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Protein

Mannan endo-1,4-beta-mannosidase A

Gene

manA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Endo-1,4-mannanase that catalyzes the random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans and heteromannans. It is a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans. Active against locust bean gum and gum guar (PubMed:16844780). Also has transglycosylation activity (PubMed:21867780, PubMed:24950755).3 Publications

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.1 Publication

Kineticsi

  1. KM=1.4 mg/ml for locust bean gum mannan1 Publication
  2. KM=2.9 mM for mannopentaose1 Publication
  3. KM=1.8 mM for mannohexaose1 Publication

    pH dependencei

    Optimum pH is 5.5.2 Publications

    Temperature dependencei

    Optimum temperature is 52 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei211Proton donor/acceptorBy similarity1
    Binding sitei283SubstrateBy similarity1
    Active sitei312NucleophileBy similarity1

    GO - Molecular functioni

    • mannan endo-1,4-beta-mannosidase activity Source: UniProtKB

    GO - Biological processi

    • mannan catabolic process Source: UniProtKB

    Keywordsi

    Molecular functionGlycosidase, Hydrolase
    Biological processCarbohydrate metabolism

    Enzyme and pathway databases

    BRENDAi3.2.1.78. 517.

    Protein family/group databases

    CAZyiGH5. Glycoside Hydrolase Family 5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannan endo-1,4-beta-mannosidase A (EC:3.2.1.781 Publication)
    Alternative name(s):
    Endo-beta-1,4-mannanase A
    Gene namesi
    Name:manA
    Synonyms:man1
    ORF Names:AN3358
    OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
    Taxonomic identifieri227321 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
    Proteomesi
    • UP000000560 Componenti: Chromosome VI
    • UP000005890 Componenti: Unassembled WGS sequence

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 18Sequence analysisAdd BLAST18
    ChainiPRO_000039370619 – 383Mannan endo-1,4-beta-mannosidase AAdd BLAST365

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi75N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
    Glycosylationi199N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
    Disulfide bondi214 ↔ 217By similarity
    Disulfide bondi301 ↔ 308By similarity
    Disulfide bondi320 ↔ 369By similarity
    Glycosylationi332N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi162425.CADANIAP00009689.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5B7X2.
    SMRiQ5B7X2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni211 – 213Substrate bindingBy similarity3

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000169951.
    KOiK19355.
    OMAiWITAHVE.
    OrthoDBiEOG092C2KMO.

    Family and domain databases

    InterProiView protein in InterPro
    IPR001547. Glyco_hydro_5.
    IPR017853. Glycoside_hydrolase_SF.
    PfamiView protein in Pfam
    PF00150. Cellulase. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5B7X2-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKFSQALLSL ASLALAAALP HASTPVYTPS TTPSPTPTPS ASGSFATTSG
    60 70 80 90 100
    IQFVIDGEAG YFPGSNAYWI GFLKNNSDVD LVFDHMASSG LRILRVWGFN
    110 120 130 140 150
    DVNTAPTDGS VYFQLHQDGK STINTGKDGL QRLDYVVHSA EKHGIKLIIN
    160 170 180 190 200
    FVNYWDDYGG MNAYMRAYGG GDKADWFENE GIQAAYQAYV EAVVKRYINS
    210 220 230 240 250
    TAVFAWELAN EPRCTGCEPS VLHNWIEKTS AFIKGLDEKH LVCIGDGSDG
    260 270 280 290 300
    SYPFQYTEGS DFAAALTIDT IDFGTFHLYP DSWGTNNDWG KLWITSHAAA
    310 320 330 340 350
    CAAAGKPCLF EEYGVTSNHC AIEKQWQNAA LNATGIAADL YWQYGDTLSS
    360 370 380
    GPSPDDGNTF YYGSEEFECL VTNHVETIER SAK
    Length:383
    Mass (Da):41,789
    Last modified:April 26, 2005 - v1
    Checksum:i686126F8B2CF12C1
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    DQ490487 mRNA. Translation: ABF50863.1.
    AACD01000055 Genomic DNA. Translation: EAA63326.1.
    BN001306 Genomic DNA. Translation: CBF82875.1.
    RefSeqiXP_660962.1. XM_655870.1.

    Genome annotation databases

    EnsemblFungiiCADANIAT00009689; CADANIAP00009689; CADANIAG00009689.
    EAA63326; EAA63326; AN3358.2.
    GeneIDi2874351.
    KEGGiani:AN3358.2.

    Similar proteinsi

    Entry informationi

    Entry nameiMANA_EMENI
    AccessioniPrimary (citable) accession number: Q5B7X2
    Secondary accession number(s): C8VHS1, Q1HFT7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 20, 2010
    Last sequence update: April 26, 2005
    Last modified: October 25, 2017
    This is version 75 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families