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Q5BIP7

- LIAS_BOVIN

UniProt

Q5BIP7 - LIAS_BOVIN

Protein

Lipoyl synthase, mitochondrial

Gene

LIAS

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (12 Apr 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi106 – 1061Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi111 – 1111Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi117 – 1171Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi137 – 1371Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi141 – 1411Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi144 – 1441Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. inflammatory response Source: Ensembl
    2. neural tube closure Source: Ensembl
    3. protein lipoylation Source: UniProtKB-HAMAP
    4. response to lipopolysaccharide Source: Ensembl
    5. response to oxidative stress Source: Ensembl

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    Name:LIASUniRule annotation
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 6

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2727MitochondrionUniRule annotationAdd
    BLAST
    Chaini28 – 372345Lipoyl synthase, mitochondrialPRO_0000332310Add
    BLAST

    Proteomic databases

    PRIDEiQ5BIP7.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5BIP7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0320.
    GeneTreeiENSGT00390000006234.
    HOGENOMiHOG000235998.
    HOVERGENiHBG023328.
    InParanoidiQ5BIP7.
    KOiK03644.
    OMAiPEEPYNT.
    TreeFamiTF300817.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5BIP7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLRCGGAVR TVGPRVFGRY VFSPVREVSF LPDEKKEFLQ SGPDLQEFIS    50
    GNLADKSTWD EYKGNLKRQK GERLRLPPWL KTEIPMGKNY NKLKNTLRNL 100
    NLHTVCEEAR CPNIGECWGG GEYATATATI MLMGDTCTRG CRFCSVKTAR 150
    NPPPLDANEP YNTAKAIAEW GLDYVVLTSV DRDDMPDGGA EHFAKTVSYL 200
    KERNPKILVE CLTPDFRGDL KAIEKVALSG LDVYAHNVET VPELQRKVRD 250
    PRANFDQSLR VLKHAKEVRP DVISKTSIML GLGENDEQVY ATMKALREAD 300
    VDCLTLGQYM QPTKRHLKVE EYITPEKFKY WEKVGNELGF HYTASGPLVR 350
    SSYKAGEFFL KNLVAKRKTK AL 372
    Length:372
    Mass (Da):42,035
    Last modified:April 12, 2005 - v1
    Checksum:iAA6C54F92133B3A8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT021177 mRNA. Translation: AAX31359.1.
    RefSeqiNP_001017944.1. NM_001017944.1.
    UniGeneiBt.9756.

    Genome annotation databases

    EnsembliENSBTAT00000019299; ENSBTAP00000019299; ENSBTAG00000014520.
    GeneIDi530865.
    KEGGibta:530865.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BT021177 mRNA. Translation: AAX31359.1 .
    RefSeqi NP_001017944.1. NM_001017944.1.
    UniGenei Bt.9756.

    3D structure databases

    ProteinModelPortali Q5BIP7.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q5BIP7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000019299 ; ENSBTAP00000019299 ; ENSBTAG00000014520 .
    GeneIDi 530865.
    KEGGi bta:530865.

    Organism-specific databases

    CTDi 11019.

    Phylogenomic databases

    eggNOGi COG0320.
    GeneTreei ENSGT00390000006234.
    HOGENOMi HOG000235998.
    HOVERGENi HBG023328.
    InParanoidi Q5BIP7.
    KOi K03644.
    OMAi PEEPYNT.
    TreeFami TF300817.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Miscellaneous databases

    NextBioi 20875341.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiLIAS_BOVIN
    AccessioniPrimary (citable) accession number: Q5BIP7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: April 12, 2005
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3