Q5B7R2 (CELB_EMENI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endo-beta-1,4-glucanase celB
Short name=Endoglucanase celB
EC=3.2.1.4

Alternative name(s):
Carboxymethylcellulase celB
Cellulase B

Gene names

Name:	celB
Synonyms:	eglB, eglC
ORF Names:	AN3418

Organism

Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) [Reference proteome]

Taxonomic identifier

227321 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella › Emericella nidulans › mitosporic Emericella nidulans

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has endoglucanase activity on substrates containing beta-1,4 glycosidic bonds, like in carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC) and beta-glucan. Involved in the degradation of complex natural cellulosic substrates. Ref.4
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 7 (cellulase C) family.
Biophysicochemical properties	pH dependence: Optimum pH is 5.5. Ref.4 Temperature dependence: Optimum temperature is 42 degrees Celsius.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW glucan catabolic process Inferred from direct assay Ref.4. Source: UniProtKB
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from direct assay Ref.4. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 430	414	Endo-beta-1,4-glucanase celB		PRO_0000395159
Sites
	Active site	216	1	Nucleophile By similarity
	Active site	221	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	43	1	N-linked (GlcNAc...) Potential
	Glycosylation	153	1	N-linked (GlcNAc...) Potential
	Glycosylation	395	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	45	1	S → Y in AAM54071. Ref.1
	Sequence conflict	73	1	P → R in AAM54071. Ref.1
	Sequence conflict	113	1	N → Y in AAM54071. Ref.1
	Sequence conflict	153	1	N → Y in AAM54071. Ref.1
	Sequence conflict	316	1	E → D in AAM54071. Ref.1
	Sequence conflict	345	1	C → W in AAM54071. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5B7R2 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 4807FA907F18749E	FASTA	430	45,929
	10 20 30 40 50 60 MALLLSLSLL ATTISAQQIG TPEIRPRLTT YHCTSANGCT EQNTSVVLDA ATHPIHDASN 70 80 90 100 110 120 PSVSCTTSNG LNPALCPDKQ TCADNCVIDG ITDYAAHGVE THGSRLTLTQ YRNVNGALSS 130 140 150 160 170 180 VSPRVYLVDE SDPDEQEYRA LSLLAQEFTF TVNVSALPCG MNGALYLSEM SPSGGRSALN 190 200 210 220 230 240 PAGASYGTGY CDAQCYVNPW INGEGNINGY GACCNEMDIW EANSRSTGFT PHACLYEPEE 250 260 270 280 290 300 TEGRGVYECA SEDECDSAGE NDGICDKWGC GFNPYALGNT EYYGRGQGFE VDTKEPFTVV 310 320 330 340 350 360 TQFLTDDGTS TGALTEIRRL YIQNGQVIEN AVVSSGADSL TDSLCASTAS WFDSYGGMEG 370 380 390 400 410 420 MGRALGRGMV LAMSIWNDAG GYMQWLDGGD AGPCNATEGA PEFIEEHTPW TRVVFEDLKW 430 GDIGSTFQAS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulation by carbon and nitrogen sources of a family of cellulases in Aspergillus nidulans." Lockington R.A., Rodbourn L., Barnett S., Carter C.J., Kelly J.M. Fungal Genet. Biol. 37:190-196(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[3]	"The 2008 update of the Aspergillus nidulans genome annotation: a community effort." Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G. , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G. Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[4]	"Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls." Bauer S., Vasu P., Persson S., Mort A.J., Somerville C.R. Proc. Natl. Acad. Sci. U.S.A. 103:11417-11422(2006) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF420021 Genomic DNA. Translation: AAM54071.1. AACD01000055 Genomic DNA. Translation: EAA63386.1. BN001306 Genomic DNA. Translation: CBF82749.1.
RefSeq	XP_661022.1. XM_655930.1.
3D structure databases
HSSP	HSSP built from PDB template 1EG1 based on UniProtKB P07981.
ProteinModelPortal	Q5B7R2.
ModBase	Search...
Protein family/group databases
CAZy	GH7. Glycoside Hydrolase Family 7.
mycoCLAP	EGL7A_EMENI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADANIAT00009621; CADANIAP00009621; CADANIAG00009621.
GeneID	2874128.
KEGG	ani:AN3418.2.
Phylogenomic databases
eggNOG	NOG138370.
HOGENOM	HOG000182210.
OMA	IWEANSR.
OrthoDB	EOG4XH37H.
Family and domain databases
Gene3D	2.70.100.10. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR001722. Glyco_hydro_7. [Graphical view]
Pfam	PF00840. Glyco_hydro_7. 1 hit. [Graphical view]
PRINTS	PR00734. GLHYDRLASE7.
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CELB_EMENI

Accession

Primary (citable) accession number: Q5B7R2
Secondary accession number(s): C8VHK3, Q8NK01

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	April 26, 2005
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program