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Q5BFT5

- NCPR_EMENI

UniProt

Q5BFT5 - NCPR_EMENI

Protein

NADPH--cytochrome P450 reductase

Gene

cprA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (26 Apr 2005)
      Previous versions | rss
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    Functioni

    This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis By similarity.By similarity

    Catalytic activityi

    NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.By similarity

    Cofactori

    FAD.By similarity
    FMN.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi72 – 765FMNBy similarityPROSITE-ProRule annotation
    Nucleotide bindingi166 – 19732FMNBy similarityPROSITE-ProRule annotationAdd
    BLAST
    Nucleotide bindingi317 – 32812FADBy similarityAdd
    BLAST
    Nucleotide bindingi447 – 45812FADBy similarityAdd
    BLAST
    Nucleotide bindingi547 – 56519NADPBy similarityAdd
    BLAST
    Nucleotide bindingi642 – 65817NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. FMN binding Source: InterPro
    2. iron ion binding Source: InterPro
    3. NADPH-hemoprotein reductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. sterol biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Keywords - Ligandi

    FAD, Flavoprotein, FMN, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADPH--cytochrome P450 reductaseBy similarity (EC:1.6.2.4)
    Short name:
    CPRBy similarity
    Short name:
    P450RBy similarity
    Gene namesi
    Name:cprABy similarity
    ORF Names:AN0595
    OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
    Taxonomic identifieri227321 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000000560: Chromosome VIII

    Subcellular locationi

    Endoplasmic reticulum membrane By similarity; Single-pass membrane protein By similarity. Cell membrane By similarity; Single-pass membrane protein By similarity. Microsome By similarity

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 695695NADPH--cytochrome P450 reductasePRO_0000404332Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi162425.CADANIAP00002079.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5BFT5.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei9 – 3123HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini66 – 221156Flavodoxin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini277 – 538262FAD-binding FR-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.Sequence Analysis
    Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
    Contains 1 flavodoxin-like domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0369.
    HOGENOMiHOG000282027.
    KOiK00327.
    OMAiYQLEWQD.
    OrthoDBiEOG744TKC.

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    InterProiIPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR023208. P450R.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000208. P450R. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5BFT5-1 [UniParc]FASTAAdd to Basket

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    MAQLDTLDVV VLAVLLAGSI AYFTKGTFWA VAKDPYASSG PAMNGVAKAG    50
    KSRNIIEKMD ETGKNCVIFY GSQTGTAEDY ASRLAKEGSQ RFGLKTMVAD 100
    IEEYDYENLD QFPEDKVAFF VLATYGEGEP TDNAVEFYQF ITGDDVSFEG 150
    GGSAEDKPLS SLKYVAFGLG NNTYEHYNAM VRQVDAALTK LGAQRIGSAG 200
    EGDDGAGTME EDFLAWKEPM WAALSEAMNL QEREASYEPV FCVTEDESLT 250
    PEDNSVYLGE PTKGHLEGQP NGPYSAHNPY IAPIVESREL FTVKDRNCLH 300
    MEISIAGTNL TYQTGDHIAI WPTNAGAEVD RFLNVFGLEE KRHSVINIKG 350
    IDVTAKVPIP TPTTYDAAVR YYMEVCAPVS RQFVSTLAAF APDEETKTEI 400
    VRLGSDKDYF HEKITNQCFN IAQALQSITS KPFSNVPFSL LIEGLNKIQP 450
    RYYSISSSSL VQKDKISITA VVESTRLPGA THIVKGVTTN YLLALKQKQN 500
    GDPSPDPHGQ TYAINGPRNK YDGIHVPVHV RHSNFKLPSD PSRPIIMIGP 550
    GTGVAPFRGF IQERAALAAR GEKVGPTVLF FGCRKRDEDF LYKDEWKVFQ 600
    DQLGDSLKII TAFSRESEKK VYVQHRLKEH AELVSDLLKQ KATFYVCGDA 650
    ANMAREVNLV LGQIIAAQRG LPAEKGEEMV KHMRSSGSYQ EDVWS 695
    Length:695
    Mass (Da):76,698
    Last modified:April 26, 2005 - v1
    Checksum:iD3C5316080312354
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACD01000007 Genomic DNA. Translation: EAA66694.1.
    BN001308 Genomic DNA. Translation: CBF89160.1.
    RefSeqiXP_658199.1. XM_653107.1.

    Genome annotation databases

    EnsemblFungiiCADANIAT00002079; CADANIAP00002079; CADANIAG00002079.
    GeneIDi2876366.
    KEGGiani:AN0595.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACD01000007 Genomic DNA. Translation: EAA66694.1 .
    BN001308 Genomic DNA. Translation: CBF89160.1 .
    RefSeqi XP_658199.1. XM_653107.1.

    3D structure databases

    ProteinModelPortali Q5BFT5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 162425.CADANIAP00002079.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADANIAT00002079 ; CADANIAP00002079 ; CADANIAG00002079 .
    GeneIDi 2876366.
    KEGGi ani:AN0595.2.

    Phylogenomic databases

    eggNOGi COG0369.
    HOGENOMi HOG000282027.
    KOi K00327.
    OMAi YQLEWQD.
    OrthoDBi EOG744TKC.

    Family and domain databases

    Gene3Di 1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    InterProi IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR023208. P450R.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000208. P450R. 1 hit.
    PRINTSi PR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMi SSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    PROSITEi PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
    2. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
      Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
      , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
      Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

    Entry informationi

    Entry nameiNCPR_EMENI
    AccessioniPrimary (citable) accession number: Q5BFT5
    Secondary accession number(s): C8VSB1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 8, 2011
    Last sequence update: April 26, 2005
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3