Q5BF93 (MNS1B_EMENI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannosyl-oligosaccharide alpha-1,2-mannosidase 1B
EC=3.2.1.113

Alternative name(s):
Class I alpha-mannosidase 1B
Man(9)-alpha-mannosidase 1B

Gene names

Name:	mns1B
Synonyms:	msdS
ORF Names:	AN0787

Organism

Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)

Taxonomic identifier

227321 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the maturation of Asn-linked oligosaccharides. Progressively trims alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2. Ref.1
Catalytic activity	Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.
Cofactor	Calcium and magnesium divalent cations By similarity.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasmic vesicle lumen By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 47 family.
Sequence caution	The sequence EAA65617.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasmic vesicle
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasmic membrane-bounded vesicle lumen Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 505	489	Mannosyl-oligosaccharide alpha-1,2-mannosidase 1B		PRO_0000394823
Sites
	Active site	368	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	88	1	N-linked (GlcNAc...) Potential
	Glycosylation	174	1	N-linked (GlcNAc...) Potential
	Glycosylation	359	1	N-linked (GlcNAc...) Potential
	Disulfide bond	325 ↔ 354		By similarity
Experimental info
	Sequence conflict	10	1	L → F in AAG48159. Ref.1
	Sequence conflict	420	1	R → L in AAG48159. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5BF93 [UniParc]. Last modified June 15, 2010. Version 2. Checksum: EC77168E8C363B6F	FASTA	505	55,881
	10 20 30 40 50 60 MRTLLALAAL AGFAAARVPA YAITRPVMRS DSRADAVKEA FSHAWDGYYN YAFPHDELHP 70 80 90 100 110 120 ISNGYGDSRN HWGASAVDAL STAIMMRNAT IVNQILDHIA AVDYSKTNAM VSLFETTIRY 130 140 150 160 170 180 LAGMISGYDL LKGPAAGLVD DSRVDVLLEQ SQNLAEVLKF AFDTPSGVPY NMINITSGGN 190 200 210 220 230 240 DGATTNGLAV TGTLVLEWTR LSDLTGNDEY ARLSQRAEDY LLHPEPAQYE PFPGLIGSAV 250 260 270 280 290 300 NIADGKLANG HISWNGGADS YYEYLIKMYV YDPERFGLYR DRWVAAAESS INHLASHPST 310 320 330 340 350 360 RPDVTFLATY NEEHQLGLTS QHLTCFDGGS FLLGGTLLDR QDFVDFGLDL VAGCHETYNS 370 380 390 400 410 420 TLTGIGPEQF SWDPNGVPDS QKELFERAGF YINSGQYILR PEVIESFYYA WRVTGDGTYR 430 440 450 460 470 480 EWVWNAFTNI NKYCRTATGF AGLENVNAAN GGGRIDNQES FMFAEVLKYS FLTFAPEDDW 490 500 QVQKGSGNTF VYNTEAHPFK VYTPQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the class I alpha-mannosidase gene family in the filamentous fungus Aspergillus nidulans." Eades C.J., Hintz W.E. Gene 255:25-34(2000) [PubMed: 10974561] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: SM222.
[2]	"Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls." Bauer S., Vasu P., Persson S., Mort A.J., Somerville C.R. Proc. Natl. Acad. Sci. U.S.A. 103:11417-11422(2006) [PubMed: 16844780] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[3]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed: 16372000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[4]	"The 2008 update of the Aspergillus nidulans genome annotation: a community effort." Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G. , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G. Fungal Genet. Biol. 46:S2-13(2009) [PubMed: 19146970] [Abstract] Cited for: GENOME REANNOTATION. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF129496 Genomic DNA. Translation: AAG48159.1. DQ490469 mRNA. Translation: ABF50845.1. AACD01000013 Genomic DNA. Translation: EAA65617.1. Sequence problems. BN001308 Genomic DNA. Translation: CBF88785.1.
RefSeq	XP_658391.1. XM_653299.1.
3D structure databases
HSSP	HSSP built from PDB template 1KRE based on UniProtKB P31723.
ProteinModelPortal	Q5BF93.
SMR	Q5BF93. Positions 30-501.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5BF93.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2876566.
KEGG	ani:AN0787.2.
Phylogenomic databases
OMA	QILDHIA.
OrthoDB	EOG42JS14.
PhylomeDB	Q5BF93.
Family and domain databases
InterPro	IPR001382. Glyco_hydro_47. [Graphical view]
Gene3D	G3DSA:1.50.10.50. Glyco_hydro_47. 1 hit.
KO	K01230.
PANTHER	PTHR11742. Glyco_hydro_47. 1 hit.
Pfam	PF01532. Glyco_hydro_47. 1 hit. [Graphical view]
PRINTS	PR00747. GLYHDRLASE47.
SUPFAM	SSF48225. Glyco_hydro_47. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MNS1B_EMENI

Accession

Primary (citable) accession number: Q5BF93
Secondary accession number(s): C8VQU4, Q1HFV5, Q9HF85

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	June 15, 2010
Last modified:	January 25, 2012
This is version 38 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program