Reviewed, UniProtKB/Swiss-Prot Q5BH83 (DNLI4_EMENI)

Names and origin

Protein names

Recommended name:
    DNA ligase 4
    EC=6.5.1.1
Alternative name(s):
    DNA ligase IV
    Polydeoxyribonucleotide synthase [ATP] 4

Gene names

Name:	lig4
ORF Names:	AN0097

Organism

Emericella nidulans (Aspergillus nidulans) [Complete proteome]

Taxonomic identifier

162425 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella

Protein attributes

Sequence length	1009 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
Cofactor	Magnesium By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family. Contains 2 BRCT domains.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair DNA replication
Cellular component	Nucleus
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1009	1009	DNA ligase 4		PRO_0000278382
Regions
	Domain	715 – 808	94	BRCT 1
	Domain	887 – 995	109	BRCT 2
Sites
	Active site	317	1	N6-AMP-lysine intermediate By similarity
	Metal binding	384	1	Magnesium 1 Potential
	Metal binding	484	1	Magnesium 2 Potential
	Binding site	315	1	ATP By similarity
	Binding site	322	1	ATP By similarity
	Binding site	344	1	ATP By similarity
	Binding site	489	1	ATP By similarity
	Binding site	500	1	ATP By similarity
	Binding site	506	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5BH83-1 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 60055FF8D2E80557	FASTA	1,009	115,689
	10 20 30 40 50 60 METDQDMHDQ AMAGEETDLD EKYPNRPQNK APTLPFHDLY ETLFRPLREI KKKPVGPAGN 70 80 90 100 110 120 RRKAGPHGLS AANLNPIERR RDIIERFISR WRKEVGDDIY PAFRLILPDK DRDRAMYGMK 130 140 150 160 170 180 EKIIGKMLVN IMKIDKNSED GFNLLNWKLP GQSATSSMAG DFAGRCYDVV SKRPMRTEFG 190 200 210 220 230 240 NMLIEEVNEK LDQLSSASKE EEQLPILAEF YRRMNPEELA WLIRIILRQM KLGATERTFF 250 260 270 280 290 300 DVWHPDAENL YSISSSLRRV CWELHDPNIR LDAEDRGVSL MQCFQPQLAQ FQMQSLDRMI 310 320 330 340 350 360 ARMRPTEDDP VFWIEEKLDG ERMQLHMVSD ASAPGGRRFR FWSRKAKDYT YLYGNGIYDE 370 380 390 400 410 420 AGSLTRHLKD AFADGVDNLI LDGEMITWNT EQDAPEPFGT LKTAALSEQR NPFRQGIHPL 430 440 450 460 470 480 FRVFDILYLN GRDLTRYTLR DRRNALQKVI KPVHRRFEVH SYEEATTKAE VEASLRKAVA 490 500 510 520 530 540 EASEGLVLKN PRSPYRLNER HDDWMKVKPE YMTEFGESLD LVVIGGYYGS GHRGGKLSSF 550 560 570 580 590 600 LCGLRVDEGQ SSQGSNPTKC YSFCKVGGGF TAADYANIRH HTDGKWVEWN PKKPPTTYIE 610 620 630 640 650 660 LAGGDSQYER PDMWIKPEDS VVICVKAASV SVSDQFRIGL TLRFPRFKRL RMDKDWKSAL 670 680 690 700 710 720 SVQEFLDLKS HAEQEHREKE FNVENFRKKR VKKTTKKPLA IAGYDENAEV QYAGPSGHIF 730 740 750 760 770 780 DGLNFFILTD SNAPVKKSKA ELENLVKANG GRIFQTNDAV PDTICIADRR TVKAASLQKK 790 800 810 820 830 840 GDIDIIRPSW ILDCIKQSEI DAGLPDLLLP LEPGHMFFMT KDKEEIVAGS LDQFNDSYAR 850 860 870 880 890 900 DITVEELRNL LDQMAKDGKT DSFCSPEAIQ KVTEHIQEKV DSGWTMPCGW LFKGLVLCFP 910 920 930 940 950 960 ENENDSASEG PEPKQSQRIH LAQNTAKFAG ASVTTSLKDT SITHVVVDPD FTSSELPKLR 970 980 990 1000 RTLSTRRKLP HIVKVNWIED SWKENTLLDE EQHMPVYMRR YPNIQLMKV

References

[1]

"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed: 16372000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC A4 / M139.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACD01000003 Genomic DNA. Translation: EAA65275.1.
RefSeq	XP_657701.1.
3D structure databases
SMR	Q5BH83. Positions 718-992.
ModBase	Search...
Genome annotation databases
GeneID	2875873.
KEGG	ani:AN0097.2.
Phylogenomic databases
PhylomeDB	Q5BH83.
Enzyme and pathway databases
BRENDA	6.5.1.1. 3859.
Family and domain databases
InterPro	IPR001357. BRCT. IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF00533. BRCT. 1 hit. PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. [Graphical view]
SMART	SM00292. BRCT. 2 hits. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS50172. BRCT. 2 hits. PS00697. DNA_LIGASE_A1. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI4_EMENI

Accession

Primary (citable) accession number: Q5BH83

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	April 26, 2005
Last modified:	February 9, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)