Reviewed, UniProtKB/Swiss-Prot Q5B8X0 (TRM10_EMENI)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    tRNA methyltransferase 10

Gene names

Name:	trm10
ORF Names:	AN3010

Organism

Emericella nidulans (Aspergillus nidulans) [Complete proteome]

Taxonomic identifier

162425 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in cytoplasmic tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the RNA methyltransferase trmD family. TRM10 subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	tRNA (guanine-N(1)-)-methyltransferase		PRO_0000060515

Sequences

	Sequence		Length	Mass (Da)
	Q5B8X0-1 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 6BAEC34EA09F58A2	FASTA	415	46,963
	10 20 30 40 50 60 MEEEERPRKI QKLGSSEAHE SEPLITGAIK GSQDDTSPAE QTNSQKAIPG ATTDNSKESE 70 80 90 100 110 120 EPASSTATDG PETTEQKISK RQLRRQAKLE QWEAKREERK IIRKQKTAAR KERRRELWEE 130 140 150 160 170 180 AKREGKDPNE ELNKLFPMTT RTRHRKSTRL PLTLIIDCGF DDLMQDKERV SLGQQLTRSY 190 200 210 220 230 240 SENNKSAFNG HLIISSFNKK LKERFETVLH KTHEGWKGVR FTGEDWLQAA KEASEVMQGP 250 260 270 280 290 300 NGGKLVGPFE DKTDAKPEDG EIVYLTSDSS ETLTELKPYS TYIIGGLVDK NRHKGICHKR 310 320 330 340 350 360 ATELGIRTAK LPIGQYIQMN SRPVLATNHV VEIMVRWLQL RDWAEAFMQT LPPRKGGALK 370 380 390 400 410 DSVKGQRDST PRDDVESNTE KGTVSETVED TEATEPAEAV TPKDIDAVQE NKQDE

References

[1]

"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed: 16372000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC 4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AACD01000051 Genomic DNA. Translation: EAA63581.1.
RefSeq	XP_660614.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2873735.
KEGG	ani:AN3010.2.
Enzyme and pathway databases
BRENDA	2.1.1.31. 3859.
Family and domain databases
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR007356. tRNA_m1G_MeTrfase_euk. [Graphical view]
PANTHER	PTHR13563. DUF425. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRM10_EMENI

Accession

Primary (citable) accession number: Q5B8X0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	April 26, 2005
Last modified:	September 22, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)