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Q5BDU5

- EGLA_EMENI

UniProt

Q5BDU5 - EGLA_EMENI

Protein

Endo-beta-1,4-glucanase A

Gene

eglA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (26 Apr 2005)
      Previous versions | rss
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    Functioni

    Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates.2 Publications

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    pH dependencei

    Optimum pH is 4.0.1 Publication

    Temperature dependencei

    Optimum temperature is 57 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei150 – 1501Proton donorBy similarity
    Active sitei257 – 2571NucleophileBy similarity

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW
    2. glucan catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Protein family/group databases

    mycoCLAPiEGL5A_EMENI.
    EGL5B_EMENI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endo-beta-1,4-glucanase A (EC:3.2.1.4)
    Short name:
    Endoglucanase A
    Alternative name(s):
    Carboxymethylcellulase A
    Cellulase A
    Gene namesi
    Name:eglA
    ORF Names:AN1285
    OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
    Taxonomic identifieri227321 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    ProteomesiUP000000560: Chromosome VIII

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 326307Endo-beta-1,4-glucanase APRO_5000049150Add
    BLAST

    Expressioni

    Inductioni

    Highly expressed in presence of carboxymethylcellulose (CMC).1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ5BDU5.
    SMRiQ5BDU5. Positions 21-322.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2730.
    HOGENOMiHOG000111120.
    KOiK01179.
    OMAiYHYPAAD.
    OrthoDBiEOG776T0S.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001547. Glyco_hydro_5.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00150. Cellulase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5BDU5-1 [UniParc]FASTAAdd to Basket

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    MRSLVLLSSV LALVAPSKGA FTWLGTNEAG AEFGEGSYPG ELGTEYIWPD    50
    LGTIGTLRNE GMNIFRVAFS MERLVPDSLA GPVADEYFQD LVETVNGITA 100
    LGAYAVLDPH NYGRYYGNII TSTDDFAAFW TILATEFASN ELVIFDTNNE 150
    YHTMDQSLVL NLNQAAIDAI RASGATSQYI FAEGNSWTGA WTWVDVNDNM 200
    KALTDPQDKL IYEMHQYLDS DGSGTNTACV SSTIGSERVT AATNWLRENG 250
    KLGVLGEFAG ANNQVCKDAV ADLLEYLEEN SDVWLGALWW AAGPWWGDYM 300
    FNMEPTSGIA YQEYSEILQP YFVGSQ 326
    Length:326
    Mass (Da):35,812
    Last modified:April 26, 2005 - v1
    Checksum:i7BFED0EF254B8D9F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti116 – 1161Missing in BAA82592. (PubMed:10386374)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB009402 Genomic DNA. Translation: BAA82592.1.
    DQ490472 mRNA. Translation: ABF50848.1.
    AACD01000017 Genomic DNA. Translation: EAA65878.1.
    BN001308 Genomic DNA. Translation: CBF87793.1.
    RefSeqiXP_658889.1. XM_653797.1.

    Genome annotation databases

    EnsemblFungiiCADANIAT00001335; CADANIAP00001335; CADANIAG00001335.
    GeneIDi2877061.
    KEGGiani:AN1285.2.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB009402 Genomic DNA. Translation: BAA82592.1 .
    DQ490472 mRNA. Translation: ABF50848.1 .
    AACD01000017 Genomic DNA. Translation: EAA65878.1 .
    BN001308 Genomic DNA. Translation: CBF87793.1 .
    RefSeqi XP_658889.1. XM_653797.1.

    3D structure databases

    ProteinModelPortali Q5BDU5.
    SMRi Q5BDU5. Positions 21-322.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    mycoCLAPi EGL5A_EMENI.
    EGL5B_EMENI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CADANIAT00001335 ; CADANIAP00001335 ; CADANIAG00001335 .
    GeneIDi 2877061.
    KEGGi ani:AN1285.2.

    Phylogenomic databases

    eggNOGi COG2730.
    HOGENOMi HOG000111120.
    KOi K01179.
    OMAi YHYPAAD.
    OrthoDBi EOG776T0S.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001547. Glyco_hydro_5.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00150. Cellulase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Structure and expression properties of the endo-beta-1,4-glucanase A gene from the filamentous fungus Aspergillus nidulans."
      Chikamatsu G., Shirai K., Kato M., Kobayashi T., Tsukagoshi N.
      FEMS Microbiol. Lett. 175:239-245(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION.
    2. "Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls."
      Bauer S., Vasu P., Persson S., Mort A.J., Somerville C.R.
      Proc. Natl. Acad. Sci. U.S.A. 103:11417-11422(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
    4. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
      Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
      , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
      Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

    Entry informationi

    Entry nameiEGLA_EMENI
    AccessioniPrimary (citable) accession number: Q5BDU5
    Secondary accession number(s): C8VSF3, Q1HFV2, Q9Y8H6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 18, 2010
    Last sequence update: April 26, 2005
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3