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Protein

Pectinesterase A

Gene

pmeA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in maceration and soft-rotting of plant tissue. Active against citrus pectin.1 Publication

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

pH dependencei

Optimum pH is 8.0.1 Publication

Temperature dependencei

Optimum temperature is 30 degrees Celsius.1 Publication

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pectinesterase A (pmeA), Pectinesterase (AN7966.2), Pectinesterase (AN4860.2)
  2. Pectate lyase plyB (plyB)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei143 – 1431SubstrateBy similarity
Sitei165 – 1651Transition state stabilizerBy similarity
Active sitei166 – 1661Proton donorPROSITE-ProRule annotation
Active sitei187 – 1871NucleophilePROSITE-ProRule annotation
Binding sitei247 – 2471SubstrateBy similarity
Binding sitei249 – 2491SubstrateBy similarity

GO - Molecular functioni

  • aspartyl esterase activity Source: UniProtKB-KW
  • pectinesterase activity Source: UniProtKB

GO - Biological processi

  • cell wall modification Source: InterPro
  • pectin catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl esterase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823.

Protein family/group databases

mycoCLAPiPME8A_EMENI.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectinesterase A (EC:3.1.1.11)
Alternative name(s):
Pectin methylesterase A
Gene namesi
Name:pmeA
ORF Names:AN3390
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome VI
  • UP000005890 Componenti: Partially assembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:AN3390.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence analysisAdd
BLAST
Chaini19 – 325307Pectinesterase APRO_0000394083Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi126 – 1261N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ5B7U0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000217409.
InParanoidiQ5B7U0.
KOiK01051.
OMAiTINYAGA.
OrthoDBiEOG092C493C.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5B7U0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVQSYLSLF SLVGAALCAP REHFKRTART SAPAGCLTVG GSGTYSTIGA
60 70 80 90 100
AFAALGSSSS EACIYISAGT YKEQLTFQYA GPLTLYGETT DTSSYKKNTV
110 120 130 140 150
TITHTISSPE AGSLVASATV NAAMDNFTMY NINVVNGYGK GAQAVALAAS
160 170 180 190 200
GERQGYYGCQ FLGYQDTLYA RVGVQYYSNC YIEGAVDYIF GDASAWFGEC
210 220 230 240 250
DIVSNGAGYI TAMSRETASD PAWYCFDHCN IYGKSGLDLT GDVYLGRPWR
260 270 280 290 300
VLARVIYQNS ELSDIINAAG WTTMAEGATP LYYEIGNTGD GADTSKRLYL
310 320
SEISAAVTKA TVLGSDWTDW LDWSY
Length:325
Mass (Da):34,859
Last modified:April 26, 2005 - v1
Checksum:i3EA66689010AB75E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ490489 mRNA. Translation: ABF50865.1.
AACD01000055 Genomic DNA. Translation: EAA63358.1.
BN001306 Genomic DNA. Translation: CBF82804.1.
RefSeqiXP_660994.1. XM_655902.1.

Genome annotation databases

EnsemblFungiiCADANIAT00009650; CADANIAP00009650; CADANIAG00009650.
EAA63358; EAA63358; AN3390.2.
GeneIDi2874437.
KEGGiani:AN3390.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ490489 mRNA. Translation: ABF50865.1.
AACD01000055 Genomic DNA. Translation: EAA63358.1.
BN001306 Genomic DNA. Translation: CBF82804.1.
RefSeqiXP_660994.1. XM_655902.1.

3D structure databases

ProteinModelPortaliQ5B7U0.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

mycoCLAPiPME8A_EMENI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANIAT00009650; CADANIAP00009650; CADANIAG00009650.
EAA63358; EAA63358; AN3390.2.
GeneIDi2874437.
KEGGiani:AN3390.2.

Organism-specific databases

EuPathDBiFungiDB:AN3390.

Phylogenomic databases

HOGENOMiHOG000217409.
InParanoidiQ5B7U0.
KOiK01051.
OMAiTINYAGA.
OrthoDBiEOG092C493C.

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPMEA_EMENI
AccessioniPrimary (citable) accession number: Q5B7U0
Secondary accession number(s): C8VHN2, Q1HFT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: April 26, 2005
Last modified: September 7, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.