Reviewed, UniProtKB/Swiss-Prot Q5B0L1 (ATG15_EMENI)

Names and origin

Protein names

Recommended name:
    Putative lipase atg15
    EC=3.1.1.3
Alternative name(s):
    Autophagy-related protein 15

Gene names

Name:	atg15
ORF Names:	AN5919

Organism

Emericella nidulans (Aspergillus nidulans) [Complete proteome]

Taxonomic identifier

162425 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endosome Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	Putative lipase atg15		PRO_0000090368
Regions
	Topological domain	1 – 7	7	Cytoplasmic Potential
	Transmembrane	8 – 28	21	Signal-anchor for type II membrane protein Potential
	Topological domain	29 – 590	562	Lumenal Potential
	Compositional bias	459 – 563	105	Thr-rich
Sites
	Active site	308	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	153	1	N-linked (GlcNAc...) Potential
	Glycosylation	188	1	N-linked (GlcNAc...) Potential
	Glycosylation	210	1	N-linked (GlcNAc...) Potential
	Glycosylation	268	1	N-linked (GlcNAc...) Potential
	Glycosylation	292	1	N-linked (GlcNAc...) Potential
	Glycosylation	454	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5B0L1-1 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 43028660D0C23C57	FASTA	590	64,060
	10 20 30 40 50 60 MDLSVSTLLM SLALVLPSCV SAYQPVYFRS QEATPFIPPQ VPSADPQSLS GTHEFTLRHI 70 80 90 100 110 120 VQRGAYEYPE LHRRLDIKPN TQLRTVSEDG IEDNEPAVFN VPFVASSEPV TIERLADRRL 130 140 150 160 170 180 SVIEEHLAAA RSAGSAVTLS SSQWTKDTLA GPNVTDKKTI LTFAKMTAND YIEVPGTEDW 190 200 210 220 230 240 QDINGKLNYS TSFGWQNDGL RGHIYADDTN STIVISLKGT SPALFDGAGT TTNDKVNDNL 250 260 270 280 290 300 YFSCCCGQGG SYLWREVCDC QKSAFNANLT CIIEAMNDEN RYYRASLDLY SNVTELYPNA 310 320 330 340 350 360 NVWLTGHSLG GAMASLLGLT FGLPVVTFEA VPEALPAARL GLPTPPGYNP ALPQARKFTG 370 380 390 400 410 420 AYHFGHTADP VYMGTCNGVS SVCTWGGYAM ESACHTGQMC VYDTVADKGW RVAIGTHRIK 430 440 450 460 470 480 AVISDVIEVY DDLPQCAPEE ECYDCELWKF FRSNGSEITT TSSTTTTSTI TTSTRTTTCK 490 500 510 520 530 540 TPGWWGCLDD STTTDPPTTT TTTSSTCKTP GWFGCKDPTS TTATTTTEAP TTTTTCKDPG 550 560 570 580 590 WFGCRDPTSP TTSTAPQTST CETPGFFWGC YDTQTAVDHL ITPAPILIDL

References

[1]

"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed: 16372000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC A4 / M139.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACD01000101 Genomic DNA. Translation: EAA57782.1.
RefSeq	XP_663523.1.
3D structure databases
SMR	Q5B0L1. Positions 154-321.
ModBase	Search...
Genome annotation databases
GeneID	2870991.
KEGG	ani:AN5919.2.
Phylogenomic databases
PhylomeDB	Q5B0L1.
Enzyme and pathway databases
BRENDA	3.1.1.3. 3859.
Family and domain databases
InterPro	IPR002921. Lipase_3. [Graphical view]
Pfam	PF01764. Lipase_3. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_EMENI

Accession

Primary (citable) accession number: Q5B0L1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	April 26, 2005
Last modified:	January 19, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)