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Q5BEQ0

- BGALB_EMENI

UniProt

Q5BEQ0 - BGALB_EMENI

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Protein

Probable beta-galactosidase B

Gene

lacB

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei90 – 901SubstrateBy similarity
Binding sitei135 – 1351SubstrateBy similarity
Binding sitei136 – 1361Substrate; via amide nitrogenBy similarity
Binding sitei137 – 1371SubstrateBy similarity
Binding sitei195 – 1951SubstrateBy similarity
Active sitei196 – 1961Proton donorSequence Analysis
Binding sitei265 – 2651SubstrateBy similarity
Active sitei308 – 3081NucleophileSequence Analysis
Binding sitei373 – 3731SubstrateBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: ASPGD

GO - Biological processi

  1. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Polysaccharide degradation

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-galactosidase B (EC:3.2.1.23)
Alternative name(s):
Lactase B
Gene namesi
Name:lacB
ORF Names:AN0980
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000000560: Chromosome VIII

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence AnalysisAdd
BLAST
Chaini22 – 10251004Probable beta-galactosidase BPRO_0000395229Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi23 – 231N-linked (GlcNAc...)Sequence Analysis
Glycosylationi100 – 1001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi211 – 2111N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi271 ↔ 324By similarity
Glycosylationi411 – 4111N-linked (GlcNAc...)Sequence Analysis
Glycosylationi456 – 4561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi736 – 7361N-linked (GlcNAc...)Sequence Analysis
Glycosylationi776 – 7761N-linked (GlcNAc...)Sequence Analysis
Glycosylationi884 – 8841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi925 – 9251N-linked (GlcNAc...)Sequence Analysis
Glycosylationi926 – 9261N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi162425.CADANIAP00001672.

Structurei

3D structure databases

ProteinModelPortaliQ5BEQ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
HOGENOMiHOG000181922.
InParanoidiQ5BEQ0.
OrthoDBiEOG7ZGXBD.

Family and domain databases

Gene3Di2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PRINTSiPR00742. GLHYDRLASE35.
SMARTiSM01029. BetaGal_dom2. 1 hit.
[Graphical view]
SUPFAMiSSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5BEQ0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATAFWLLLF LLGSLHVLTA AQNSSQSEWP IHDNGLSKVV QWDHYSFYIN
60 70 80 90 100
GQRIFLFSGE FHYWRIPVPA LWRDILEKIK AIGFTGFAFY SSWAYHAPNN
110 120 130 140 150
QTVDFSTGAR DITPIYDLAK ELGMYIIVRP GPYVNAEASA GGFPLWLTTG
160 170 180 190 200
AYGSTRNDDP RYTAAWEPYF AEVSEITSKY QVTDGHYTLC YQIENEYGQQ
210 220 230 240 250
WIGDPRDRNP NQTAIAYMEL LQASARENGI TVPLTGNDPN MNTKSWGSDW
260 270 280 290 300
SDAGGNLDTV GLDSYPSCWS CDVSVCTGTN GEYVPYKVLD YYDYFQEVQP
310 320 330 340 350
TMPFFMPEFQ GGSYNPWDGP EGGCTEDTGA DFANLFYRWN IGQRVSAMSL
360 370 380 390 400
YMMFGGTNWG GIAAPVTASS YDYSAPISED RSIGSKYYET KLLALFTRCA
410 420 430 440 450
KDLTMTDRLG NGTQYTDNEA VIASELRNPD TNAAFYVTTH LDTTVGTDES
460 470 480 490 500
FKLHVNTSKG ALTIPRHGGT IRLNGHHSKI IVTDFNFGSE TLLYSTAEVL
510 520 530 540 550
TYAVFDRKPT LVLWVPTGES GEFAIKGAKS GSVAKCSGCS NIKFHRDSGS
560 570 580 590 600
LTVAFTQGEG ISVLQLDNGV RVVLLDRQKA YTFWAPALTD NPLVPEGESV
610 620 630 640 650
LVSGPYLVRT ARLARSTLTL RGDSKGETLE IFAPRKIKKV TWNGKAVEAT
660 670 680 690 700
RTSYGSLKAI LAKPPSVELP TLNGWKYSDS LPERFPTYDD SGAAWVEIDA
710 720 730 740 750
NHMTTPNPNK PATLPVLYAD EYGFHNGVRL WRGYFNSSAS GVYLNIQGGA
760 770 780 790 800
AFGWSAWLNG HFLGSHLGSA SIQQANGTLD FPANTLNTEG TPNVLLVVHD
810 820 830 840 850
DTGHDQTTGV LNPRGILEAR LLSEASDNND DDSPGFTHWR VAGTAGGESD
860 870 880 890 900
LDPVRGVYNE DGLYAERVGW HLPGFDDSKW ATVNGTSLSF TGATVRFFRT
910 920 930 940 950
VIPPLSIPEN TDVSISFVFS TPNVNNTSAG NTSAFRAQLF VNGYQYGRYN
960 970 980 990 1000
PYVGNQVVYP VPPGILDYNG ENTIGVAVWA QTEAGARLNL DWRVNYVLGS
1010 1020
SLDAGRLDLS FVAIAYVYIF ECLQL
Length:1,025
Mass (Da):112,791
Last modified:July 13, 2010 - v2
Checksum:i042F7E48B07F2BD4
GO

Sequence cautioni

The sequence CBF88404.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence EAA66009.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACD01000014 Genomic DNA. Translation: EAA66009.1. Sequence problems.
BN001308 Genomic DNA. Translation: CBF88404.1. Sequence problems.
RefSeqiXP_658584.1. XM_653492.1.

Genome annotation databases

EnsemblFungiiCADANIAT00001672; CADANIAP00001672; CADANIAG00001672.
GeneIDi2876757.
KEGGiani:AN0980.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACD01000014 Genomic DNA. Translation: EAA66009.1 . Sequence problems.
BN001308 Genomic DNA. Translation: CBF88404.1 . Sequence problems.
RefSeqi XP_658584.1. XM_653492.1.

3D structure databases

ProteinModelPortali Q5BEQ0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 162425.CADANIAP00001672.

Protein family/group databases

CAZyi GH35. Glycoside Hydrolase Family 35.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CADANIAT00001672 ; CADANIAP00001672 ; CADANIAG00001672 .
GeneIDi 2876757.
KEGGi ani:AN0980.2.

Phylogenomic databases

eggNOGi COG1874.
HOGENOMi HOG000181922.
InParanoidi Q5BEQ0.
OrthoDBi EOG7ZGXBD.

Family and domain databases

Gene3Di 2.102.20.10. 1 hit.
2.60.120.260. 2 hits.
2.60.390.10. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR018954. Betagal_dom2.
IPR025972. BetaGal_dom3.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR23421. PTHR23421. 1 hit.
Pfami PF10435. BetaGal_dom2. 1 hit.
PF13363. BetaGal_dom3. 1 hit.
PF13364. BetaGal_dom4_5. 2 hits.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view ]
PRINTSi PR00742. GLHYDRLASE35.
SMARTi SM01029. BetaGal_dom2. 1 hit.
[Graphical view ]
SUPFAMi SSF117100. SSF117100. 1 hit.
SSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  2. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
    Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
    , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
    Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Entry informationi

Entry nameiBGALB_EMENI
AccessioniPrimary (citable) accession number: Q5BEQ0
Secondary accession number(s): C8VU89
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: July 13, 2010
Last modified: October 29, 2014
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3