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Protein

Probable beta-galactosidase A

Gene

lacA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei96 – 961SubstrateBy similarity
Binding sitei140 – 1401SubstrateBy similarity
Binding sitei141 – 1411Substrate; via amide nitrogenBy similarity
Binding sitei142 – 1421SubstrateBy similarity
Binding sitei199 – 1991SubstrateBy similarity
Active sitei200 – 2001Proton donorSequence Analysis
Binding sitei260 – 2601SubstrateBy similarity
Active sitei298 – 2981NucleophileSequence Analysis
Binding sitei364 – 3641SubstrateBy similarity

GO - Molecular functioni

  • beta-galactosidase activity Source: ASPGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase A (EC:3.2.1.23)
Alternative name(s):
Lactase A
Gene namesi
Name:lacA
ORF Names:AN0756
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000000560 Componenti: Chromosome VIII

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 1007989Probable beta-galactosidase APRO_0000395220Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi156 – 1561N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi205 ↔ 206By similarity
Disulfide bondi266 ↔ 315By similarity
Glycosylationi405 – 4051N-linked (GlcNAc...)Sequence Analysis
Glycosylationi422 – 4221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi621 – 6211N-linked (GlcNAc...)Sequence Analysis
Glycosylationi740 – 7401N-linked (GlcNAc...)Sequence Analysis
Glycosylationi775 – 7751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi914 – 9141N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ5BFC4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000181922.
InParanoidiQ5BFC4.
OrthoDBiEOG7ZGXBD.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5BFC4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRLLPVWTAA LLAAQAAGVA LTHKLNGFTI TEHPDAEKRE LLQKYVTWDD
60 70 80 90 100
KSLFINGERI MIFGAEIHPW RLPVPSLWRD ILQKVKALGF NCVSFYVDWA
110 120 130 140 150
LLEGKPGEYR AEGSFAWEPF FDAASDLGIY LIARPGPYIN AEASGGGFPG
160 170 180 190 200
WLQRLNGTIR SSDQSYLDAT ENYVSHIGGL IAKYQITNGG PVILYQPDNE
210 220 230 240 250
YSGGCCGQEF PNPDYFQYVI DQARRAGIVV PTISNDAWPG GHNAPGTGKG
260 270 280 290 300
EVDIYGHDNY PLGFDCANPD VWPEGNLPTD YRDLHLEISP STPYALVEYQ
310 320 330 340 350
VGAFDPWGGP GFEQCAALTG YEFERVFHKN TFSFGVGILS LYMTFGGTNW
360 370 380 390 400
GNLGHPGGYT SYDYGSPIKE TREITREKYS ELKLLGNFIK SSPGYLLATP
410 420 430 440 450
GKLTNTTYTN TADLTVTPLL GNGTGSFFVL RHSDYSSQAS TPYKLRLPTS
460 470 480 490 500
AGQLTIPQLG GSLVLNGRDS KVHLVDYDVA GTKILYSTAE VFTWKKFHDG
510 520 530 540 550
KVLVLYGGPG EHHELAVSSK AKVKVVEGLG SGISSKQIRG AVVVAWDVEP
560 570 580 590 600
ARRIVQIGDL KIFLLDRNSA YNYWVPQLGT ETSIPYATEK AVAASVIVKA
610 620 630 640 650
GYLVRTAYVK GRDLHLTADF NATTPVEVIG APKTAENLFI NGKKAHHTVD
660 670 680 690 700
KNGIWSTEVG YSPPKIVLPV LEDLKWKSID TLPEIQPSYD DSPWPDANLP
710 720 730 740 750
TKNTIYPLRT PTSLYASDYG FHTGYLLFRG HFTANGRESN FSIQTQGGQA
760 770 780 790 800
FGSSVWLSGT YLGSWTGDND YQDYNATYTL PSLKAGKEYV FTVVVDNMGL
810 820 830 840 850
NENWIVGQDE MKKPRGILNY ELSGHEASDI TWKLTGNFGG EDYVDKVRGP
860 870 880 890 900
LNEGGLYAER HGYHQPYPPT KSKDWKSSTP LTGLSKPGIS FYTASFDLDI
910 920 930 940 950
KSGWDVPIYF EFGNSTTPAP AYRVQLYVNG WQYGKYVNNI GPQTRFPVPE
960 970 980 990 1000
GILNYKGTNW VAVTLWALEG SGAKLDSFKL VHGIPVRTAL DVEGVELPRY

QSRKGVY
Length:1,007
Mass (Da):111,099
Last modified:July 13, 2010 - v2
Checksum:i0C80D0D7E3893C4F
GO

Sequence cautioni

The sequence CBF88847.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence EAA65398.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACD01000012 Genomic DNA. Translation: EAA65398.1. Sequence problems.
BN001308 Genomic DNA. Translation: CBF88847.1. Sequence problems.
RefSeqiXP_658360.1. XM_653268.1.

Genome annotation databases

EnsemblFungiiCADANIAT00001909; CADANIAP00001909; CADANIAG00001909.
GeneIDi2876531.
KEGGiani:AN0756.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACD01000012 Genomic DNA. Translation: EAA65398.1. Sequence problems.
BN001308 Genomic DNA. Translation: CBF88847.1. Sequence problems.
RefSeqiXP_658360.1. XM_653268.1.

3D structure databases

ProteinModelPortaliQ5BFC4.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANIAT00001909; CADANIAP00001909; CADANIAG00001909.
GeneIDi2876531.
KEGGiani:AN0756.2.

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000181922.
InParanoidiQ5BFC4.
OrthoDBiEOG7ZGXBD.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  2. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
    Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
    , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
    Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Entry informationi

Entry nameiBGALA_EMENI
AccessioniPrimary (citable) accession number: Q5BFC4
Secondary accession number(s): C8VQX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: July 13, 2010
Last modified: July 22, 2015
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.