Q5BAB7 (BUD32_EMENI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase bud32
EC=2.7.11.1

Gene names

Name:	bud32
ORF Names:	AN2513

Organism

Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)

Taxonomic identifier

227321 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the EKC/KEOPS complex which promotes both telomere uncapping and telomere elongation By similarity. The complex is required for efficient recruitment of transcriptional coactivators. Important for bud site selection By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Component of the EKC/KEOPS complex By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Chromosome › telomere By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Chromosome Cytoplasm Nucleus Telomere
Ligand	ATP-binding Nucleotide-binding
Molecular function	Activator Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome, telomeric region Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Serine/threonine-protein kinase bud32		PRO_0000278914
Regions
	Domain	25 – 285	261	Protein kinase
	Nucleotide binding	31 – 39	9	ATP By similarity
Sites
	Active site	178	1	Proton acceptor By similarity
	Binding site	54	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5BAB7 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: D5D70F405B095F06	FASTA	285	31,806
	10 20 30 40 50 60 MPPTNRPPPF STILSSASLP SYSSTDELTP IYQGAEAHLY KTTFLSPSQP AALKIRPSKP 70 80 90 100 110 120 YRHPILDRRL TRARILQEAR CLQKLVKEGV SVPALLGVDW EPSAGDGSSW LVMEWIEGEP 130 140 150 160 170 180 VRVILEEWEA YLKGIEREKR LGLGEGVQGS EEEKVRGLMR RIGRAVGGLH RAGVIHGDLT 190 200 210 220 230 240 TSNLMLRPLG SADTTETIEE RDQSPSMAGE VVMIDFGLAM QSSQDEDRAV DLYVLERAFG 250 260 270 280 SSHPRTERFF EEVLVGYRES YKGAVSALKR LEDVRMRGRK RSMIG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed: 16372000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[2]	"The 2008 update of the Aspergillus nidulans genome annotation: a community effort." Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G. , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G. Fungal Genet. Biol. 46:S2-13(2009) [PubMed: 19146970] [Abstract] Cited for: GENOME REANNOTATION. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACD01000042 Genomic DNA. Translation: EAA63998.1. BN001307 Genomic DNA. Translation: CBF87005.1.
RefSeq	XP_660117.1. XM_655025.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5BAB7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADANIAT00009239; CADANIAP00009239; CADANIAG00009239.
GeneID	2874964.
KEGG	ani:AN2513.2.
Phylogenomic databases
OMA	MLLKPPL.
OrthoDB	EOG4R7ZKZ.
PhylomeDB	Q5BAB7.
Family and domain databases
InterPro	IPR022495. Kinase-assoc_endopept-1. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017442. Se/Thr_kinase-like_dom. IPR008266. Tyr_kinase_AS. [Graphical view]
KO	K08851.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
TIGRFAMs	TIGR03724. Arch_bud32. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BUD32_EMENI

Accession

Primary (citable) accession number: Q5BAB7
Secondary accession number(s): C8VPJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 6, 2007
Last sequence update:	April 26, 2005
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program