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Protein

tRNA wybutosine-synthesizing protein 4

Gene

ppm2

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Probable S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. May methylate the carboxyl group of leucine residues to form alpha-leucine ester residues (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + 7-((3S)-3-amino-3-carboxypropyl)wyosine(37) in tRNA(Phe) = S-adenosyl-L-homocysteine + 7-((3S)-3-amino-3-(methoxycarbonyl)propyl)wyosine(37) in tRNA(Phe).
S-adenosyl-L-methionine + 7-((3S)-3-amino-3-(methoxycarbonyl)propyl)wyosine(37) in tRNA(Phe) + CO2 = S-adenosyl-L-homocysteine + wybutosine(37) in tRNA(Phe).

Pathwayi: wybutosine-tRNA(Phe) biosynthesis

This protein is involved in the pathway wybutosine-tRNA(Phe) biosynthesis, which is part of tRNA modification.
View all proteins of this organism that are known to be involved in the pathway wybutosine-tRNA(Phe) biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei81 – 811S-adenosyl-L-methionineBy similarity
Binding sitei107 – 1071S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Binding sitei134 – 1341S-adenosyl-L-methionineBy similarity
Binding sitei208 – 2081S-adenosyl-L-methionine; via carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00375.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA wybutosine-synthesizing protein 4 (EC:2.1.1.290, EC:2.3.1.231)
Short name:
tRNA-yW synthesizing protein 4
Alternative name(s):
Leucine carboxyl methyltransferase 2
tRNA(Phe) (7-(3-amino-3-(methoxycarbonyl)propyl)wyosine(37)-N)-methoxycarbonyltransferase
tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase
Gene namesi
Name:ppm2
Synonyms:tyw4
ORF Names:AN0128
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome VIII
  • UP000005890 Componenti: Partially assembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:AN0128.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10681068tRNA wybutosine-synthesizing protein 4PRO_0000226141Add
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini876 – 1024149JmjCPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni181 – 1822S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Contains 1 JmjC domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000175968.
InParanoidiQ5BH52.
KOiK15451.
OMAiLSCWRHS.
OrthoDBiEOG092C0IJS.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR003347. JmjC_dom.
IPR015915. Kelch-typ_b-propeller.
IPR007213. Ppm1/Ppm2/Tcmp.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13600. PTHR13600. 1 hit.
PfamiPF04072. LCM. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51184. JMJC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5BH52-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCPPEQPAKA MAPSKSNQAA KSAVPTKEEK SADLVMATNN SSIVSKRSVE
60 70 80 90 100
MLYYPEPHFF CHFVKKPQRR APLINRGYWL RMHAMEESVR RFMRESPDKP
110 120 130 140 150
KFVLNLGCGF DPLPFILLSA DRSLCSQTTF VDIDYEKLML NKKAALREAG
160 170 180 190 200
ALTQILEDVE FGPDESAVQI RSGQYVAVGC DLKNLDKLDR VLRAEVLPAE
210 220 230 240 250
CAVLFLAEVS LTYMDVKSAN AVVSWASRLS NDAQFCILEQ YFPDGPSHPF
260 270 280 290 300
AATMMEHFGK LGAPLHSIHE FPSLSDQERR FTEAGWTHAH ARSLWDLWSD
310 320 330 340 350
DEFVPAVLRT SLDSIESFDE WEEFALFASH YFLLHASTRP GTKGSKSAAA
360 370 380 390 400
TADVGPSRQA VTRSNEFRLI PNTSLPTGQR RFGALVPGGE AVIGIHSGWG
410 420 430 440 450
RQTRVADTDV YKTSKDNIDS HARFPSSNDI SARLCHTITA FSDDGDCLLV
460 470 480 490 500
GGRTSPASAL QDTWVRKNNV WQAGSSLPLA RFRHCATRVT LGSDRSSGSV
510 520 530 540 550
LIYGGKTSDG TTLDTWLLWN DNGEGWSSVT VRTMNDAPAP KARFGACLAS
560 570 580 590 600
IDSTNGLLFG GIGPDGTILE DFWTWKLYEE ADGSLCMELT DQTGSLRNIA
610 620 630 640 650
LGFDILPRFG ATVSSTAQGL VVSGGIIPRR VVPFDGEILL LDSATLLDCI
660 670 680 690 700
KNGLPLTTPI LSTIGLDAGF TGPRPLLVGH VSHAISTDQV LLLGGGAVCF
710 720 730 740 750
SFGTFWTRGT WMLCPVGERA VNDWALVCEN VEKPTKAKAA AQIPAKTKKQ
760 770 780 790 800
KASKKYKPEK YKSTTKVTPI RRVTVESADE FQQILEDAQP VIIESADIGP
810 820 830 840 850
CTELWTKEYL VNTVGSDRKV IVHAAETETM SFRTKNFKYE SKTFGTFMDE
860 870 880 890 900
VHAGGRQYLR SISEKQPAKL PANLAADFPS LSSDFRLPEA LKTVVENAHS
910 920 930 940 950
SPLRISGPVT LWLHYDVMAN VLCQIQGEKR LILYPPSDVP HLDVPAGASS
960 970 980 990 1000
SNINVFQNRA DGAIALIPHT SPHEARLKRG DILFIPPLWL HTAAPTGKVS
1010 1020 1030 1040 1050
VAVNVFFRNL SQGYALGRDV YGNRDVQAYE KGRKDLEKLV KSFSGLPPDM
1060
ARFYLLRLAD ELQETAEQ
Length:1,068
Mass (Da):117,417
Last modified:October 19, 2011 - v3
Checksum:i9107053B261AABB2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACD01000004 Genomic DNA. Translation: EAA65306.1.
BN001308 Genomic DNA. Translation: CBF90143.1.
RefSeqiXP_657732.1. XM_652640.1.

Genome annotation databases

EnsemblFungiiCADANIAT00002618; CADANIAP00002618; CADANIAG00002618.
EAA65306; EAA65306; AN0128.2.
GeneIDi2875904.
KEGGiani:AN0128.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACD01000004 Genomic DNA. Translation: EAA65306.1.
BN001308 Genomic DNA. Translation: CBF90143.1.
RefSeqiXP_657732.1. XM_652640.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANIAT00002618; CADANIAP00002618; CADANIAG00002618.
EAA65306; EAA65306; AN0128.2.
GeneIDi2875904.
KEGGiani:AN0128.2.

Organism-specific databases

EuPathDBiFungiDB:AN0128.

Phylogenomic databases

HOGENOMiHOG000175968.
InParanoidiQ5BH52.
KOiK15451.
OMAiLSCWRHS.
OrthoDBiEOG092C0IJS.

Enzyme and pathway databases

UniPathwayiUPA00375.

Family and domain databases

Gene3Di2.120.10.80. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR003347. JmjC_dom.
IPR015915. Kelch-typ_b-propeller.
IPR007213. Ppm1/Ppm2/Tcmp.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR13600. PTHR13600. 1 hit.
PfamiPF04072. LCM. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51184. JMJC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYW4_EMENI
AccessioniPrimary (citable) accession number: Q5BH52
Secondary accession number(s): C8VQJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: October 19, 2011
Last modified: September 7, 2016
This is version 71 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.