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Protein

DNA excision repair protein ERCC-8

Gene

ERCC8

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair. The CSA complex (DCX(ERCC8) complex) promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair. It is required for the recruitment of XAB2, HMGN1 and TCEA1/TFIIS to a transcription-coupled repair complex which removes RNA polymerase II-blocking lesions from the transcribed strand of active genes (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-BTA-6781823 Formation of TC-NER Pre-Incision Complex
R-BTA-6782135 Dual incision in TC-NER
R-BTA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-BTA-8951664 Neddylation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
DNA excision repair protein ERCC-8
Gene namesi
Name:ERCC8
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 20

Organism-specific databases

VGNCiVGNC:28574 ERCC8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000509691 – 397DNA excision repair protein ERCC-8Add BLAST397

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei391PhosphoserineBy similarity1
Modified residuei392PhosphoserineBy similarity1
Modified residuei393PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5BIM8

Expressioni

Gene expression databases

BgeeiENSBTAG00000021606

Interactioni

Subunit structurei

Part of the CSA complex (DCX(ERCC8) complex), a DCX E3 ubiquitin-protein ligase complex containing ERCC8, RBX1, DDB1 and CUL4A; the CSA complex interacts with RNA polymerase II; upon UV irradiation it interacts with the COP9 signalosome and preferentially with the hyperphosphorylated form of RNA polymerase II. Interacts with ERCC6 and KIAA1530/UVSSA. Interacts with a subunit of RNA polymerase II TFIIH. Interacts with DDB1.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000028792

Structurei

3D structure databases

ProteinModelPortaliQ5BIM8
SMRiQ5BIM8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati41 – 81WD 1Add BLAST41
Repeati97 – 137WD 2Add BLAST41
Repeati184 – 224WD 3Add BLAST41
Repeati243 – 282WD 4Add BLAST40
Repeati332 – 371WD 5Add BLAST40

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG4283 Eukaryota
ENOG410Z0MQ LUCA
GeneTreeiENSGT00390000009065
HOGENOMiHOG000248233
HOVERGENiHBG005409
InParanoidiQ5BIM8
KOiK10570
OMAiTMLRGHY
OrthoDBiEOG091G09HW
TreeFamiTF101237

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00400 WD40, 4 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 5 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 2 hits
PS50082 WD_REPEATS_2, 5 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Q5BIM8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGFLSARQA GLEDPLRLRR AESTRRVLGL ELNKDRDVER IHCSGVNTLD
60 70 80 90 100
IEPVEGRYML SGGSDGVIVL YDLENSSRQP YYTCKAVCSV GRSHPDVHKY
110 120 130 140 150
SVETVQWYPH DTGMFTSSSF DKTLKVWDTN TLQIADVFNF EETVYSHHMS
160 170 180 190 200
PVATKHCLVA VGTRGPKVQL CDLKSGSCSH ILQGHRQEIL AVSWSPRYEY
210 220 230 240 250
ILATASADSR AKLWDVRRAS GCLITLDQHN GKKSQAAESA NTAHNGKVNG
260 270 280 290 300
LCFTSDGLHL LTVGTDNRMR LWNSSNGENT LVNYGKVYND SRKGLKFTVS
310 320 330 340 350
SGCSSEFVFV PYGSTIAVYT IYSGEQITML KGHYKSVDCC VFQSNFQELY
360 370 380 390
SGSRDCNILA WVPSLCESVP DDDDETSTRS QLNPAFEDAW SSSDEEG
Length:397
Mass (Da):44,113
Last modified:April 12, 2005 - v1
Checksum:i2D787B4C80CDA03F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021196 mRNA Translation: AAX31378.1
RefSeqiNP_001103651.1, NM_001110181.1
UniGeneiBt.33395

Genome annotation databases

EnsembliENSBTAT00000028792; ENSBTAP00000028792; ENSBTAG00000021606
GeneIDi518857
KEGGibta:518857

Similar proteinsi

Entry informationi

Entry nameiERCC8_BOVIN
AccessioniPrimary (citable) accession number: Q5BIM8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: April 12, 2005
Last modified: April 25, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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