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Q5BFC4

- BGALA_EMENI

UniProt

Q5BFC4 - BGALA_EMENI

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Protein

Probable beta-galactosidase A

Gene

lacA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei96 – 961SubstrateBy similarity
Binding sitei140 – 1401SubstrateBy similarity
Binding sitei141 – 1411Substrate; via amide nitrogenBy similarity
Binding sitei142 – 1421SubstrateBy similarity
Binding sitei199 – 1991SubstrateBy similarity
Active sitei200 – 2001Proton donorSequence Analysis
Binding sitei260 – 2601SubstrateBy similarity
Active sitei298 – 2981NucleophileSequence Analysis
Binding sitei364 – 3641SubstrateBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: ASPGD

GO - Biological processi

  1. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase A (EC:3.2.1.23)
Alternative name(s):
Lactase A
Gene namesi
Name:lacA
ORF Names:AN0756
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000000560: Chromosome VIII

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 1007989Probable beta-galactosidase APRO_0000395220Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi156 – 1561N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi205 ↔ 206By similarity
Disulfide bondi266 ↔ 315By similarity
Glycosylationi405 – 4051N-linked (GlcNAc...)Sequence Analysis
Glycosylationi422 – 4221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi621 – 6211N-linked (GlcNAc...)Sequence Analysis
Glycosylationi740 – 7401N-linked (GlcNAc...)Sequence Analysis
Glycosylationi775 – 7751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi914 – 9141N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi162425.CADANIAP00001909.

Structurei

3D structure databases

ProteinModelPortaliQ5BFC4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000181922.
InParanoidiQ5BFC4.
OrthoDBiEOG7ZGXBD.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5BFC4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRLLPVWTAA LLAAQAAGVA LTHKLNGFTI TEHPDAEKRE LLQKYVTWDD
60 70 80 90 100
KSLFINGERI MIFGAEIHPW RLPVPSLWRD ILQKVKALGF NCVSFYVDWA
110 120 130 140 150
LLEGKPGEYR AEGSFAWEPF FDAASDLGIY LIARPGPYIN AEASGGGFPG
160 170 180 190 200
WLQRLNGTIR SSDQSYLDAT ENYVSHIGGL IAKYQITNGG PVILYQPDNE
210 220 230 240 250
YSGGCCGQEF PNPDYFQYVI DQARRAGIVV PTISNDAWPG GHNAPGTGKG
260 270 280 290 300
EVDIYGHDNY PLGFDCANPD VWPEGNLPTD YRDLHLEISP STPYALVEYQ
310 320 330 340 350
VGAFDPWGGP GFEQCAALTG YEFERVFHKN TFSFGVGILS LYMTFGGTNW
360 370 380 390 400
GNLGHPGGYT SYDYGSPIKE TREITREKYS ELKLLGNFIK SSPGYLLATP
410 420 430 440 450
GKLTNTTYTN TADLTVTPLL GNGTGSFFVL RHSDYSSQAS TPYKLRLPTS
460 470 480 490 500
AGQLTIPQLG GSLVLNGRDS KVHLVDYDVA GTKILYSTAE VFTWKKFHDG
510 520 530 540 550
KVLVLYGGPG EHHELAVSSK AKVKVVEGLG SGISSKQIRG AVVVAWDVEP
560 570 580 590 600
ARRIVQIGDL KIFLLDRNSA YNYWVPQLGT ETSIPYATEK AVAASVIVKA
610 620 630 640 650
GYLVRTAYVK GRDLHLTADF NATTPVEVIG APKTAENLFI NGKKAHHTVD
660 670 680 690 700
KNGIWSTEVG YSPPKIVLPV LEDLKWKSID TLPEIQPSYD DSPWPDANLP
710 720 730 740 750
TKNTIYPLRT PTSLYASDYG FHTGYLLFRG HFTANGRESN FSIQTQGGQA
760 770 780 790 800
FGSSVWLSGT YLGSWTGDND YQDYNATYTL PSLKAGKEYV FTVVVDNMGL
810 820 830 840 850
NENWIVGQDE MKKPRGILNY ELSGHEASDI TWKLTGNFGG EDYVDKVRGP
860 870 880 890 900
LNEGGLYAER HGYHQPYPPT KSKDWKSSTP LTGLSKPGIS FYTASFDLDI
910 920 930 940 950
KSGWDVPIYF EFGNSTTPAP AYRVQLYVNG WQYGKYVNNI GPQTRFPVPE
960 970 980 990 1000
GILNYKGTNW VAVTLWALEG SGAKLDSFKL VHGIPVRTAL DVEGVELPRY

QSRKGVY
Length:1,007
Mass (Da):111,099
Last modified:July 13, 2010 - v2
Checksum:i0C80D0D7E3893C4F
GO

Sequence cautioni

The sequence CBF88847.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence EAA65398.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AACD01000012 Genomic DNA. Translation: EAA65398.1. Sequence problems.
BN001308 Genomic DNA. Translation: CBF88847.1. Sequence problems.
RefSeqiXP_658360.1. XM_653268.1.

Genome annotation databases

EnsemblFungiiCADANIAT00001909; CADANIAP00001909; CADANIAG00001909.
GeneIDi2876531.
KEGGiani:AN0756.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AACD01000012 Genomic DNA. Translation: EAA65398.1 . Sequence problems.
BN001308 Genomic DNA. Translation: CBF88847.1 . Sequence problems.
RefSeqi XP_658360.1. XM_653268.1.

3D structure databases

ProteinModelPortali Q5BFC4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 162425.CADANIAP00001909.

Protein family/group databases

CAZyi GH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADANIAT00001909 ; CADANIAP00001909 ; CADANIAG00001909 .
GeneIDi 2876531.
KEGGi ani:AN0756.2.

Phylogenomic databases

eggNOGi COG1874.
HOGENOMi HOG000181922.
InParanoidi Q5BFC4.
OrthoDBi EOG7ZGXBD.

Family and domain databases

Gene3Di 2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR23421. PTHR23421. 1 hit.
Pfami PF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view ]
PRINTSi PR00742. GLHYDRLASE35.
SMARTi SM01029. BetaGal_dom2. 1 hit.
[Graphical view ]
SUPFAMi SSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  2. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
    Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
    , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
    Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Entry informationi

Entry nameiBGALA_EMENI
AccessioniPrimary (citable) accession number: Q5BFC4
Secondary accession number(s): C8VQX7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: July 13, 2010
Last modified: October 29, 2014
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3