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Q5BFC4

- BGALA_EMENI

UniProt

Q5BFC4 - BGALA_EMENI

Protein

Probable beta-galactosidase A

Gene

lacA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 2 (13 Jul 2010)
      Previous versions | rss
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    Functioni

    Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei96 – 961SubstrateBy similarity
    Binding sitei140 – 1401SubstrateBy similarity
    Binding sitei141 – 1411Substrate; via amide nitrogenBy similarity
    Binding sitei142 – 1421SubstrateBy similarity
    Binding sitei199 – 1991SubstrateBy similarity
    Active sitei200 – 2001Proton donorSequence Analysis
    Binding sitei260 – 2601SubstrateBy similarity
    Active sitei298 – 2981NucleophileSequence Analysis
    Binding sitei364 – 3641SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: ASPGD

    GO - Biological processi

    1. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation

    Protein family/group databases

    CAZyiGH35. Glycoside Hydrolase Family 35.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable beta-galactosidase A (EC:3.2.1.23)
    Alternative name(s):
    Lactase A
    Gene namesi
    Name:lacA
    ORF Names:AN0756
    OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
    Taxonomic identifieri227321 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000000560: Chromosome VIII

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Chaini19 – 1007989Probable beta-galactosidase APRO_0000395220Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi156 – 1561N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi205 ↔ 206By similarity
    Disulfide bondi266 ↔ 315By similarity
    Glycosylationi405 – 4051N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi422 – 4221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi621 – 6211N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi740 – 7401N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi775 – 7751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi914 – 9141N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi162425.CADANIAP00001909.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5BFC4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 35 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1874.
    HOGENOMiHOG000181922.
    OrthoDBiEOG7ZGXBD.

    Family and domain databases

    Gene3Di2.102.20.10. 1 hit.
    2.60.120.260. 2 hits.
    2.60.390.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR018954. Betagal_dom2.
    IPR025972. BetaGal_dom3.
    IPR025300. BetaGal_jelly_roll_dom.
    IPR008979. Galactose-bd-like.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR23421. PTHR23421. 1 hit.
    PfamiPF10435. BetaGal_dom2. 1 hit.
    PF13363. BetaGal_dom3. 1 hit.
    PF13364. BetaGal_dom4_5. 2 hits.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view]
    PRINTSiPR00742. GLHYDRLASE35.
    SMARTiSM01029. BetaGal_dom2. 1 hit.
    [Graphical view]
    SUPFAMiSSF117100. SSF117100. 1 hit.
    SSF49785. SSF49785. 2 hits.
    SSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5BFC4-1 [UniParc]FASTAAdd to Basket

