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Q5BH83 (DNLI4_EMENI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA ligase 4

EC=6.5.1.1
Alternative name(s):
DNA ligase IV
Polydeoxyribonucleotide synthase [ATP] 4
Gene names
Name:lig4
ORF Names:AN0097
OrganismEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) [Reference proteome]
Taxonomic identifier227321 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length1009 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining By similarity.

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).

Cofactor

Magnesium By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the ATP-dependent DNA ligase family.

Contains 2 BRCT domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10091009DNA ligase 4
PRO_0000278382

Regions

Domain715 – 80894BRCT 1
Domain887 – 995109BRCT 2

Sites

Active site3171N6-AMP-lysine intermediate By similarity
Metal binding3841Magnesium 1 Potential
Metal binding4841Magnesium 2 Potential
Binding site3151ATP By similarity
Binding site3221ATP By similarity
Binding site3441ATP By similarity
Binding site4891ATP By similarity
Binding site5001ATP By similarity
Binding site5061ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5BH83 [UniParc].

Last modified April 26, 2005. Version 1.
Checksum: 60055FF8D2E80557

FASTA1,009115,689
        10         20         30         40         50         60 
METDQDMHDQ AMAGEETDLD EKYPNRPQNK APTLPFHDLY ETLFRPLREI KKKPVGPAGN 

        70         80         90        100        110        120 
RRKAGPHGLS AANLNPIERR RDIIERFISR WRKEVGDDIY PAFRLILPDK DRDRAMYGMK 

       130        140        150        160        170        180 
EKIIGKMLVN IMKIDKNSED GFNLLNWKLP GQSATSSMAG DFAGRCYDVV SKRPMRTEFG 

       190        200        210        220        230        240 
NMLIEEVNEK LDQLSSASKE EEQLPILAEF YRRMNPEELA WLIRIILRQM KLGATERTFF 

       250        260        270        280        290        300 
DVWHPDAENL YSISSSLRRV CWELHDPNIR LDAEDRGVSL MQCFQPQLAQ FQMQSLDRMI 

       310        320        330        340        350        360 
ARMRPTEDDP VFWIEEKLDG ERMQLHMVSD ASAPGGRRFR FWSRKAKDYT YLYGNGIYDE 

       370        380        390        400        410        420 
AGSLTRHLKD AFADGVDNLI LDGEMITWNT EQDAPEPFGT LKTAALSEQR NPFRQGIHPL 

       430        440        450        460        470        480 
FRVFDILYLN GRDLTRYTLR DRRNALQKVI KPVHRRFEVH SYEEATTKAE VEASLRKAVA 

       490        500        510        520        530        540 
EASEGLVLKN PRSPYRLNER HDDWMKVKPE YMTEFGESLD LVVIGGYYGS GHRGGKLSSF 

       550        560        570        580        590        600 
LCGLRVDEGQ SSQGSNPTKC YSFCKVGGGF TAADYANIRH HTDGKWVEWN PKKPPTTYIE 

       610        620        630        640        650        660 
LAGGDSQYER PDMWIKPEDS VVICVKAASV SVSDQFRIGL TLRFPRFKRL RMDKDWKSAL 

       670        680        690        700        710        720 
SVQEFLDLKS HAEQEHREKE FNVENFRKKR VKKTTKKPLA IAGYDENAEV QYAGPSGHIF 

       730        740        750        760        770        780 
DGLNFFILTD SNAPVKKSKA ELENLVKANG GRIFQTNDAV PDTICIADRR TVKAASLQKK 

       790        800        810        820        830        840 
GDIDIIRPSW ILDCIKQSEI DAGLPDLLLP LEPGHMFFMT KDKEEIVAGS LDQFNDSYAR 

       850        860        870        880        890        900 
DITVEELRNL LDQMAKDGKT DSFCSPEAIQ KVTEHIQEKV DSGWTMPCGW LFKGLVLCFP 

       910        920        930        940        950        960 
ENENDSASEG PEPKQSQRIH LAQNTAKFAG ASVTTSLKDT SITHVVVDPD FTSSELPKLR 

       970        980        990       1000 
RTLSTRRKLP HIVKVNWIED SWKENTLLDE EQHMPVYMRR YPNIQLMKV 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae."
Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. expand/collapse author list , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.
Nature 438:1105-1115(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[2]"The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G. expand/collapse author list , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AACD01000003 Genomic DNA. Translation: EAA65275.1.
BN001308 Genomic DNA. Translation: CBF90206.1.
RefSeqXP_657701.1. XM_652609.1.

3D structure databases

ProteinModelPortalQ5BH83.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING162425.CADANIAP00002653.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADANIAT00002653; CADANIAP00002653; CADANIAG00002653.
GeneID2875873.
KEGGani:AN0097.2.

Phylogenomic databases

eggNOGCOG1793.
HOGENOMHOG000176213.
KOK10777.
OMAHMCPSTK.
OrthoDBEOG7QC84G.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
3.40.50.10190. 2 hits.
InterProIPR001357. BRCT_dom.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF00533. BRCT. 1 hit.
PF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 1 hit.
[Graphical view]
SMARTSM00292. BRCT. 2 hits.
[Graphical view]
SUPFAMSSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 2 hits.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS50172. BRCT. 2 hits.
PS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDNLI4_EMENI
AccessionPrimary (citable) accession number: Q5BH83
Secondary accession number(s): C8VQN0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: April 26, 2005
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families