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Q5BEQ0

- BGALB_EMENI

UniProt

Q5BEQ0 - BGALB_EMENI

Protein

Probable beta-galactosidase B

Gene

lacB

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 2 (13 Jul 2010)
      Previous versions | rss
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    Functioni

    Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei90 – 901SubstrateBy similarity
    Binding sitei135 – 1351SubstrateBy similarity
    Binding sitei136 – 1361Substrate; via amide nitrogenBy similarity
    Binding sitei137 – 1371SubstrateBy similarity
    Binding sitei195 – 1951SubstrateBy similarity
    Active sitei196 – 1961Proton donorSequence Analysis
    Binding sitei265 – 2651SubstrateBy similarity
    Active sitei308 – 3081NucleophileSequence Analysis
    Binding sitei373 – 3731SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: ASPGD

    GO - Biological processi

    1. polysaccharide catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation

    Protein family/group databases

    CAZyiGH35. Glycoside Hydrolase Family 35.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable beta-galactosidase B (EC:3.2.1.23)
    Alternative name(s):
    Lactase B
    Gene namesi
    Name:lacB
    ORF Names:AN0980
    OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
    Taxonomic identifieri227321 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000000560: Chromosome VIII

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 10251004Probable beta-galactosidase BPRO_0000395229Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi23 – 231N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi100 – 1001N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi211 – 2111N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi271 ↔ 324By similarity
    Glycosylationi411 – 4111N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi456 – 4561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi736 – 7361N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi776 – 7761N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi884 – 8841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi925 – 9251N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi926 – 9261N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi162425.CADANIAP00001672.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5BEQ0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 35 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1874.
    HOGENOMiHOG000181922.
    OrthoDBiEOG7ZGXBD.

    Family and domain databases

    Gene3Di2.102.20.10. 1 hit.
    2.60.120.260. 2 hits.
    2.60.390.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR018954. Betagal_dom2.
    IPR025972. BetaGal_dom3.
    IPR025300. BetaGal_jelly_roll_dom.
    IPR008979. Galactose-bd-like.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR23421. PTHR23421. 1 hit.
    PfamiPF10435. BetaGal_dom2. 1 hit.
    PF13363. BetaGal_dom3. 1 hit.
    PF13364. BetaGal_dom4_5. 2 hits.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view]
    PRINTSiPR00742. GLHYDRLASE35.
    SMARTiSM01029. BetaGal_dom2. 1 hit.
    [Graphical view]
    SUPFAMiSSF117100. SSF117100. 1 hit.
    SSF49785. SSF49785. 2 hits.
    SSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5BEQ0-1 [UniParc]FASTAAdd to Basket