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    MRLLPVWTAA LLAAQAAGVA LTHKLNGFTI TEHPDAEKRE LLQKYVTWDD     50
    KSLFINGERI MIFGAEIHPW RLPVPSLWRD ILQKVKALGF NCVSFYVDWA 100
    LLEGKPGEYR AEGSFAWEPF FDAASDLGIY LIARPGPYIN AEASGGGFPG 150
    WLQRLNGTIR SSDQSYLDAT ENYVSHIGGL IAKYQITNGG PVILYQPDNE 200
    YSGGCCGQEF PNPDYFQYVI DQARRAGIVV PTISNDAWPG GHNAPGTGKG 250
    EVDIYGHDNY PLGFDCANPD VWPEGNLPTD YRDLHLEISP STPYALVEYQ 300
    VGAFDPWGGP GFEQCAALTG YEFERVFHKN TFSFGVGILS LYMTFGGTNW 350
    GNLGHPGGYT SYDYGSPIKE TREITREKYS ELKLLGNFIK SSPGYLLATP 400
    GKLTNTTYTN TADLTVTPLL GNGTGSFFVL RHSDYSSQAS TPYKLRLPTS 450
    AGQLTIPQLG GSLVLNGRDS KVHLVDYDVA GTKILYSTAE VFTWKKFHDG 500
    KVLVLYGGPG EHHELAVSSK AKVKVVEGLG SGISSKQIRG AVVVAWDVEP 550
    ARRIVQIGDL KIFLLDRNSA YNYWVPQLGT ETSIPYATEK AVAASVIVKA 600
    GYLVRTAYVK GRDLHLTADF NATTPVEVIG APKTAENLFI NGKKAHHTVD 650
    KNGIWSTEVG YSPPKIVLPV LEDLKWKSID TLPEIQPSYD DSPWPDANLP 700
    TKNTIYPLRT PTSLYASDYG FHTGYLLFRG HFTANGRESN FSIQTQGGQA 750
    FGSSVWLSGT YLGSWTGDND YQDYNATYTL PSLKAGKEYV FTVVVDNMGL 800
    NENWIVGQDE MKKPRGILNY ELSGHEASDI TWKLTGNFGG EDYVDKVRGP 850
    LNEGGLYAER HGYHQPYPPT KSKDWKSSTP LTGLSKPGIS FYTASFDLDI 900
    KSGWDVPIYF EFGNSTTPAP AYRVQLYVNG WQYGKYVNNI GPQTRFPVPE 950
    GILNYKGTNW VAVTLWALEG SGAKLDSFKL VHGIPVRTAL DVEGVELPRY 1000
    QSRKGVY 1007
    Length:1,007
    Mass (Da):111,099
    Last modified:July 13, 2010 - v2
    Checksum:i0C80D0D7E3893C4F
    GO

    Sequence cautioni

    The sequence CBF88847.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence EAA65398.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACD01000012 Genomic DNA. Translation: EAA65398.1. Sequence problems.
    BN001308 Genomic DNA. Translation: CBF88847.1. Sequence problems.
    RefSeqiXP_658360.1. XM_653268.1.

    Genome annotation databases

    EnsemblFungiiCADANIAT00001909; CADANIAP00001909; CADANIAG00001909.
    GeneIDi2876531.
    KEGGiani:AN0756.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACD01000012 Genomic DNA. Translation: EAA65398.1 . Sequence problems.
    BN001308 Genomic DNA. Translation: CBF88847.1 . Sequence problems.
    RefSeqi XP_658360.1. XM_653268.1.

    3D structure databases

    ProteinModelPortali Q5BFC4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 162425.CADANIAP00001909.

    Protein family/group databases

    CAZyi GH35. Glycoside Hydrolase Family 35.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADANIAT00001909 ; CADANIAP00001909 ; CADANIAG00001909 .
    GeneIDi 2876531.
    KEGGi ani:AN0756.2.

    Phylogenomic databases

    eggNOGi COG1874.
    HOGENOMi HOG000181922.
    OrthoDBi EOG7ZGXBD.

    Family and domain databases

    Gene3Di 2.102.20.10. 1 hit.
    2.60.120.260. 2 hits.
    2.60.390.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR018954. Betagal_dom2.
    IPR025972. BetaGal_dom3.
    IPR025300. BetaGal_jelly_roll_dom.
    IPR008979. Galactose-bd-like.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR23421. PTHR23421. 1 hit.
    Pfami PF10435. BetaGal_dom2. 1 hit.
    PF13363. BetaGal_dom3. 1 hit.
    PF13364. BetaGal_dom4_5. 2 hits.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view ]
    PRINTSi PR00742. GLHYDRLASE35.
    SMARTi SM01029. BetaGal_dom2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF117100. SSF117100. 1 hit.
    SSF49785. SSF49785. 2 hits.
    SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
    2. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
      Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
      , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
      Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

    Entry informationi

    Entry nameiBGALA_EMENI
    AccessioniPrimary (citable) accession number: Q5BFC4
    Secondary accession number(s): C8VQX7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 13, 2010
    Last sequence update: July 13, 2010
    Last modified: October 1, 2014
    This is version 64 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3