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Q5BEQ0 (BGALB_EMENI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable beta-galactosidase B

EC=3.2.1.23
Alternative name(s):
Lactase B
Gene names
Name:lacB
ORF Names:AN0980
OrganismEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) [Reference proteome]
Taxonomic identifier227321 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length1025 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans By similarity.

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Subcellular location

Secreted By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 35 family.

Sequence caution

The sequence CBF88404.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence EAA66009.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Polysaccharide degradation
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpolysaccharide catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-galactosidase activity

Inferred from direct assay PubMed 24037406. Source: ASPGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 10251004Probable beta-galactosidase B
PRO_0000395229

Sites

Active site1961Proton donor Potential
Active site3081Nucleophile Potential
Binding site901Substrate By similarity
Binding site1351Substrate By similarity
Binding site1361Substrate; via amide nitrogen By similarity
Binding site1371Substrate By similarity
Binding site1951Substrate By similarity
Binding site2651Substrate By similarity
Binding site3731Substrate By similarity

Amino acid modifications

Glycosylation231N-linked (GlcNAc...) Potential
Glycosylation1001N-linked (GlcNAc...) Potential
Glycosylation2111N-linked (GlcNAc...) Potential
Glycosylation4111N-linked (GlcNAc...) Potential
Glycosylation4561N-linked (GlcNAc...) Potential
Glycosylation7361N-linked (GlcNAc...) Potential
Glycosylation7761N-linked (GlcNAc...) Potential
Glycosylation8841N-linked (GlcNAc...) Potential
Glycosylation9251N-linked (GlcNAc...) Potential
Glycosylation9261N-linked (GlcNAc...) Potential
Disulfide bond271 ↔ 324 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5BEQ0 [UniParc].

Last modified July 13, 2010. Version 2.
Checksum: 042F7E48B07F2BD4

FASTA1,025112,791
        10         20         30         40         50         60 
MATAFWLLLF LLGSLHVLTA AQNSSQSEWP IHDNGLSKVV QWDHYSFYIN GQRIFLFSGE 

        70         80         90        100        110        120 
FHYWRIPVPA LWRDILEKIK AIGFTGFAFY SSWAYHAPNN QTVDFSTGAR DITPIYDLAK 

       130        140        150        160        170        180 
ELGMYIIVRP GPYVNAEASA GGFPLWLTTG AYGSTRNDDP RYTAAWEPYF AEVSEITSKY 

       190        200        210        220        230        240 
QVTDGHYTLC YQIENEYGQQ WIGDPRDRNP NQTAIAYMEL LQASARENGI TVPLTGNDPN 

       250        260        270        280        290        300 
MNTKSWGSDW SDAGGNLDTV GLDSYPSCWS CDVSVCTGTN GEYVPYKVLD YYDYFQEVQP 

       310        320        330        340        350        360 
TMPFFMPEFQ GGSYNPWDGP EGGCTEDTGA DFANLFYRWN IGQRVSAMSL YMMFGGTNWG 

       370        380        390        400        410        420 
GIAAPVTASS YDYSAPISED RSIGSKYYET KLLALFTRCA KDLTMTDRLG NGTQYTDNEA 

       430        440        450        460        470        480 
VIASELRNPD TNAAFYVTTH LDTTVGTDES FKLHVNTSKG ALTIPRHGGT IRLNGHHSKI 

       490        500        510        520        530        540 
IVTDFNFGSE TLLYSTAEVL TYAVFDRKPT LVLWVPTGES GEFAIKGAKS GSVAKCSGCS 

       550        560        570        580        590        600 
NIKFHRDSGS LTVAFTQGEG ISVLQLDNGV RVVLLDRQKA YTFWAPALTD NPLVPEGESV 

       610        620        630        640        650        660 
LVSGPYLVRT ARLARSTLTL RGDSKGETLE IFAPRKIKKV TWNGKAVEAT RTSYGSLKAI 

       670        680        690        700        710        720 
LAKPPSVELP TLNGWKYSDS LPERFPTYDD SGAAWVEIDA NHMTTPNPNK PATLPVLYAD 

       730        740        750        760        770        780 
EYGFHNGVRL WRGYFNSSAS GVYLNIQGGA AFGWSAWLNG HFLGSHLGSA SIQQANGTLD 

       790        800        810        820        830        840 
FPANTLNTEG TPNVLLVVHD DTGHDQTTGV LNPRGILEAR LLSEASDNND DDSPGFTHWR 

       850        860        870        880        890        900 
VAGTAGGESD LDPVRGVYNE DGLYAERVGW HLPGFDDSKW ATVNGTSLSF TGATVRFFRT 

       910        920        930        940        950        960 
VIPPLSIPEN TDVSISFVFS TPNVNNTSAG NTSAFRAQLF VNGYQYGRYN PYVGNQVVYP 

       970        980        990       1000       1010       1020 
VPPGILDYNG ENTIGVAVWA QTEAGARLNL DWRVNYVLGS SLDAGRLDLS FVAIAYVYIF 


ECLQL 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae."
Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. expand/collapse author list , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.
Nature 438:1105-1115(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[2]"The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G. expand/collapse author list , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AACD01000014 Genomic DNA. Translation: EAA66009.1. Sequence problems.
BN001308 Genomic DNA. Translation: CBF88404.1. Sequence problems.
RefSeqXP_658584.1. XM_653492.1.

3D structure databases

ProteinModelPortalQ5BEQ0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING162425.CADANIAP00001672.

Protein family/group databases

CAZyGH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADANIAT00001672; CADANIAP00001672; CADANIAG00001672.
GeneID2876757.
KEGGani:AN0980.2.

Phylogenomic databases

eggNOGCOG1874.
HOGENOMHOG000181922.
OrthoDBEOG7ZGXBD.

Family and domain databases

Gene3D2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR23421. PTHR23421. 1 hit.
PfamPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSPR00742. GLHYDRLASE35.
SMARTSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBGALB_EMENI
AccessionPrimary (citable) accession number: Q5BEQ0
Secondary accession number(s): C8VU89
Entry history
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: July 13, 2010
Last modified: April 16, 2014
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries