Reviewed,
UniProtKB/Swiss-Prot Q5BB37 (CARA_EMENI)
Last modified
February 9, 2010.
Version 34.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Carbamoyl-phosphate synthase arginine-specific small chain Short name=CPS-A EC=6.3.5.5 Alternative name(s): Arginine-specific carbamoyl-phosphate synthetase, glutamine chain | ||||
| Gene names |
| ||||
| Organism | Emericella nidulans (Aspergillus nidulans) [Complete proteome] | ||||
| Taxonomic identifier | 162425 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella |
Protein attributes
| Sequence length | 417 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Catalytic activity | 2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. |
| Pathway | Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. |
| Subunit structure | Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity. |
| Subcellular location | Cytoplasm By similarity. |
| Sequence similarities | Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Amino-acid biosynthesis Arginine biosynthesis |
| Cellular component | Cytoplasm |
| Domain | Glutamine amidotransferase |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Ligase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW carbamoyl-phosphate synthase (glutamine-hydrolyzing) activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 417 | 417 | Carbamoyl-phosphate synthase arginine-specific small chain | PRO_0000290595 | |||||
Regions | |||||||||
| Domain | 219 – 369 | 151 | Glutamine amidotransferase type-1 | ||||||
| Compositional bias | 406 – 417 | 12 | Poly-Ala | ||||||
Sites | |||||||||
| Active site | 295 | 1 | Nucleophile By similarity | ||||||
| Active site | 342 | 1 | By similarity | ||||||
| Active site | 344 | 1 | By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 14 | 1 | A → P in CAA11831. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. Birren B.W.Nature 438:1105-1115(2005) [PubMed: 16372000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC A4 / M139. |
| [2] | "A CPA-like gene from Aspergillus nidulans." Doonan J. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-304. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AACD01000036 Genomic DNA. Translation: EAA63928.1. AJ224085 mRNA. Translation: CAA11831.1. |
| RefSeq | XP_659847.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1CE8 based on UniProtKB P0A6F1. |
| SMR | Q5BB37. Positions 39-369. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 2875485. |
| KEGG | ani:AN2243.2. |
Phylogenomic databases | |
| PhylomeDB | Q5BB37. |
Enzyme and pathway databases | |
| BRENDA | 6.3.5.5. 3859. |
Family and domain databases | |
| InterPro | IPR001317. CarbamoylP_synth_GATase_dom. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view] |
| PANTHER | PTHR11405:SF4. CarA_synth_small. 1 hit. |
| Pfam | PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view] |
| PRINTS | PR00099. CPSGATASE. PR00096. GATASE. |
| TIGRFAMs | TIGR01368. CPSaseIIsmall. 1 hit. |
| PROSITE | PS51273. GATASE_TYPE_1. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | CARA_EMENI | ||||||||
| Accession | Primary (citable) accession number: Q5BB37 Secondary accession number(s): O42806 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | FPAP (Fungal Proteome Annotation Project) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


