Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA excision repair protein ERCC-8

Gene

ERCC8

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair. The CSA complex (DCX(ERCC8) complex) promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair. It is required for the recruitment of XAB2, HMGN1 and TCEA1/TFIIS to a transcription-coupled repair complex which removes RNA polymerase II-blocking lesions from the transcribed strand of active genes (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-BTA-6781823. Formation of TC-NER Pre-Incision Complex.
R-BTA-6782135. Dual incision in TC-NER.
R-BTA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA excision repair protein ERCC-8
Gene namesi
Name:ERCC8
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 20

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 397397DNA excision repair protein ERCC-8PRO_0000050969Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei391 – 3911PhosphoserineBy similarity
Modified residuei392 – 3921PhosphoserineBy similarity
Modified residuei393 – 3931PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5BIM8.

Interactioni

Subunit structurei

Part of the CSA complex (DCX(ERCC8) complex), a DCX E3 ubiquitin-protein ligase complex containing ERCC8, RBX1, DDB1 and CUL4A; the CSA complex interacts with RNA polymerase II; upon UV irradiation it interacts with the COP9 signalosome and preferentially with the hyperphosphorylated form of RNA polymerase II. Interacts with ERCC6 and KIAA1530/UVSSA. Interacts with a subunit of RNA polymerase II TFIIH. Interacts with DDB1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000028792.

Structurei

3D structure databases

ProteinModelPortaliQ5BIM8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati41 – 8141WD 1Add
BLAST
Repeati97 – 13741WD 2Add
BLAST
Repeati184 – 22441WD 3Add
BLAST
Repeati243 – 28240WD 4Add
BLAST
Repeati332 – 37140WD 5Add
BLAST

Sequence similaritiesi

Contains 5 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG4283. Eukaryota.
ENOG410Z0MQ. LUCA.
GeneTreeiENSGT00390000009065.
HOGENOMiHOG000248233.
HOVERGENiHBG005409.
InParanoidiQ5BIM8.
KOiK10570.
OMAiFEGTVYS.
OrthoDBiEOG7F511T.
TreeFamiTF101237.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5BIM8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGFLSARQA GLEDPLRLRR AESTRRVLGL ELNKDRDVER IHCSGVNTLD
60 70 80 90 100
IEPVEGRYML SGGSDGVIVL YDLENSSRQP YYTCKAVCSV GRSHPDVHKY
110 120 130 140 150
SVETVQWYPH DTGMFTSSSF DKTLKVWDTN TLQIADVFNF EETVYSHHMS
160 170 180 190 200
PVATKHCLVA VGTRGPKVQL CDLKSGSCSH ILQGHRQEIL AVSWSPRYEY
210 220 230 240 250
ILATASADSR AKLWDVRRAS GCLITLDQHN GKKSQAAESA NTAHNGKVNG
260 270 280 290 300
LCFTSDGLHL LTVGTDNRMR LWNSSNGENT LVNYGKVYND SRKGLKFTVS
310 320 330 340 350
SGCSSEFVFV PYGSTIAVYT IYSGEQITML KGHYKSVDCC VFQSNFQELY
360 370 380 390
SGSRDCNILA WVPSLCESVP DDDDETSTRS QLNPAFEDAW SSSDEEG
Length:397
Mass (Da):44,113
Last modified:April 12, 2005 - v1
Checksum:i2D787B4C80CDA03F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021196 mRNA. Translation: AAX31378.1.
RefSeqiNP_001103651.1. NM_001110181.1.
UniGeneiBt.33395.

Genome annotation databases

EnsembliENSBTAT00000028792; ENSBTAP00000028792; ENSBTAG00000021606.
GeneIDi518857.
KEGGibta:518857.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021196 mRNA. Translation: AAX31378.1.
RefSeqiNP_001103651.1. NM_001110181.1.
UniGeneiBt.33395.

3D structure databases

ProteinModelPortaliQ5BIM8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000028792.

Proteomic databases

PaxDbiQ5BIM8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000028792; ENSBTAP00000028792; ENSBTAG00000021606.
GeneIDi518857.
KEGGibta:518857.

Organism-specific databases

CTDi1161.

Phylogenomic databases

eggNOGiKOG4283. Eukaryota.
ENOG410Z0MQ. LUCA.
GeneTreeiENSGT00390000009065.
HOGENOMiHOG000248233.
HOVERGENiHBG005409.
InParanoidiQ5BIM8.
KOiK10570.
OMAiFEGTVYS.
OrthoDBiEOG7F511T.
TreeFamiTF101237.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-BTA-6781823. Formation of TC-NER Pre-Incision Complex.
R-BTA-6782135. Dual incision in TC-NER.
R-BTA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiERCC8_BOVIN
AccessioniPrimary (citable) accession number: Q5BIM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: April 12, 2005
Last modified: June 8, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.