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Protein

Endo-1,3(4)-beta-glucanase xgeA

Gene

xgeA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Mixed-linked glucanase involved in the degradation of complex natural cellulosic substrates. Active on laminarin. lichenan, soluble carboxymethyl cellulose but not on pustulan.1 Publication

Catalytic activityi

Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei142 – 1421NucleophileBy similarity
Active sitei147 – 1471Proton donorBy similarity

GO - Molecular functioni

  1. glucan endo-1,3-beta-glucanase activity, C-3 substituted reducing group Source: UniProtKB-EC
  2. glucan endo-1,4-beta-glucanase activity, C-3 substituted reducing group Source: UniProtKB-EC
  3. licheninase activity Source: UniProtKB

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
  2. glucan catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.
mycoCLAPiMLG16A_EMENI.

Names & Taxonomyi

Protein namesi
Recommended name:
Endo-1,3(4)-beta-glucanase xgeA (EC:3.2.1.6)
Alternative name(s):
Mixed-linked glucanase xgeA
Gene namesi
Name:xgeA
ORF Names:AN2385
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
ProteomesiUP000000560 Componenti: Chromosome VII

Subcellular locationi

  1. Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 603578Endo-1,3(4)-beta-glucanase xgeAPRO_0000395093Add
BLAST
Propeptidei604 – 62623Removed in mature formSequence AnalysisPRO_0000395094Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi61 – 611N-linked (GlcNAc...)Sequence Analysis
Lipidationi603 – 6031GPI-anchor amidated glycineSequence Analysis

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi342 – 459118Thr-richAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 16 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG12793.
HOGENOMiHOG000166269.
InParanoidiQ5BAP5.
OMAiSCVNYVA.
OrthoDBiEOG7HB5KN.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5BAP5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSLMRRVG SLAASAIIFP GIAHAASNYK LKESWEGEKI LNHFHFFDNA
60 70 80 90 100
DPTNGFVTYV NQSYAESAGL VKTTDSGSLY LGVDYENVLT VDGPGRESVR
110 120 130 140 150
IESNEYYDQG LYVVDIQHMP GSICGTWPAF WTVGPDWPTD GEIDIIEGVN
160 170 180 190 200
KHDANKIVLH TSDTCDVGGG YKMTGDMTSS ECGEASGTIG CVVQGKQGSS
210 220 230 240 250
GDPFNEQGGG VYAMEWQEKY LKIWYFPRSS IPESLTAGTP DVSSFGTPMA
260 270 280 290 300
HLQGSCNFKE RFTHQKLILD TTFCGDWAGG VFGDSGCPVS DPSDPMLSCK
310 320 330 340 350
NYVAENPAVY KNAYWELNSI KIYQLGGTAE VEGTQSAAAE STAAEATAAE
360 370 380 390 400
TTAAATQTAN GGSIEEITTS THSVTRTKTV SATHSTETAA VTETAAATTA
410 420 430 440 450
AASVASEVDA TNTQPVSKTK STSYVTSTTT LCPVESSQAA ATESVSRTKT
460 470 480 490 500
TSYVTITTTL CPVESLQTAN AVPSAKASTD AAAATTPAAE PHPSNAETPA
510 520 530 540 550
DSKSSADAVT AQATKTTIAV NTPNPATDSA SSVPPDSIVY TAPEVTSSSS
560 570 580 590 600
VPLFTIVSSS SQFVTVPTAA PSSFEPTDAV RDGADSYSTA ASPTTPSNPV
610 620
FTGVGSKVSI SASVAIAAFV MLLLVN
Length:626
Mass (Da):65,098
Last modified:April 26, 2005 - v1
Checksum:iB18C705CD41F4045
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACD01000039 Genomic DNA. Translation: EAA64496.1.
BN001307 Genomic DNA. Translation: CBF86736.1.
RefSeqiXP_659989.1. XM_654897.1.

Genome annotation databases

EnsemblFungiiCADANIAT00009087; CADANIAP00009087; CADANIAG00009087.
GeneIDi2874796.
KEGGiani:AN2385.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACD01000039 Genomic DNA. Translation: EAA64496.1.
BN001307 Genomic DNA. Translation: CBF86736.1.
RefSeqiXP_659989.1. XM_654897.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.
mycoCLAPiMLG16A_EMENI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANIAT00009087; CADANIAP00009087; CADANIAG00009087.
GeneIDi2874796.
KEGGiani:AN2385.2.

Phylogenomic databases

eggNOGiNOG12793.
HOGENOMiHOG000166269.
InParanoidiQ5BAP5.
OMAiSCVNYVA.
OrthoDBiEOG7HB5KN.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
[Graphical view]
SUPFAMiSSF49899. SSF49899. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  2. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
    Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
    , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
    Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  3. "Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls."
    Bauer S., Vasu P., Persson S., Mort A.J., Somerville C.R.
    Proc. Natl. Acad. Sci. U.S.A. 103:11417-11422(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Entry informationi

Entry nameiEGLX_EMENI
AccessioniPrimary (citable) accession number: Q5BAP5
Secondary accession number(s): C8VNP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: April 26, 2005
Last modified: April 1, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.