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Q5BH83

- DNLI4_EMENI

UniProt

Q5BH83 - DNLI4_EMENI

Protein

DNA ligase 4

Gene

lig4

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (26 Apr 2005)
      Previous versions | rss
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    Functioni

    Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining By similarity.By similarity

    Catalytic activityi

    ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).PROSITE-ProRule annotation

    Cofactori

    Magnesium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei315 – 3151ATPBy similarity
    Active sitei317 – 3171N6-AMP-lysine intermediatePROSITE-ProRule annotation
    Binding sitei322 – 3221ATPBy similarity
    Binding sitei344 – 3441ATPBy similarity
    Metal bindingi384 – 3841Magnesium 1Sequence Analysis
    Metal bindingi484 – 4841Magnesium 2Sequence Analysis
    Binding sitei489 – 4891ATPBy similarity
    Binding sitei500 – 5001ATPBy similarity
    Binding sitei506 – 5061ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: InterPro
    3. DNA ligase (ATP) activity Source: RefGenome
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA ligation Source: RefGenome
    2. DNA ligation involved in DNA repair Source: InterPro
    3. DNA recombination Source: UniProtKB-KW
    4. double-strand break repair via nonhomologous end joining Source: RefGenome
    5. lagging strand elongation Source: RefGenome
    6. nucleotide-excision repair Source: RefGenome

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair, DNA replication

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA ligase 4 (EC:6.5.1.1)
    Alternative name(s):
    DNA ligase IV
    Polydeoxyribonucleotide synthase [ATP] 4
    Gene namesi
    Name:lig4
    ORF Names:AN0097
    OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
    Taxonomic identifieri227321 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000000560: Chromosome VIII

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. chromosome Source: RefGenome
    2. DNA ligase IV complex Source: RefGenome

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10091009DNA ligase 4PRO_0000278382Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi162425.CADANIAP00002653.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5BH83.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini715 – 80894BRCT 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini887 – 995109BRCT 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ATP-dependent DNA ligase family.Curated
    Contains 2 BRCT domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1793.
    HOGENOMiHOG000176213.
    KOiK10777.
    OMAiHMCPSTK.
    OrthoDBiEOG7QC84G.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.40.50.10190. 2 hits.
    InterProiIPR001357. BRCT_dom.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view]
    PfamiPF00533. BRCT. 1 hit.
    PF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view]
    SMARTiSM00292. BRCT. 2 hits.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF52113. SSF52113. 2 hits.
    TIGRFAMsiTIGR00574. dnl1. 1 hit.
    PROSITEiPS50172. BRCT. 2 hits.
    PS00697. DNA_LIGASE_A1. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5BH83-1 [UniParc]FASTAAdd to Basket

