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Protein

Mannan endo-1,4-beta-mannosidase B

Gene

manB

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Endo-1,4-mannanase that catalyzes the random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans and heteromannans. It is a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans. Active against locust bean gum and gum guar (PubMed:16844780). Also has efficient transglycosylation activity. Produces mainly mannopentaose and mannoheptaose out of mannotetraose. May even prefer sugars as acceptors instead of water (PubMed:24950755).2 Publications

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.1 Publication

Kineticsi

  1. KM=0.6 mg/ml for locust bean gum mannan1 Publication

    pH dependencei

    Optimum pH is 6.1 Publication

    Temperature dependencei

    Optimum temperature is 60 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei204Proton donor/acceptorBy similarity1
    Binding sitei240SubstrateBy similarity1
    Binding sitei283SubstrateBy similarity1
    Active sitei312NucleophileBy similarity1

    GO - Molecular functioni

    • mannan endo-1,4-beta-mannosidase activity Source: UniProtKB

    GO - Biological processi

    • mannan catabolic process Source: UniProtKB

    Keywordsi

    Molecular functionGlycosidase, Hydrolase
    Biological processCarbohydrate metabolism

    Protein family/group databases

    CAZyiGH5. Glycoside Hydrolase Family 5.
    mycoCLAPiMAN5A_EMENI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannan endo-1,4-beta-mannosidase B (EC:3.2.1.781 Publication)
    Alternative name(s):
    Beta-mannanase 5B
    Short name:
    Man5B1 Publication
    Endo-beta-1,4-mannanase B
    Gene namesi
    Name:manB
    ORF Names:AN3297
    OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
    Taxonomic identifieri227321 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    Proteomesi
    • UP000000560 Componenti: Chromosome VI
    • UP000005890 Componenti: Partially assembled WGS sequence

    Subcellular locationi

    • Secreted By similarity

    GO - Cellular componenti

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 241 PublicationAdd BLAST24
    ChainiPRO_000039370825 – 387Mannan endo-1,4-beta-mannosidase BAdd BLAST363

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi107N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
    Glycosylationi165N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
    Disulfide bondi207 ↔ 210By similarity
    Glycosylationi286N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
    Glycosylationi318N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ5B833.
    SMRiQ5B833.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni204 – 206Substrate bindingBy similarity3

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000169951.
    InParanoidiQ5B833.
    KOiK19355.
    OrthoDBiEOG092C2KMO.

    Family and domain databases

    InterProiView protein in InterPro
    IPR001547. Glyco_hydro_5.
    IPR017853. Glycoside_hydrolase_SF.
    PfamiView protein in Pfam
    PF00150. Cellulase. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5B833-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRLIHAVLGL LAGAAPALVA ASPAAPIGNG RDQVSKAVGR HFEIDGKVQY
    60 70 80 90 100
    FAGTNCWWLG NLLNDFEVEL AVSQIAETGY KVVRTWGFFG VNDPSNPGQP
    110 120 130 140 150
    VYYQVLNESL YEGGLGINYG SNGIRRLDTV VSLAERYDIQ LVLTFMNNWN
    160 170 180 190 200
    DFGGINIYSN AFGSNATTWY TDKKSQRAYR EYIKFIVNRY KGSSAIFAWE
    210 220 230 240 250
    LGNEPRCKGC DPSVIYNWAK SVSAYIKKLD KKHMVALGDE GWLCPPEGDG
    260 270 280 290 300
    TYAYDCSEGV DFVKNLEIET LDYGTFHLYP ESWGYNYSWG SEWVLQHDAI
    310 320 330 340 350
    GKRFNKPVVF EEYGTPLNHT QLERPWQLTT VKETQVAADF IWQFGTVLPV
    360 370 380
    EGTEWGDVNS IYYGTEEYEV LAVQHAWEMA RKKVPRH
    Length:387
    Mass (Da):43,725
    Last modified:April 20, 2010 - v2
    Checksum:i8CC8A4C1253E7823
    GO

    Sequence cautioni

    The sequence CBF83007 differs from that shown. Reason: Erroneous gene model prediction.Curated
    The sequence EAA63265 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    DQ490485 mRNA. Translation: ABF50861.1.
    AACD01000055 Genomic DNA. Translation: EAA63265.1. Sequence problems.
    BN001306 Genomic DNA. Translation: CBF83007.1. Sequence problems.
    RefSeqiXP_660901.1. XM_655809.1.

    Genome annotation databases

    EnsemblFungiiCADANIAT00009761; CADANIAP00009761; CADANIAG00009761.
    EAA63265; EAA63265; AN3297.2.
    GeneIDi2873762.
    KEGGiani:AN3297.2.

    Similar proteinsi

    Entry informationi

    Entry nameiMANB_EMENI
    AccessioniPrimary (citable) accession number: Q5B833
    Secondary accession number(s): C8VI04, Q1HFT9
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 20, 2010
    Last sequence update: April 20, 2010
    Last modified: August 30, 2017
    This is version 70 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families