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Protein

Probable beta-galactosidase B

Gene

lacB

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei90SubstrateBy similarity1
Binding sitei135SubstrateBy similarity1
Binding sitei136Substrate; via amide nitrogenBy similarity1
Binding sitei137SubstrateBy similarity1
Binding sitei195SubstrateBy similarity1
Active sitei196Proton donorSequence analysis1
Binding sitei265SubstrateBy similarity1
Active sitei308NucleophileSequence analysis1
Binding sitei373SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase B (EC:3.2.1.23)
Alternative name(s):
Lactase B
Gene namesi
Name:lacB
ORF Names:AN0980
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome VIII
  • UP000005890 Componenti: Partially assembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000039522922 – 1025Probable beta-galactosidase BAdd BLAST1004

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi23N-linked (GlcNAc...)Sequence analysis1
Glycosylationi100N-linked (GlcNAc...)Sequence analysis1
Glycosylationi211N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi271 ↔ 324By similarity
Glycosylationi411N-linked (GlcNAc...)Sequence analysis1
Glycosylationi456N-linked (GlcNAc...)Sequence analysis1
Glycosylationi736N-linked (GlcNAc...)Sequence analysis1
Glycosylationi776N-linked (GlcNAc...)Sequence analysis1
Glycosylationi884N-linked (GlcNAc...)Sequence analysis1
Glycosylationi925N-linked (GlcNAc...)Sequence analysis1
Glycosylationi926N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ5BEQ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000181922.
InParanoidiQ5BEQ0.
OrthoDBiEOG092C0SLI.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 2 hits.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5BEQ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATAFWLLLF LLGSLHVLTA AQNSSQSEWP IHDNGLSKVV QWDHYSFYIN
60 70 80 90 100
GQRIFLFSGE FHYWRIPVPA LWRDILEKIK AIGFTGFAFY SSWAYHAPNN
110 120 130 140 150
QTVDFSTGAR DITPIYDLAK ELGMYIIVRP GPYVNAEASA GGFPLWLTTG
160 170 180 190 200
AYGSTRNDDP RYTAAWEPYF AEVSEITSKY QVTDGHYTLC YQIENEYGQQ
210 220 230 240 250
WIGDPRDRNP NQTAIAYMEL LQASARENGI TVPLTGNDPN MNTKSWGSDW
260 270 280 290 300
SDAGGNLDTV GLDSYPSCWS CDVSVCTGTN GEYVPYKVLD YYDYFQEVQP
310 320 330 340 350
TMPFFMPEFQ GGSYNPWDGP EGGCTEDTGA DFANLFYRWN IGQRVSAMSL
360 370 380 390 400
YMMFGGTNWG GIAAPVTASS YDYSAPISED RSIGSKYYET KLLALFTRCA
410 420 430 440 450
KDLTMTDRLG NGTQYTDNEA VIASELRNPD TNAAFYVTTH LDTTVGTDES
460 470 480 490 500
FKLHVNTSKG ALTIPRHGGT IRLNGHHSKI IVTDFNFGSE TLLYSTAEVL
510 520 530 540 550
TYAVFDRKPT LVLWVPTGES GEFAIKGAKS GSVAKCSGCS NIKFHRDSGS
560 570 580 590 600
LTVAFTQGEG ISVLQLDNGV RVVLLDRQKA YTFWAPALTD NPLVPEGESV
610 620 630 640 650
LVSGPYLVRT ARLARSTLTL RGDSKGETLE IFAPRKIKKV TWNGKAVEAT
660 670 680 690 700
RTSYGSLKAI LAKPPSVELP TLNGWKYSDS LPERFPTYDD SGAAWVEIDA
710 720 730 740 750
NHMTTPNPNK PATLPVLYAD EYGFHNGVRL WRGYFNSSAS GVYLNIQGGA
760 770 780 790 800
AFGWSAWLNG HFLGSHLGSA SIQQANGTLD FPANTLNTEG TPNVLLVVHD
810 820 830 840 850
DTGHDQTTGV LNPRGILEAR LLSEASDNND DDSPGFTHWR VAGTAGGESD
860 870 880 890 900
LDPVRGVYNE DGLYAERVGW HLPGFDDSKW ATVNGTSLSF TGATVRFFRT
910 920 930 940 950
VIPPLSIPEN TDVSISFVFS TPNVNNTSAG NTSAFRAQLF VNGYQYGRYN
960 970 980 990 1000
PYVGNQVVYP VPPGILDYNG ENTIGVAVWA QTEAGARLNL DWRVNYVLGS
1010 1020
SLDAGRLDLS FVAIAYVYIF ECLQL
Length:1,025
Mass (Da):112,791
Last modified:July 13, 2010 - v2
Checksum:i042F7E48B07F2BD4
GO

Sequence cautioni

The sequence CBF88404 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence EAA66009 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACD01000014 Genomic DNA. Translation: EAA66009.1. Sequence problems.
BN001308 Genomic DNA. Translation: CBF88404.1. Sequence problems.
RefSeqiXP_658584.1. XM_653492.1.

Genome annotation databases

EnsemblFungiiCADANIAT00001672; CADANIAP00001672; CADANIAG00001672.
EAA66009; EAA66009; AN0980.2.
GeneIDi2876757.
KEGGiani:AN0980.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACD01000014 Genomic DNA. Translation: EAA66009.1. Sequence problems.
BN001308 Genomic DNA. Translation: CBF88404.1. Sequence problems.
RefSeqiXP_658584.1. XM_653492.1.

3D structure databases

ProteinModelPortaliQ5BEQ0.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANIAT00001672; CADANIAP00001672; CADANIAG00001672.
EAA66009; EAA66009; AN0980.2.
GeneIDi2876757.
KEGGiani:AN0980.2.

Phylogenomic databases

HOGENOMiHOG000181922.
InParanoidiQ5BEQ0.
OrthoDBiEOG092C0SLI.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR031330. Gly_Hdrlase_35_cat.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 2 hits.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBGALB_EMENI
AccessioniPrimary (citable) accession number: Q5BEQ0
Secondary accession number(s): C8VU89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: July 13, 2010
Last modified: September 7, 2016
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.