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Protein

Dual specificity protein phosphatase 18

Gene

DUSP18

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine.By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei104 – 1041Phosphocysteine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. MAP kinase tyrosine/serine/threonine phosphatase activity Source: InterPro
  2. protein tyrosine/serine/threonine phosphatase activity Source: GO_Central
  3. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. peptidyl-tyrosine dephosphorylation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 18 (EC:3.1.3.16, EC:3.1.3.48)
Gene namesi
Name:DUSP18
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136 Componenti: Chromosome 17

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity. Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity; Intermembrane side By similarity
Note: Translocates to cytoplasm in response to apoptotic stimuli such as staurosporine treatment.By similarity

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 188188Dual specificity protein phosphatase 18PRO_0000094827Add
BLAST

Proteomic databases

PRIDEiQ5BIP9.

Structurei

3D structure databases

ProteinModelPortaliQ5BIP9.
SMRiQ5BIP9. Positions 18-179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 14970Tyrosine-protein phosphataseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni95 – 14147Sufficient for mitochondrial localizationBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2453.
GeneTreeiENSGT00760000118853.
HOGENOMiHOG000233766.
HOVERGENiHBG051422.
InParanoidiQ5BIP9.
KOiK14165.
OMAiASPCAFP.
OrthoDBiEOG7PK90H.
TreeFamiTF316009.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR020417. Atypical_DUSP.
IPR020420. Atypical_DUSP_famB.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
PRINTSiPR01908. ADSPHPHTASE.
PR01910. ADSPHPHTASEB.
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5BIP9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTASPCAFPV QFRQPSVSGL SQITSSLYIS SGVAANNRLM LSSNRISTVI
60 70 80 90 100
NVSVEVVNAL YEDIHYVQVP VADTPTSRLC DFFDPIADHI HSVEMKQGRT
110 120 130 140 150
LLHCAAGVSR SAALCLAYLM KYHAMSLLDA HTWTKSCRPI IRPNNGFWEQ
160 170 180
LIHYEFQLFG RNTVHMVSSP VGMIPDIYEK EVRQMIPL
Length:188
Mass (Da):21,028
Last modified:April 12, 2005 - v1
Checksum:i3EADB96A25E1CE6B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti31 – 311S → R in AAI10014 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021175 mRNA. Translation: AAX31357.1.
BC110013 mRNA. Translation: AAI10014.1.
RefSeqiNP_001029431.1. NM_001034259.1.
UniGeneiBt.37370.

Genome annotation databases

EnsembliENSBTAT00000023205; ENSBTAP00000023205; ENSBTAG00000017459.
GeneIDi505912.
KEGGibta:505912.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021175 mRNA. Translation: AAX31357.1.
BC110013 mRNA. Translation: AAI10014.1.
RefSeqiNP_001029431.1. NM_001034259.1.
UniGeneiBt.37370.

3D structure databases

ProteinModelPortaliQ5BIP9.
SMRiQ5BIP9. Positions 18-179.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ5BIP9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000023205; ENSBTAP00000023205; ENSBTAG00000017459.
GeneIDi505912.
KEGGibta:505912.

Organism-specific databases

CTDi150290.

Phylogenomic databases

eggNOGiCOG2453.
GeneTreeiENSGT00760000118853.
HOGENOMiHOG000233766.
HOVERGENiHBG051422.
InParanoidiQ5BIP9.
KOiK14165.
OMAiASPCAFP.
OrthoDBiEOG7PK90H.
TreeFamiTF316009.

Miscellaneous databases

NextBioi20867370.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR020417. Atypical_DUSP.
IPR020420. Atypical_DUSP_famB.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
PRINTSiPR01908. ADSPHPHTASE.
PR01910. ADSPHPHTASEB.
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.

Entry informationi

Entry nameiDUS18_BOVIN
AccessioniPrimary (citable) accession number: Q5BIP9
Secondary accession number(s): Q32KN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: April 12, 2005
Last modified: February 4, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.