Reviewed, UniProtKB/Swiss-Prot Q5BIP9 (DUS18_BOVIN)

Names and origin

Protein names

Recommended name:
    Dual specificity protein phosphatase 18
    EC=3.1.3.48
    EC=3.1.3.16

Gene names

Name:

DUSP18

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphoprotein + H2O = a protein + phosphate.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Molecular function	Hydrolase Protein phosphatase
Gene Ontology (GO)
Biological process	protein amino acid dephosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	MAP kinase tyrosine/serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Dual specificity protein phosphatase 18		PRO_0000094827
Regions
	Domain	80 – 149	70	Tyrosine-protein phosphatase
Sites
	Active site	104	1	Phosphocysteine intermediate By similarity
Experimental info
	Sequence conflict	31	1	S → R in AAI10014. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q5BIP9-1 [UniParc]. Last modified April 12, 2005. Version 1. Checksum: 3EADB96A25E1CE6B	FASTA	188	21,028
	10 20 30 40 50 60 MTASPCAFPV QFRQPSVSGL SQITSSLYIS SGVAANNRLM LSSNRISTVI NVSVEVVNAL 70 80 90 100 110 120 YEDIHYVQVP VADTPTSRLC DFFDPIADHI HSVEMKQGRT LLHCAAGVSR SAALCLAYLM 130 140 150 160 170 180 KYHAMSLLDA HTWTKSCRPI IRPNNGFWEQ LIHYEFQLFG RNTVHMVSSP VGMIPDIYEK EVRQMIPL

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT021175 mRNA. Translation: AAX31357.1. BC110013 mRNA. Translation: AAI10014.1.
IPI	IPI00716808.
RefSeq	NP_001029431.1.
UniGene	Bt.37370
3D structure databases
SMR	Q5BIP9. Positions 18-179.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000023205; ENSBTAP00000023205; ENSBTAG00000017459; Bos taurus. [Genome view]
GeneID	505912.
KEGG	bta:505912.
Organism-specific databases
CTD	505912.
Phylogenomic databases
eggNOG	maNOG07361.
HOVERGEN	Q5BIP9.
InParanoid	Q5BIP9.
OMA	MGVIPDI.
OrthoDB	EOG90043R.
PhylomeDB	Q5BIP9.
Enzyme and pathway databases
BRENDA	3.1.3.16. 251. 3.1.3.48. 251.
Family and domain databases
InterPro	IPR000387. Dual-sp/Tyr_phosphatase. IPR020417. Dual-sp_phosphatase. IPR000340. Dual-sp_phosphatase_cat-dom. IPR020420. Dual-sp_phosphatase_famB. IPR020422. Dual-sp_phosphatase_subgr_cat. IPR016130. Tyr_Pase_AS. [Graphical view]
Pfam	PF00782. DSPc. 1 hit. [Graphical view]
PRINTS	PR01908. ADSPHPHTASE. PR01910. ADSPHPHTASEB.
SMART	SM00195. DSPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50054. TYR_PHOSPHATASE_DUAL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DUS18_BOVIN

Accession

Primary (citable) accession number: Q5BIP9
Secondary accession number(s): Q32KN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	April 12, 2005
Last modified:	January 19, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)