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Protein

Transmembrane protein 97

Gene

TMEM97

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role as a regulator of cellular cholesterol homeostasis (PubMed:19583955). May function as sterol isomerase (PubMed:25566323).1 Publication1 Publication

GO - Biological processi

  • cholesterol homeostasis Source: UniProtKB
  • regulation of cell growth Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane protein 97
Alternative name(s):
Meningioma-associated protein 301 Publication
Gene namesi
Name:TMEM97
Synonyms:MAC30
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:28106. TMEM97.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicSequence analysis
Transmembranei10 – 3021Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini31 – 6838ExtracellularSequence analysisAdd
BLAST
Transmembranei69 – 8921Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini90 – 9910CytoplasmicSequence analysis
Transmembranei100 – 12021Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini121 – 14020ExtracellularSequence analysisAdd
BLAST
Transmembranei141 – 16121Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini162 – 17615CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • lysosome Source: UniProtKB
  • nuclear membrane Source: UniProtKB
  • plasma membrane Source: UniProtKB
  • rough endoplasmic reticulum Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Lysosome, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA143485635.

Polymorphism and mutation databases

BioMutaiTMEM97.
DMDMi74736009.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 176176Transmembrane protein 97PRO_0000254567Add
BLAST

Proteomic databases

EPDiQ5BJF2.
MaxQBiQ5BJF2.
PaxDbiQ5BJF2.
PRIDEiQ5BJF2.
TopDownProteomicsiQ5BJF2.

PTM databases

iPTMnetiQ5BJF2.
PhosphoSiteiQ5BJF2.
SwissPalmiQ5BJF2.

Expressioni

Tissue specificityi

Widely expressed in normal tissues. Expressed in pancreatic, renal, breast, colon, ovarian surface epithelial (OSE) cells and meningioma cancers. Expressed in ovarian cancer; expression is reduced relative to OSE cells.3 Publications

Inductioni

Up-regulated in ovarian surface epithelial (OSE) cells with progesterone.1 Publication

Gene expression databases

BgeeiQ5BJF2.
CleanExiHS_TMEM97.
ExpressionAtlasiQ5BJF2. baseline and differential.
GenevisibleiQ5BJF2. HS.

Organism-specific databases

HPAiHPA044795.

Interactioni

Subunit structurei

Interacts with NPC1.1 Publication

Protein-protein interaction databases

BioGridi118158. 10 interactions.
STRINGi9606.ENSP00000226230.

Structurei

3D structure databases

ProteinModelPortaliQ5BJF2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 158149EXPERAPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TMEM97 family.Curated
Contains 1 EXPERA domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IX1Z. Eukaryota.
ENOG4111RR6. LUCA.
GeneTreeiENSGT00390000007149.
HOGENOMiHOG000031140.
HOVERGENiHBG079913.
InParanoidiQ5BJF2.
OMAiFKGGCKW.
PhylomeDBiQ5BJF2.
TreeFamiTF300241.

Family and domain databases

InterProiIPR033118. EXPERA.
IPR016964. Transmembrane_97.
[Graphical view]
PIRSFiPIRSF031032. TMP_97_prd. 1 hit.
PROSITEiPS51751. EXPERA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5BJF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAPATRRCV EWLLGLYFLS HIPITLFMDL QAVLPRELYP VEFRNLLKWY
60 70 80 90 100
AKEFKDPLLQ EPPAWFKSFL FCELVFQLPF FPIATYAFLK GSCKWIRTPA
110 120 130 140 150
IIYSVHTMTT LIPILSTFLF EDFSKASGFK GQRPETLHER LTLVSVYAPY
160 170
LLIPFILLIF MLRSPYYKYE EKRKKK
Length:176
Mass (Da):20,848
Last modified:April 12, 2005 - v1
Checksum:i685248D421877F84
GO

Sequence cautioni

The sequence AAA16188.1 differs from that shown. Reason: Frameshift at position 5. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51A → S in AAA16188 (PubMed:7694637).Curated
Sequence conflicti34 – 341L → V in AAA16188 (PubMed:7694637).Curated
Sequence conflicti113 – 1131P → L in AAA16188 (PubMed:7694637).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19183 mRNA. Translation: AAA16188.1. Frameshift.
AK299511 mRNA. Translation: BAG61464.1.
AC002094 Genomic DNA. No translation available.
BC091504 mRNA. Translation: AAH91504.1.
CCDSiCCDS11226.2.
PIRiI65744.
RefSeqiNP_055388.2. NM_014573.2.
UniGeneiHs.199695.