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    MATAFWLLLF LLGSLHVLTA AQNSSQSEWP IHDNGLSKVV QWDHYSFYIN     50
    GQRIFLFSGE FHYWRIPVPA LWRDILEKIK AIGFTGFAFY SSWAYHAPNN 100
    QTVDFSTGAR DITPIYDLAK ELGMYIIVRP GPYVNAEASA GGFPLWLTTG 150
    AYGSTRNDDP RYTAAWEPYF AEVSEITSKY QVTDGHYTLC YQIENEYGQQ 200
    WIGDPRDRNP NQTAIAYMEL LQASARENGI TVPLTGNDPN MNTKSWGSDW 250
    SDAGGNLDTV GLDSYPSCWS CDVSVCTGTN GEYVPYKVLD YYDYFQEVQP 300
    TMPFFMPEFQ GGSYNPWDGP EGGCTEDTGA DFANLFYRWN IGQRVSAMSL 350
    YMMFGGTNWG GIAAPVTASS YDYSAPISED RSIGSKYYET KLLALFTRCA 400
    KDLTMTDRLG NGTQYTDNEA VIASELRNPD TNAAFYVTTH LDTTVGTDES 450
    FKLHVNTSKG ALTIPRHGGT IRLNGHHSKI IVTDFNFGSE TLLYSTAEVL 500
    TYAVFDRKPT LVLWVPTGES GEFAIKGAKS GSVAKCSGCS NIKFHRDSGS 550
    LTVAFTQGEG ISVLQLDNGV RVVLLDRQKA YTFWAPALTD NPLVPEGESV 600
    LVSGPYLVRT ARLARSTLTL RGDSKGETLE IFAPRKIKKV TWNGKAVEAT 650
    RTSYGSLKAI LAKPPSVELP TLNGWKYSDS LPERFPTYDD SGAAWVEIDA 700
    NHMTTPNPNK PATLPVLYAD EYGFHNGVRL WRGYFNSSAS GVYLNIQGGA 750
    AFGWSAWLNG HFLGSHLGSA SIQQANGTLD FPANTLNTEG TPNVLLVVHD 800
    DTGHDQTTGV LNPRGILEAR LLSEASDNND DDSPGFTHWR VAGTAGGESD 850
    LDPVRGVYNE DGLYAERVGW HLPGFDDSKW ATVNGTSLSF TGATVRFFRT 900
    VIPPLSIPEN TDVSISFVFS TPNVNNTSAG NTSAFRAQLF VNGYQYGRYN 950
    PYVGNQVVYP VPPGILDYNG ENTIGVAVWA QTEAGARLNL DWRVNYVLGS 1000
    SLDAGRLDLS FVAIAYVYIF ECLQL 1025
    Length:1,025
    Mass (Da):112,791
    Last modified:July 13, 2010 - v2
    Checksum:i042F7E48B07F2BD4
    GO

    Sequence cautioni

    The sequence CBF88404.1 differs from that shown. Reason: Erroneous gene model prediction.
    The sequence EAA66009.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACD01000014 Genomic DNA. Translation: EAA66009.1. Sequence problems.
    BN001308 Genomic DNA. Translation: CBF88404.1. Sequence problems.
    RefSeqiXP_658584.1. XM_653492.1.

    Genome annotation databases

    EnsemblFungiiCADANIAT00001672; CADANIAP00001672; CADANIAG00001672.
    GeneIDi2876757.
    KEGGiani:AN0980.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACD01000014 Genomic DNA. Translation: EAA66009.1 . Sequence problems.
    BN001308 Genomic DNA. Translation: CBF88404.1 . Sequence problems.
    RefSeqi XP_658584.1. XM_653492.1.

    3D structure databases

    ProteinModelPortali Q5BEQ0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 162425.CADANIAP00001672.

    Protein family/group databases

    CAZyi GH35. Glycoside Hydrolase Family 35.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADANIAT00001672 ; CADANIAP00001672 ; CADANIAG00001672 .
    GeneIDi 2876757.
    KEGGi ani:AN0980.2.

    Phylogenomic databases

    eggNOGi COG1874.
    HOGENOMi HOG000181922.
    OrthoDBi EOG7ZGXBD.

    Family and domain databases

    Gene3Di 2.102.20.10. 1 hit.
    2.60.120.260. 2 hits.
    2.60.390.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR018954. Betagal_dom2.
    IPR025972. BetaGal_dom3.
    IPR025300. BetaGal_jelly_roll_dom.
    IPR008979. Galactose-bd-like.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR001944. Glycoside_Hdrlase_35.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR23421. PTHR23421. 1 hit.
    Pfami PF10435. BetaGal_dom2. 1 hit.
    PF13363. BetaGal_dom3. 1 hit.
    PF13364. BetaGal_dom4_5. 2 hits.
    PF01301. Glyco_hydro_35. 1 hit.
    [Graphical view ]
    PRINTSi PR00742. GLHYDRLASE35.
    SMARTi SM01029. BetaGal_dom2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF117100. SSF117100. 1 hit.
    SSF49785. SSF49785. 2 hits.
    SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
    2. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
      Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
      , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
      Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

    Entry informationi

    Entry nameiBGALB_EMENI
    AccessioniPrimary (citable) accession number: Q5BEQ0
    Secondary accession number(s): C8VU89
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 13, 2010
    Last sequence update: July 13, 2010
    Last modified: October 1, 2014
    This is version 64 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3