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Protein

Probable beta-glucosidase btgE

Gene

btgE

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Beta-glucosidases are one of a number of cellulolytic enzymes involved in the degradation of cellulosic biomass. Catalyzes the last step releasing glucose from the inhibitory cellobiose (By similarity).By similarity

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei488 – 4881NucleophileBy similarity
Active sitei546 – 5461Proton donorBy similarity

GO - Molecular functioni

  1. beta-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Enzyme and pathway databases

UniPathwayiUPA00696.

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable beta-glucosidase btgE (EC:3.2.1.21)
Alternative name(s):
Beta-D-glucoside glucohydrolase btgE
Cellobiase btgE
Gentiobiase btgE
Gene namesi
Name:btgE
ORF Names:AN1551
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000000560 Componenti: Chromosome VII

Subcellular locationi

Secretedcell wall 1 Publication
Note: Covalently-linked to the cell wall.

GO - Cellular componenti

  1. cell wall Source: UniProtKB-SubCell
  2. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 555537Probable beta-glucosidase btgEPRO_0000395136Add
BLAST

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi49 – 288240Thr-richAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5309.
HOGENOMiHOG000158427.
InParanoidiQ5BD29.
OMAiYSTDCDT.
OrthoDBiEOG73FQWG.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5BD29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGAILATAA AFAGTAVADM HMRRHAHEGL HHRALHASSA VPEEECGCTT
60 70 80 90 100
EVITYWGEPT TIPLSVPTST VTSETTETVH STSYSTVTVT ATSSAAPVET
110 120 130 140 150
PSETPSPTPE VTLPTAGVTS YSETGTYTIP ATTITVTDTT TVCGATTTEL
160 170 180 190 200
PSGTHTYGGV TTIVETATTI TCPYATVKPT GSTVTSVIET TTYVCPSAGT
210 220 230 240 250
YTIAPTTTFV PTSTVVVYPT PETVTPGTYT NPGTTITVTR TEDVYVCPYT
260 270 280 290 300
NGNVPTSVPA LPTTSAASTT TAVPSSSTTT SSATSVPTGA SGNKMGMTFT
310 320 330 340 350
PYNNDGSCMA KNDVLEQVGL IKGKGFSHVR VYGTDCHTLE YVGAACSTHG
360 370 380 390 400
LKMILGVNVE GSTGFDGARS QFKDITNWGQ WDLVSLIVVG NEVVTSNIAS
410 420 430 440 450
AAQLASFVSE GASAFSAAGY TGQVTTAEPI DVWLSNGATL CPVVDILGAN
460 470 480 490 500
LHPFFNPEFT AAEAGTLVSN QIKDLKQVCT GKDVINLETG WPNAGSANGK
510 520 530 540 550
AIPGQSQQTT AIKSLVEKVG DVSVFFSYAD DGWKSKFATS DKYNVEQHWG

CIDQF
Length:555
Mass (Da):57,672
Last modified:April 26, 2005 - v1
Checksum:iB8F0DF0153079DCA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ490473 mRNA. Translation: ABF50849.1.
AACD01000025 Genomic DNA. Translation: EAA64258.1.
BN001307 Genomic DNA. Translation: CBF85093.1.
RefSeqiXP_659155.1. XM_654063.1.

Genome annotation databases

EnsemblFungiiCADANIAT00008179; CADANIAP00008179; CADANIAG00008179.
GeneIDi2874885.
KEGGiani:AN1551.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ490473 mRNA. Translation: ABF50849.1.
AACD01000025 Genomic DNA. Translation: EAA64258.1.
BN001307 Genomic DNA. Translation: CBF85093.1.
RefSeqiXP_659155.1. XM_654063.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANIAT00008179; CADANIAP00008179; CADANIAG00008179.
GeneIDi2874885.
KEGGiani:AN1551.2.

Phylogenomic databases

eggNOGiCOG5309.
HOGENOMiHOG000158427.
InParanoidiQ5BD29.
OMAiYSTDCDT.
OrthoDBiEOG73FQWG.

Enzyme and pathway databases

UniPathwayiUPA00696.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls."
    Bauer S., Vasu P., Persson S., Mort A.J., Somerville C.R.
    Proc. Natl. Acad. Sci. U.S.A. 103:11417-11422(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  3. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
    Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
    , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
    Fungal Genet. Biol. 46:S2-13(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  4. "Comprehensive genomic analysis of cell wall genes in Aspergillus nidulans."
    de Groot P.W., Brandt B.W., Horiuchi H., Ram A.F., de Koster C.G., Klis F.M.
    Fungal Genet. Biol. 46:S72-S81(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiBTGE_EMENI
AccessioniPrimary (citable) accession number: Q5BD29
Secondary accession number(s): C8VMY9, Q1HFV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: April 26, 2005
Last modified: April 1, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.