Reviewed, UniProtKB/Swiss-Prot Q5BB37 (CARA_EMENI)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase arginine-specific small chain
      Short name=CPS-A
    EC=6.3.5.5
Alternative name(s):
    Arginine-specific carbamoyl-phosphate synthetase, glutamine chain

Gene names

Name:	cpa-1
ORF Names:	AN2243

Organism

Emericella nidulans (Aspergillus nidulans) [Complete proteome]

Taxonomic identifier

162425 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Carbamoyl-phosphate synthase arginine-specific small chain		PRO_0000290595
Regions
	Domain	219 – 369	151	Glutamine amidotransferase type-1
	Compositional bias	406 – 417	12	Poly-Ala
Sites
	Active site	295	1	Nucleophile By similarity
	Active site	342	1	By similarity
	Active site	344	1	By similarity
Experimental info
	Sequence conflict	14	1	A → P in CAA11831. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q5BB37-1 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 8A72EF69232C40AB	FASTA	417	45,622
	10 20 30 40 50 60 MMFSRFFKAV PARAPAFSSP LPVYQARTMA TVRNQRPVER ATFTIRDGPI FHGKSFGART 70 80 90 100 110 120 TISGEAVFTT SLVGYPESLT DPSYRGQILV FTQPLIGNYG VPSTERDRHG LLKYFESPNL 130 140 150 160 170 180 QAAGVVVADV AEQYSHWTAV QSLGEWCARE GVPAISGVDT RAIVTYLREQ GSSLARITVG 190 200 210 220 230 240 EEYDADQDEA FTDPEQIHLV RQVSTKAPFH VSAADPQCHV AVLDCGVKEN ILRSLVSRGA 250 260 270 280 290 300 SITVFPFDYP IHKVAHHFDG VFISNGPGDP THCQDTTYHL RRLMETSQVP IFGICLGHQL 310 320 330 340 350 360 LALAADASTL PSDWKPYFVN LNDSSNEGMI HKSRPIFSTQ FHPEAKGGPL DSSYLFDIYI 370 380 390 400 410 DSVKKYKNSQ LAFHPSRETI PSPLLVDLLP KERVDVAPTI GMQNVAAAAA AAAAATA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed: 16372000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC A4 / M139.
[2]	"A CPA-like gene from Aspergillus nidulans." Doonan J. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-304.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACD01000036 Genomic DNA. Translation: EAA63928.1. AJ224085 mRNA. Translation: CAA11831.1.
RefSeq	XP_659847.1.
3D structure databases
HSSP	HSSP built from PDB template 1CE8 based on UniProtKB P0A6F1.
SMR	Q5BB37. Positions 39-369.
ModBase	Search...
Genome annotation databases
GeneID	2875485.
KEGG	ani:AN2243.2.
Phylogenomic databases
PhylomeDB	Q5BB37.
Enzyme and pathway databases
BRENDA	6.3.5.5. 3859.
Family and domain databases
InterPro	IPR001317. CarbamoylP_synth_GATase_dom. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_EMENI

Accession

Primary (citable) accession number: Q5BB37
Secondary accession number(s): O42806

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 12, 2007
Last sequence update:	April 26, 2005
Last modified:	February 9, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)