Reviewed, UniProtKB/Swiss-Prot Q5B2N0 (KMO_EMENI)

Names and origin

Protein names

Recommended name:
    Kynurenine 3-monooxygenase
    EC=1.14.13.9
Alternative name(s):
    Kynurenine 3-hydroxylase
    Biosynthesis of nicotinic acid protein 4

Gene names

Name:	bna4
ORF Names:	AN5200

Organism

Emericella nidulans (Aspergillus nidulans) [Complete proteome]

Taxonomic identifier

162425 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid By similarity.
Catalytic activity	L-kynurenine + NADPH + O2 = 3-hydroxy-L-kynurenine + NADP+ + H2O.
Cofactor	FAD By similarity.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the aromatic-ring hydroxylase family. KMO subfamily.
Sequence caution	The sequence EAA62381.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Mitochondrion
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kynurenine 3-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Kynurenine 3-monooxygenase		PRO_0000361928

Sequences

	Sequence		Length	Mass (Da)
	Q5B2N0-1 [UniParc]. Last modified February 10, 2009. Version 2. Checksum: 28D6D202B34D4210	FASTA	506	56,440
	10 20 30 40 50 60 MASPAKQKVV IVGAGPVGCL AALYAAARGD DVELYELRGD LRVPGTIPLN FTKSINLSLS 70 80 90 100 110 120 HRGITALRHS GREHVINEIL QEVVPIYGRM IHGRDDGKLW EAPQAYDVHG RNNYSADRGM 130 140 150 160 170 180 LNNVFLNELE RIPNIKLFFN HKLTGADFQA NKAWFERRLP GEAPLPGSSG RVPEIEVDFD 190 200 210 220 230 240 FLIGADGAHS ATRYHMMKFA RVDYQQEYID TLWCEFRIPP SPTNDFLISP SHLHIWPGKE 250 260 270 280 290 300 FMFIALPSVD KSFTCTLFAP ASHYAQLERS TEDLLQFFDE HFPGVCPQLI SPSDLTAQFR 310 320 330 340 350 360 ANPHLPLISI KCAPHHYSSS VVIVGDAAHA VLPFYGQGLN AGLEDIQVLF DALDKHGVYN 370 380 390 400 410 420 ANSDQAARAL ARQSAFAAYT ASRTADAHAI NDLSRQNYVE MRWGVKQPLY RLRKYIEEAL 430 440 450 460 470 480 YHYLPSLGWQ TQYTRVSFSN QRYSEIIAIN RRQGRILGAV FGSTLISVLA VTGIYLWRQP 490 500 TTRLLSLASF RGALQGALQG ALTGTA

References

[1]

"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed: 16372000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC 4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AACD01000089 Genomic DNA. Translation: EAA62381.1. Sequence problems.
RefSeq	XP_662804.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2871494.
KEGG	ani:AN5200.2.
Family and domain databases
InterPro	IPR003042. Rng_hydrolase-like. [Graphical view]
PRINTS	PR00420. RNGMNOXGNASE.
ProtoNet	Search...

Entry information

Entry name

KMO_EMENI

Accession

Primary (citable) accession number: Q5B2N0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	February 10, 2009
Last modified:	September 22, 2009
This is version 38 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)