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Protein

Protrudin

Gene

ZFYVE27

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Key regulator of RAB11-dependent vesicular trafficking during neurite extension through polarized membrane transport. Promotes axonal elongation and contributes to the establishment of neuronal cell polarity. Involved in nerve growth factor-induced neurite formation in VAPA-dependent manner. Contributes to both the formation and stabilization of the tubular ER network. Involved in ER morphogenesis by regulating the sheet-to-tubule balance and possibly the density of tubule interconnections. Acts as an adapter protein that facilitates the interaction of KIF5A with VAPA, VAPB, SURF4, RAB11A, RAB11B and RTN3 and the ZFYVE27-KIF5A complex contributes to the transport of these proteins in neurons. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a KIF5A/B-dependent manner.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri337 – 403FYVE-typePROSITE-ProRule annotationAdd BLAST67

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protrudin
Alternative name(s):
Zinc finger FYVE domain-containing protein 27
Gene namesi
Name:ZFYVE27
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 26

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 66CytoplasmicBy similarityAdd BLAST66
Transmembranei67 – 87HelicalSequence analysisAdd BLAST21
Topological domaini88LumenalBy similarity1
Transmembranei89 – 109HelicalSequence analysisAdd BLAST21
Topological domaini110 – 187CytoplasmicBy similarityAdd BLAST78
Transmembranei188 – 208HelicalSequence analysisAdd BLAST21
Topological domaini209 – 404ExtracellularBy similarityAdd BLAST196

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Endoplasmic reticulum, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002456001 – 404ProtrudinAdd BLAST404

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi209N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Phosphorylated. Phosphorylation is induced by NGF through the MAPK/ERK pathway and modulates interaction with RAB11A (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ5BIM5.
PRIDEiQ5BIM5.

Expressioni

Gene expression databases

BgeeiENSBTAG00000018564.

Interactioni

Subunit structurei

Can form homooligomers (monomers, dimers and tetramers). Interacts with RAB11A (GDP-bound form); regulates RAB11A. Interacts with FKBP8; may negatively regulate ZFYVE27 phosphorylation. Interacts with VAPA (via MSP domain); may regulate ZFYVE27 retention in the endoplasmic reticulum and its function in cell projections formation. Interacts with VAPB (via MSP domain). Interacts with RAB11B (GDP-bound form), REEP1, REEP5, ATL1, ATL2, ATL3, SPAST, SURF4, KIF5A, KIF5B, KIF5C and RTN3.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000024705.

Structurei

3D structure databases

ProteinModelPortaliQ5BIM5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 205Sufficient for localization to endoplasmic reticulum tubular network and for interactions with REEP1, REEP5, ATL1, ATL2, ATL3 and SPASTBy similarityAdd BLAST205
Regioni1 – 92Sufficient for homooligomerizationBy similarityAdd BLAST92
Regioni51 – 64Necessary for interaction with RAB11A and function in neurite outgrowthBy similarityAdd BLAST14
Regioni271 – 354Necessary for interaction with KIF5ABy similarityAdd BLAST84
Regioni286 – 292Necessary for interaction with VAPABy similarity7

Sequence similaritiesi

Contains 1 FYVE-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri337 – 403FYVE-typePROSITE-ProRule annotationAdd BLAST67

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IJBT. Eukaryota.
ENOG4111KCR. LUCA.
GeneTreeiENSGT00390000013298.
HOGENOMiHOG000155785.
HOVERGENiHBG054907.
InParanoidiQ5BIM5.
KOiK19368.
OMAiTVCMLYL.
OrthoDBiEOG091G0QCA.
TreeFamiTF331044.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50178. ZF_FYVE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5BIM5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTSEREGCG PEVSPSTVPE ATLESLPVPT KLPAFDLFNL VLSYKRLEVY
60 70 80 90 100
LEPLKDAGDG VRYLLRWQTP LCSLLTCLGL NVLFLTLNEG AWYSVGALMI
110 120 130 140 150
SVPALLGYLQ EGCQARLSES ELMRRKYHSV RQEDLQRVRL SRPEAVAEVK
160 170 180 190 200
SFLIQLEALL SRLCGTCEAA YRVLHWENPA VSSQFYGALL GTVCMLYLLP
210 220 230 240 250
LCWVLALLNS TLFLGNVEFF RVVSEYRASL QRRMNPKQEE SAFESPPPSD
260 270 280 290 300
AGGKGALVDC TPAPTPTEDL TPGSVEEAEE AEPDEEFKDA IEEDDEGAPC
310 320 330 340 350
PAEDELVLQD NGFLSKNEVL RSKVSRLTER LRKRYPTNNY GSCTGCSATF
360 370 380 390 400
SVLKKRRNCS NCGNIFCSRC CSFKVPRSSM GATAPEAQRE TVFVCASCNQ

TLSK
Length:404
Mass (Da):44,777
Last modified:April 12, 2005 - v1
Checksum:iE03B5E78B1033F88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021199 mRNA. Translation: AAX31381.1.
BC134516 mRNA. Translation: AAI34517.1.
RefSeqiNP_001017939.1. NM_001017939.1.
UniGeneiBt.54620.

Genome annotation databases

EnsembliENSBTAT00000024705; ENSBTAP00000024705; ENSBTAG00000018564.
GeneIDi514383.
KEGGibta:514383.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT021199 mRNA. Translation: AAX31381.1.
BC134516 mRNA. Translation: AAI34517.1.
RefSeqiNP_001017939.1. NM_001017939.1.
UniGeneiBt.54620.

3D structure databases

ProteinModelPortaliQ5BIM5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000024705.

Proteomic databases

PaxDbiQ5BIM5.
PRIDEiQ5BIM5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000024705; ENSBTAP00000024705; ENSBTAG00000018564.
GeneIDi514383.
KEGGibta:514383.

Organism-specific databases

CTDi118813.

Phylogenomic databases

eggNOGiENOG410IJBT. Eukaryota.
ENOG4111KCR. LUCA.
GeneTreeiENSGT00390000013298.
HOGENOMiHOG000155785.
HOVERGENiHBG054907.
InParanoidiQ5BIM5.
KOiK19368.
OMAiTVCMLYL.
OrthoDBiEOG091G0QCA.
TreeFamiTF331044.

Gene expression databases

BgeeiENSBTAG00000018564.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR000306. Znf_FYVE.
IPR017455. Znf_FYVE-rel.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF01363. FYVE. 1 hit.
[Graphical view]
SMARTiSM00064. FYVE. 1 hit.
[Graphical view]
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS50178. ZF_FYVE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZFY27_BOVIN
AccessioniPrimary (citable) accession number: Q5BIM5
Secondary accession number(s): A4IFC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: April 12, 2005
Last modified: November 2, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.