Reviewed, UniProtKB/Swiss-Prot Q5BJ68 (NB5R2_XENTR)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase 2
      Short name=b5R.2
    EC=1.6.2.2

Gene names

Name:	cyb5r2
ORF Names:	TNeu132f07.1

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis and drug metabolism By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	NADH-cytochrome b5 reductase 2		PRO_0000287554
Regions
	Transmembrane	9 – 29	21	Potential
	Domain	43 – 155	113	FAD-binding FR-type
	Nucleotide binding	135 – 150	16	FAD By similarity
	Nucleotide binding	174 – 209	36	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5BJ68-1 [UniParc]. Last modified April 12, 2005. Version 1. Checksum: 0790DEB00965A0A6	FASTA	304	33,745
	10 20 30 40 50 60 MEISTDSNML VALAVIGVTV LLFLIKALGS QAKKAPLTLL DPNAKYPLPL IEKQEISHDT 70 80 90 100 110 120 KKFRFGLPSQ EHVLGLPVGQ HVYLSAKING SLVVRAYTPV SSDEVKGHVD LIVKVYYKNV 130 140 150 160 170 180 HPKFPEGGKM SQHLDSLKIG ETIDFRGPNG LLVYKEKGKF AIRPDKKSEP KLKVAKHVGM 190 200 210 220 230 240 LAGGTGITPM LQLIRQITQD PNDNTKCSLI FANQTEDDIL LRYELETVAK SHPEQFKLWY 250 260 270 280 290 300 TLDRPPQGWK YGAGFVTADM IKEHLPPPSE DVVVLMCGPP PMIQFACQDN LTKLGYPEAG RFAY

References

[1]	Sanger Xenopus tropicalis EST/cDNA project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Neurula.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR942741 mRNA. Translation: CAJ83022.1. BC091602 mRNA. Translation: AAH91602.1.
RefSeq	NP_001025638.1.
UniGene	Str.5754
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSXETT00000019844; ENSXETP00000019844; ENSXETG00000009054; Xenopus tropicalis. [Genome view]
GeneID	595026.
KEGG	xtr:595026.
Organism-specific databases
CTD	595026.
Xenbase	XB-FEAT-1006040. cyb5r2.
Phylogenomic databases
HOGENOM	Q5BJ68.
HOVERGEN	Q5BJ68.
OMA	EWSYSSG.
Enzyme and pathway databases
BRENDA	1.6.2.2. 279072.
Gene expression databases
Bgee	Q5BJ68.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NB5R2_XENTR

Accession

Primary (citable) accession number: Q5BJ68

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	April 12, 2005
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project