Q5BIP9 (DUS18_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dual specificity protein phosphatase 18
EC=3.1.3.16
EC=3.1.3.48

Gene names

Name:

DUSP18

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	188 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphoprotein + H2O = a protein + phosphate.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side By similarity. Note: Translocates to cytoplasm in response to apoptotic stimuli such as staurosporine treatment By similarity.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane Mitochondrion Mitochondrion inner membrane Nucleus
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine dephosphorylation Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	MAP kinase tyrosine/serine/threonine phosphatase activity Inferred from electronic annotation. Source: InterPro protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC protein tyrosine/serine/threonine phosphatase activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 188	188	Dual specificity protein phosphatase 18		PRO_0000094827
Regions
	Domain	80 – 149	70	Tyrosine-protein phosphatase
	Region	95 – 141	47	Sufficient for mitochondrial localization By similarity
Sites
	Active site	104	1	Phosphocysteine intermediate By similarity
Experimental info
	Sequence conflict	31	1	S → R in AAI10014. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q5BIP9 [UniParc]. Last modified April 12, 2005. Version 1. Checksum: 3EADB96A25E1CE6B	FASTA	188	21,028
	10 20 30 40 50 60 MTASPCAFPV QFRQPSVSGL SQITSSLYIS SGVAANNRLM LSSNRISTVI NVSVEVVNAL 70 80 90 100 110 120 YEDIHYVQVP VADTPTSRLC DFFDPIADHI HSVEMKQGRT LLHCAAGVSR SAALCLAYLM 130 140 150 160 170 180 KYHAMSLLDA HTWTKSCRPI IRPNNGFWEQ LIHYEFQLFG RNTVHMVSSP VGMIPDIYEK EVRQMIPL

References

[1]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (NOV-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Crossbred X Angus. Tissue: Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT021175 mRNA. Translation: AAX31357.1. BC110013 mRNA. Translation: AAI10014.1.
IPI	IPI00716808.
RefSeq	NP_001029431.1. NM_001034259.1.
UniGene	Bt.37370.
3D structure databases
ProteinModelPortal	Q5BIP9.
SMR	Q5BIP9. Positions 18-179.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000023205; ENSBTAP00000023205; ENSBTAG00000017459.
GeneID	505912.
KEGG	bta:505912.
Organism-specific databases
CTD	150290.
Phylogenomic databases
eggNOG	COG2453.
GeneTree	ENSGT00700000104026.
HOGENOM	HOG000233766.
HOVERGEN	HBG051422.
InParanoid	Q5BIP9.
KO	K14165.
OMA	AMEDFYQ.
OrthoDB	EOG4VQ9QB.
Family and domain databases
InterPro	IPR020417. Atypical_DUSP. IPR020420. Atypical_DUSP_famB. IPR000340. Dual-sp_phosphatase_cat-dom. IPR020422. Dual-sp_phosphatase_subgr_cat. IPR024950. DUSP. IPR000387. Tyr/Dual-sp_Pase. IPR016130. Tyr_Pase_AS. [Graphical view]
PANTHER	PTHR10159. PTHR10159. 1 hit.
Pfam	PF00782. DSPc. 1 hit. [Graphical view]
PRINTS	PR01908. ADSPHPHTASE. PR01910. ADSPHPHTASEB.
SMART	SM00195. DSPc. 1 hit. [Graphical view]
PROSITE	PS00383. TYR_PHOSPHATASE_1. 1 hit. PS50056. TYR_PHOSPHATASE_2. 1 hit. PS50054. TYR_PHOSPHATASE_DUAL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20867370.

Entry information

Entry name

DUS18_BOVIN

Accession

Primary (citable) accession number: Q5BIP9
Secondary accession number(s): Q32KN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	April 12, 2005
Last modified:	March 6, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program