Genome annotation databases

EnsembliENST00000226230; ENSP00000226230; ENSG00000109084.
GeneIDi27346.
KEGGihsa:27346.
UCSCiuc002hat.3. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19183 mRNA. Translation: AAA16188.1. Frameshift.
AK299511 mRNA. Translation: BAG61464.1.
AC002094 Genomic DNA. No translation available.
BC091504 mRNA. Translation: AAH91504.1.
CCDSiCCDS11226.2.
PIRiI65744.
RefSeqiNP_055388.2. NM_014573.2.
UniGeneiHs.199695.

3D structure databases

ProteinModelPortaliQ5BJF2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi118158. 10 interactions.
STRINGi9606.ENSP00000226230.

PTM databases

iPTMnetiQ5BJF2.
PhosphoSiteiQ5BJF2.
SwissPalmiQ5BJF2.

Polymorphism and mutation databases

BioMutaiTMEM97.
DMDMi74736009.

Proteomic databases

EPDiQ5BJF2.
MaxQBiQ5BJF2.
PaxDbiQ5BJF2.
PRIDEiQ5BJF2.
TopDownProteomicsiQ5BJF2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000226230; ENSP00000226230; ENSG00000109084.
GeneIDi27346.
KEGGihsa:27346.
UCSCiuc002hat.3. human.

Organism-specific databases

CTDi27346.
GeneCardsiTMEM97.
HGNCiHGNC:28106. TMEM97.
HPAiHPA044795.
MIMi612912. gene.
neXtProtiNX_Q5BJF2.
PharmGKBiPA143485635.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IX1Z. Eukaryota.
ENOG4111RR6. LUCA.
GeneTreeiENSGT00390000007149.
HOGENOMiHOG000031140.
HOVERGENiHBG079913.
InParanoidiQ5BJF2.
OMAiFKGGCKW.
PhylomeDBiQ5BJF2.
TreeFamiTF300241.

Miscellaneous databases

ChiTaRSiTMEM97. human.
GenomeRNAii27346.
NextBioi50434.
PROiQ5BJF2.
SOURCEiSearch...

Gene expression databases

BgeeiQ5BJF2.
CleanExiHS_TMEM97.
ExpressionAtlasiQ5BJF2. baseline and differential.
GenevisibleiQ5BJF2. HS.

Family and domain databases

InterProiIPR033118. EXPERA.
IPR016964. Transmembrane_97.
[Graphical view]
PIRSFiPIRSF031032. TMP_97_prd. 1 hit.
PROSITEiPS51751. EXPERA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of genes differentially expressed in meningiomas."
    Murphy M., Pykett M.J., Harnish P., Zang K.D., George D.L.
    Cell Growth Differ. 4:715-722(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  5. "Expression analysis of MAC30 in human pancreatic cancer and tumors of the gastrointestinal tract."
    Kayed H., Kleeff J., Ding J., Hammer J., Giese T., Zentgraf H., Buchler M.W., Friess H.
    Histol. Histopathol. 19:1021-1031(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "Coordinate up-regulation of TMEM97 and cholesterol biosynthesis genes in normal ovarian surface epithelial cells treated with progesterone: implications for pathogenesis of ovarian cancer."
    Wilcox C.B., Feddes G.O., Willett-Brozick J.E., Hsu L.C., DeLoia J.A., Baysal B.E.
    BMC Cancer 7:223-223(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  7. "Identification of cholesterol-regulating genes by targeted RNAi screening."
    Bartz F., Kern L., Erz D., Zhu M., Gilbert D., Meinhof T., Wirkner U., Erfle H., Muckenthaler M., Pepperkok R., Runz H.
    Cell Metab. 10:63-75(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NPC1, SUBCELLULAR LOCATION.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "TM6SF2 and MAC30, new enzyme homologs in sterol metabolism and common metabolic disease."
    Sanchez-Pulido L., Ponting C.P.
    Front. Genet. 5:439-439(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE FUNCTION AS STEROL ISOMERASE, IDENTIFICATION OF EXPERA DOMAIN.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiTMM97_HUMAN
AccessioniPrimary (citable) accession number: Q5BJF2
Secondary accession number(s): B4DS02, Q07823
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: April 12, 2005
Last modified: March 16, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.