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Protein

Endopolygalacturonase B

Gene

pgaB

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in maceration and soft-rotting of plant tissue. Hydrolyzes the 1,4-alpha glycosidic bonds of de-esterified pectate in the smooth region of the plant cell wall.1 Publication

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

pH dependencei

Optimum pH is 5.1.1 Publication

Temperature dependencei

Optimum temperature is 38 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei203 – 2031Proton donorPROSITE-ProRule annotation
Active sitei225 – 2251PROSITE-ProRule annotation

GO - Molecular functioni

  • polygalacturonase activity Source: UniProtKB

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • pectin catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Endopolygalacturonase B (EC:3.2.1.15)
Alternative name(s):
Pectinase B
Polygalacturonase B
Gene namesi
Name:pgaB
Synonyms:pecB
ORF Names:AN4372
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome III
  • UP000005890 Componenti: Partially assembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:AN4372.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Propeptidei21 – 299Sequence analysisPRO_0000393654
Chaini30 – 364335Endopolygalacturonase BPRO_0000393655Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi32 ↔ 47By similarity
Glycosylationi138 – 1381N-linked (GlcNAc...)Sequence analysis
Disulfide bondi205 ↔ 221By similarity
Disulfide bondi331 ↔ 336By similarity
Disulfide bondi355 ↔ 364By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Structurei

3D structure databases

ProteinModelPortaliQ5B508.
SMRiQ5B508. Positions 30-362.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati159 – 18830PbH1 1Add
BLAST
Repeati189 – 21022PbH1 2Add
BLAST
Repeati211 – 23121PbH1 3Add
BLAST
Repeati240 – 26122PbH1 4Add
BLAST
Repeati269 – 29123PbH1 5Add
BLAST
Repeati303 – 34846PbH1 6Add
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated
Contains 6 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000193107.
InParanoidiQ5B508.
KOiK01184.
OrthoDBiEOG092C33Y3.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 6 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5B508-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHFLQNSLIA AAMGAALVAA APAADLDARS SCTFTSASAA KSGASKCSTV
60 70 80 90 100
TLKSIQVPAG ETLDLTGLKS GATVIFEGET TFGYKEWKGP LISMSGDKIT
110 120 130 140 150
VKQASGAKIN CDGARWWDTK GSNGGKTKPK FFSAHKLNNS KIQGLKIYNT
160 170 180 190 200
PVQGFSIQSD HLTISDVTID NSAGTSKGHN TDAFDIGSST YITIDGATVY
210 220 230 240 250
NQDDCIAINS GEHITFTNGY CSGGHGLSIG SVGGRSDNTV KSVTISNSKV
260 270 280 290 300
VDSQNGVRIK TVYKATGSVT DVTFQDIELS GITKYGLIVE QDYENGSPTG
310 320 330 340 350
TPTNGVEVED ITFKKITGSV DSSATRVNIL CGSGSCKDWT WSGVDITGGK
360
KSSKCKNVPS GASC
Length:364
Mass (Da):37,857
Last modified:April 20, 2010 - v2
Checksum:iDB54DC4FFEE92897
GO

Sequence cautioni

The sequence CBF77661 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence EAA60289 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ490492 mRNA. Translation: ABF50868.1.
AACD01000076 Genomic DNA. Translation: EAA60289.1. Sequence problems.
BN001303 Genomic DNA. Translation: CBF77661.1. Sequence problems.
RefSeqiXP_661976.1. XM_656884.1.

Genome annotation databases

EnsemblFungiiCADANIAT00006086; CADANIAP00006086; CADANIAG00006086.
EAA60289; EAA60289; AN4372.2.
GeneIDi2872172.
KEGGiani:AN4372.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ490492 mRNA. Translation: ABF50868.1.
AACD01000076 Genomic DNA. Translation: EAA60289.1. Sequence problems.
BN001303 Genomic DNA. Translation: CBF77661.1. Sequence problems.
RefSeqiXP_661976.1. XM_656884.1.

3D structure databases

ProteinModelPortaliQ5B508.
SMRiQ5B508. Positions 30-362.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANIAT00006086; CADANIAP00006086; CADANIAG00006086.
EAA60289; EAA60289; AN4372.2.
GeneIDi2872172.
KEGGiani:AN4372.2.

Organism-specific databases

EuPathDBiFungiDB:AN4372.

Phylogenomic databases

HOGENOMiHOG000193107.
InParanoidiQ5B508.
KOiK01184.
OrthoDBiEOG092C33Y3.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 6 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGLRB_EMENI
AccessioniPrimary (citable) accession number: Q5B508
Secondary accession number(s): C8V8X1, Q1HFT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: April 20, 2010
Last modified: September 7, 2016
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.