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Protein

Kynureninase 2

Gene

bna5-2

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively.UniRule annotation

Catalytic activityi

L-kynurenine + H2O = anthranilate + L-alanine.UniRule annotation
L-3-hydroxykynurenine + H2O = 3-hydroxyanthranilate + L-alanine.UniRule annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotation

Pathwayi: L-kynurenine degradation

This protein is involved in step 1 of the subpathway that synthesizes L-alanine and anthranilate from L-kynurenine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Kynureninase 1 (bna5-1), Kynureninase 2 (bna5-2)
This subpathway is part of the pathway L-kynurenine degradation, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-alanine and anthranilate from L-kynurenine, the pathway L-kynurenine degradation and in Amino-acid degradation.

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes quinolinate from L-kynurenine.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Kynurenine 3-monooxygenase (bna4)
  2. Kynureninase 1 (bna5-1), Kynureninase 2 (bna5-2)
  3. 3-hydroxyanthranilate 3,4-dioxygenase (BNA1)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from L-kynurenine, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei144Pyridoxal phosphate; via amide nitrogenUniRule annotation1
Binding sitei145Pyridoxal phosphateUniRule annotation1
Binding sitei258Pyridoxal phosphateUniRule annotation1
Binding sitei261Pyridoxal phosphateUniRule annotation1
Binding sitei283Pyridoxal phosphateUniRule annotation1
Binding sitei323Pyridoxal phosphateUniRule annotation1
Binding sitei351Pyridoxal phosphateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00253; UER00329.
UPA00334; UER00455.

Names & Taxonomyi

Protein namesi
Recommended name:
Kynureninase 2UniRule annotation (EC:3.7.1.3UniRule annotation)
Alternative name(s):
Biosynthesis of nicotinic acid protein 5-2UniRule annotation
L-kynurenine hydrolase 2UniRule annotation
Gene namesi
Name:bna5-2
ORF Names:AN1857
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome VII
  • UP000005890 Componenti: Partially assembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003569781 – 474Kynureninase 2Add BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei284N6-(pyridoxal phosphate)lysineUniRule annotation1

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ5BC73.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni172 – 175Pyridoxal phosphate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the kynureninase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000242438.
InParanoidiQ5BC73.
KOiK01556.
OMAiGWYGGDK.
OrthoDBiEOG092C20ON.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01970. Kynureninase. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR010111. Kynureninase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR14084. PTHR14084. 1 hit.
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF038800. KYNU. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01814. kynureninase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5BC73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNHVNGVNG VNGVNGVKPV FPENAASKEY AKALDAADPL ASFRDKFIIP
60 70 80 90 100
SKANIQSKRL AKPNISADPC IYFCGNSLGL QPKATAKYME AHLDTWASIG
110 120 130 140 150
VNGHFTKIED SPLDPWWVMA EQAAGSMSKL VGAAPEEVIA MGTLTSNLHL
160 170 180 190 200
LLASFYKPTA TKHKILLDWK AFPSDHYAIE SHIAWHDGLD PKKSMVLIGP
210 220 230 240 250
DEGEYEIPTQ KILSIIDEHA DEAALILLPG IQYYTGQYFD INTITEYAHS
260 270 280 290 300
KGLMVGWDLA HAFANVELKL HEWDVDFAVW CTYKYANAGP GSMGGLFVHE
310 320 330 340 350
KHGKVDYSQG EDSPQFRHRL AGWYGGDRSV RFKMDNKFRP IPGAGGFQLS
360 370 380 390 400
TSSATDLTCL NASLSIFDQT SISELRRKSV QLTAYLEYLL LKDTTDETRP
410 420 430 440 450
FRIITPSNPE ERGAQLSLLL KPGLLQRVAD KLQSASIVCD KREPGVVRVA
460 470
PAPLYNTYSE VWQFVEQLKA AFQE
Length:474
Mass (Da):52,467
Last modified:November 16, 2011 - v2
Checksum:i12D43A644E6F499F
GO

Sequence cautioni

The sequence EAA65022 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACD01000029 Genomic DNA. Translation: EAA65022.1. Sequence problems.
BN001307 Genomic DNA. Translation: CBF85703.1.
RefSeqiXP_659461.1. XM_654369.1.

Genome annotation databases

EnsemblFungiiCADANIAT00008511; CADANIAP00008511; CADANIAG00008511.
EAA65022; EAA65022; AN1857.2.
GeneIDi2875742.
KEGGiani:AN1857.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACD01000029 Genomic DNA. Translation: EAA65022.1. Sequence problems.
BN001307 Genomic DNA. Translation: CBF85703.1.
RefSeqiXP_659461.1. XM_654369.1.

3D structure databases

ProteinModelPortaliQ5BC73.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANIAT00008511; CADANIAP00008511; CADANIAG00008511.
EAA65022; EAA65022; AN1857.2.
GeneIDi2875742.
KEGGiani:AN1857.2.

Phylogenomic databases

HOGENOMiHOG000242438.
InParanoidiQ5BC73.
KOiK01556.
OMAiGWYGGDK.
OrthoDBiEOG092C20ON.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00329.
UPA00334; UER00455.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPiMF_01970. Kynureninase. 1 hit.
InterProiIPR000192. Aminotrans_V_dom.
IPR010111. Kynureninase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR14084. PTHR14084. 1 hit.
PfamiPF00266. Aminotran_5. 1 hit.
[Graphical view]
PIRSFiPIRSF038800. KYNU. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01814. kynureninase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKYNU2_EMENI
AccessioniPrimary (citable) accession number: Q5BC73
Secondary accession number(s): C8VKF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: November 16, 2011
Last modified: November 30, 2016
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.