSRNOG00000043201.
GeneIDi360887.
KEGGirno:360887.

Organism-specific databases

CTDi360887.
RGDi1308245. Adck3.

Phylogenomic databases

eggNOGiKOG1234. Eukaryota.
COG0661. LUCA.
GeneTreeiENSGT00550000074739.
HOGENOMiHOG000201140.
HOVERGENiHBG061318.
InParanoidiQ5BJQ0.
KOiK08869.
OMAiYEVKVME.
PhylomeDBiQ5BJQ0.
TreeFamiTF300630.

Enzyme and pathway databases

UniPathwayiUPA00232.

Miscellaneous databases

PROiQ5BJQ0.

Gene expression databases

BgeeiENSRNOG00000043201.
GenevisibleiQ5BJQ0. RN.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR004147. UbiB_dom.
[Graphical view]
PfamiPF03109. ABC1. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiCOQ8A_RAT
AccessioniPrimary (citable) accession number: Q5BJQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: April 12, 2005
Last modified: November 2, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.