    « Hide

    METDQDMHDQ AMAGEETDLD EKYPNRPQNK APTLPFHDLY ETLFRPLREI     50
    KKKPVGPAGN RRKAGPHGLS AANLNPIERR RDIIERFISR WRKEVGDDIY 100
    PAFRLILPDK DRDRAMYGMK EKIIGKMLVN IMKIDKNSED GFNLLNWKLP 150
    GQSATSSMAG DFAGRCYDVV SKRPMRTEFG NMLIEEVNEK LDQLSSASKE 200
    EEQLPILAEF YRRMNPEELA WLIRIILRQM KLGATERTFF DVWHPDAENL 250
    YSISSSLRRV CWELHDPNIR LDAEDRGVSL MQCFQPQLAQ FQMQSLDRMI 300
    ARMRPTEDDP VFWIEEKLDG ERMQLHMVSD ASAPGGRRFR FWSRKAKDYT 350
    YLYGNGIYDE AGSLTRHLKD AFADGVDNLI LDGEMITWNT EQDAPEPFGT 400
    LKTAALSEQR NPFRQGIHPL FRVFDILYLN GRDLTRYTLR DRRNALQKVI 450
    KPVHRRFEVH SYEEATTKAE VEASLRKAVA EASEGLVLKN PRSPYRLNER 500
    HDDWMKVKPE YMTEFGESLD LVVIGGYYGS GHRGGKLSSF LCGLRVDEGQ 550
    SSQGSNPTKC YSFCKVGGGF TAADYANIRH HTDGKWVEWN PKKPPTTYIE 600
    LAGGDSQYER PDMWIKPEDS VVICVKAASV SVSDQFRIGL TLRFPRFKRL 650
    RMDKDWKSAL SVQEFLDLKS HAEQEHREKE FNVENFRKKR VKKTTKKPLA 700
    IAGYDENAEV QYAGPSGHIF DGLNFFILTD SNAPVKKSKA ELENLVKANG 750
    GRIFQTNDAV PDTICIADRR TVKAASLQKK GDIDIIRPSW ILDCIKQSEI 800
    DAGLPDLLLP LEPGHMFFMT KDKEEIVAGS LDQFNDSYAR DITVEELRNL 850
    LDQMAKDGKT DSFCSPEAIQ KVTEHIQEKV DSGWTMPCGW LFKGLVLCFP 900
    ENENDSASEG PEPKQSQRIH LAQNTAKFAG ASVTTSLKDT SITHVVVDPD 950
    FTSSELPKLR RTLSTRRKLP HIVKVNWIED SWKENTLLDE EQHMPVYMRR 1000
    YPNIQLMKV 1009
    Length:1,009
    Mass (Da):115,689
    Last modified:April 26, 2005 - v1
    Checksum:i60055FF8D2E80557
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACD01000003 Genomic DNA. Translation: EAA65275.1.
    BN001308 Genomic DNA. Translation: CBF90206.1.
    RefSeqiXP_657701.1. XM_652609.1.

    Genome annotation databases

    EnsemblFungiiCADANIAT00002653; CADANIAP00002653; CADANIAG00002653.
    GeneIDi2875873.
    KEGGiani:AN0097.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACD01000003 Genomic DNA. Translation: EAA65275.1 .
    BN001308 Genomic DNA. Translation: CBF90206.1 .
    RefSeqi XP_657701.1. XM_652609.1.

    3D structure databases

    ProteinModelPortali Q5BH83.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 162425.CADANIAP00002653.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADANIAT00002653 ; CADANIAP00002653 ; CADANIAG00002653 .
    GeneIDi 2875873.
    KEGGi ani:AN0097.2.

    Phylogenomic databases

    eggNOGi COG1793.
    HOGENOMi HOG000176213.
    KOi K10777.
    OMAi HMCPSTK.
    OrthoDBi EOG7QC84G.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.40.50.10190. 2 hits.
    InterProi IPR001357. BRCT_dom.
    IPR000977. DNA_ligase_ATP-dep.
    IPR012309. DNA_ligase_ATP-dep_C.
    IPR012310. DNA_ligase_ATP-dep_cent.
    IPR016059. DNA_ligase_ATP-dep_CS.
    IPR012308. DNA_ligase_ATP-dep_N.
    IPR012340. NA-bd_OB-fold.
    [Graphical view ]
    Pfami PF00533. BRCT. 1 hit.
    PF04679. DNA_ligase_A_C. 1 hit.
    PF01068. DNA_ligase_A_M. 1 hit.
    PF04675. DNA_ligase_A_N. 1 hit.
    [Graphical view ]
    SMARTi SM00292. BRCT. 2 hits.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF52113. SSF52113. 2 hits.
    TIGRFAMsi TIGR00574. dnl1. 1 hit.
    PROSITEi PS50172. BRCT. 2 hits.
    PS00697. DNA_LIGASE_A1. 1 hit.
    PS50160. DNA_LIGASE_A3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
    2. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
      Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
      , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
      Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

    Entry informationi

    Entry nameiDNLI4_EMENI
    AccessioniPrimary (citable) accession number: Q5BH83
    Secondary accession number(s): C8VQN0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 20, 2007
    Last sequence update: April 26, 2005
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3