Reviewed, UniProtKB/Swiss-Prot Q5B4C9 (ATG26_EMENI)

Names and origin

Protein names

Recommended name:
    Sterol 3-beta-glucosyltransferase
    EC=2.4.1.173
Alternative name(s):
    Autophagy-related protein 26

Gene names

Name:	atg26
ORF Names:	AN4601

Organism

Emericella nidulans (Aspergillus nidulans) [Complete proteome]

Taxonomic identifier

162425 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella

Protein attributes

Sequence length	1396 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in decane metabolism and autophagy. Involved in the biosynthesis of sterol glucoside By similarity.
Catalytic activity	UDP-glucose + a sterol = UDP + a sterol 3-beta-D-glucoside.
Subcellular location	Membrane; Peripheral membrane protein Potential.
Sequence similarities	Belongs to the glycosyltransferase 28 family. Contains 2 GRAM domains. Contains 1 PH domain.

Ontologies

Keywords
Biological process	Autophagy Lipid synthesis Protein transport Steroid biosynthesis Sterol biosynthesis Transport
Cellular component	Membrane
Domain	Repeat
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro lipid glycosylation Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sterol 3-beta-glucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1396	1396	Sterol 3-beta-glucosyltransferase		PRO_0000215610
Regions
	Domain	238 – 273	36	GRAM 1
	Domain	289 – 388	100	PH
	Domain	717 – 783	67	GRAM 2

Sequences

	Sequence		Length	Mass (Da)
	Q5B4C9-1 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: C6C05DFB6B278F47	FASTA	1,396	157,238
	10 20 30 40 50 60 MRPFIDDAKR RAERRLSASR QSISARRMFA SSFPDRLKVD ADDAQMDYTA PPSSNDSRDG 70 80 90 100 110 120 MQYMQQSVMS LIAAVGSRTD LRARFDDSSD SEEETRTRPR FLSEKPFDQQ ITASPRADVT 130 140 150 160 170 180 LHVPSAPETR SSSRERGRRH RRSISEHKLF RPFKAGSASQ DKKQDASPDP GPSTSDRLSP 190 200 210 220 230 240 IPVLPRPRSA TPRAAPILSR MVEARALLET ENPEENPQTL EEKEQGVSKK SQVSPLSRQL 250 260 270 280 290 300 MEMFRFPTPE KVVVEYACSL LQSMLLQGYM YVTEGHICFY AYLPRQSTRV IKSGYIYKRG 310 320 330 340 350 360 RKNPKYNRYW FSLKEDVLSY YADPSNLYFP SGQIDLRYGI SASLTEPKDK SRESRDFQVT 370 380 390 400 410 420 TDHRTYYFRA DSSVNAKEWV RALQKVIFRT HNEGESIKVA FPIENILDVE ESPMVEFAQT 430 440 450 460 470 480 FKIRVVESEE TYAIDEHYFT FFDPDSGRRA FDLLKGLISG TPTRSPPGLS PLPDHTAQPR 490 500 510 520 530 540 RSRGSQNRWS LTSGSKSQVV STRRQRSAST SILGSGTGNE NSPQRQEDSS SSFFNSIDQV 550 560 570 580 590 600 TESSAVLQSI TDTTESASQI LNRSDVFQSP TIHTWQQRTS GTARSNRRHS DEITRSTTEH 610 620 630 640 650 660 GLDGISLARD GPEMQYTNSD SEQESKDASR LYSAVALNEI VKAGTYPLQR AAGLAGYLKS 670 680 690 700 710 720 RSKEMSNLLA TESMGYIEKV SGMWAGGRRH YGETDGLLPD DRALYPETAE ESLRDRERFR 730 740 750 760 770 780 AHFALPPTEK LEAAYFAYLH RALPLYGKIY ISQNRLCFRS LLPGTRTKMI LPLHDIENVE 790 800 810 820 830 840 KEKGFQFGYH GLVVVIRGHE ELFFEFNAAD ARDDCAVTLH QRLESAKFLV ESISLSQQET 850 860 870 880 890 900 DESEAAKVEH RMLQEARRNA SAEQDLRPGL SESSELHSIF DDPRASIVNF KPAEPLKITC 910 920 930 940 950 960 LTIGSRGDVQ PYIALCKGLL AEGHKPKIAT HAEFEPWVRK HGIDFAPVDG DPAELMRLCV 970 980 990 1000 1010 1020 ENGMFTYSFL KEATAKFRGW IDDLLSSAWR ACQDSDLLIE SPSAMAGIHI AEALRIPYFR 1030 1040 1050 1060 1070 1080 GFTMPWSRTR AYPHAFAVPE SRLGGAYNYI TYVMFENVFW RAIAGQVNRW RMKELGLRAT 1090 1100 1110 1120 1130 1140 NLDKMQPNKV PFLYNFSPSV VPPPLDFPDW VRITGYWFLS ESSDWTPPRA LAEFIQCARQ 1150 1160 1170 1180 1190 1200 DGKKIVYIGF GSIVVSDPSA LTRTVVESVQ KADVRCILSK GWSARLGDPT STKVEIPLPP 1210 1220 1230 1240 1250 1260 EIHQIQSAPH DWLFSQIDAA AHHGGAGTTG ASLRAGVPTI IKPFFGDQFF FGNRVEDLGV 1270 1280 1290 1300 1310 1320 GICMKKLNVS VFSRALWTAT HDERMIVRAK QLGERIRSED GVATAIQAIY RDLEYATTLT 1330 1340 1350 1360 1370 1380 RQRSSISSTP FSPTPSTKTS DDQNANDDIA DIEDWTFIGD ESDLEFPRRA RERAVSGADN 1390 IPERTILGSR SIYADS

References

[1]

"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed: 16372000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC 4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AACD01000079 Genomic DNA. Translation: EAA60403.1.
RefSeq	XP_662205.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2872399.
KEGG	ani:AN4601.2.
Enzyme and pathway databases
BRENDA	2.4.1.173. 3859.
Family and domain databases
InterPro	IPR004276. Glyco_trans_28. IPR004182. GRAM. IPR011993. PH_type. IPR001849. Pleckstrin_homology. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF03033. Glyco_transf_28. 1 hit. PF02893. GRAM. 1 hit. PF00169. PH. 1 hit. [Graphical view]
SMART	SM00568. GRAM. 2 hits. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG26_EMENI

Accession

Primary (citable) accession number: Q5B4C9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	April 26, 2005
Last modified:	October 13, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)