Q5AYH8 (BGLE_EMENI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable beta-glucosidase E
EC=3.2.1.21

Alternative name(s):
Beta-D-glucoside glucohydrolase E
Cellobiase E
Gentiobiase E

Gene names

Name:	bglE
ORF Names:	AN6652

Organism

Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) [Reference proteome]

Taxonomic identifier

227321 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella › Emericella nidulans › mitosporic Emericella nidulans

Protein attributes

Sequence length	1023 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Beta-glucosidases are one of a number of cellulolytic enzymes involved in the degradation of cellulosic biomass. Catalyzes the last step releasing glucose from the inhibitory cellobiose By similarity.
Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.
Pathway	Glycan metabolism; cellulose degradation.
Subcellular location	Cell membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 3 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Cell membrane Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1023	1023	Probable beta-glucosidase E		PRO_0000394874
Regions
	Topological domain	1 – 128	128	Cytoplasmic Potential
	Transmembrane	129 – 149	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	150 – 1023	874	Extracellular Potential
Sites
	Active site	415	1	By similarity
Amino acid modifications
	Glycosylation	199	1	N-linked (GlcNAc...) Potential
	Glycosylation	387	1	N-linked (GlcNAc...) Potential
	Glycosylation	458	1	N-linked (GlcNAc...) Potential
	Glycosylation	497	1	N-linked (GlcNAc...) Potential
	Glycosylation	848	1	N-linked (GlcNAc...) Potential
	Glycosylation	964	1	N-linked (GlcNAc...) Potential
	Glycosylation	979	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5AYH8 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: F69D09D353F85C37	FASTA	1,023	112,164
	10 20 30 40 50 60 MPKSYTPVHD SIPEEDHFSS DDESNFRLHR IDRSASRSQS PKENEGEPSI LAPLVRKSTD 70 80 90 100 110 120 FETYLDSLTE DEQQLLSASK DHDIEDLDRF GDGTAAARRR FSESKKRRKL LAKRGGWRAV 130 140 150 160 170 180 YYSKTWWRTL VVVIIALGLL VWGFLKYAST RGDIWEEYDM PGPDSYFPTP KGGTLKHWAE 190 200 210 220 230 240 SYEKASKLVE RMTLIEKVNI TTGTGWQMGI NSKLTISGPA ALVGFPSLCL QDGPLGIRFA 250 260 270 280 290 300 DHITAFPAGI TTGATWNRDL MRQRGAAIGL EARLKGVNVI LGPSMGPLGM MPAGGRNWEG 310 320 330 340 350 360 FGSDPVLQAV AAVETIHGIQ SNGVMATAKH YIMNEQEHFR QPNEWGIPYA LSSNIDDRAL 370 380 390 400 410 420 HEVFLWPFAE SIRADVASVM CSYNQVNNSH ACENSKLLNG ILKDELGFQG FVQSDWLAQR 430 440 450 460 470 480 SGVNSALGGL DMSMPGDGLH WADGRSLWGS ELTRAALNTS VPMERLNDMV TRIVAAWYQL 490 500 510 520 530 540 GQDSWESPAP DGDGGPNFSS WTDDEFGFRY PGSPGDTSAA RVNRFIDAQG RGEEGHWNIA 550 560 570 580 590 600 RKVAAEGIVL VKNVGGVLPL SRSPRANAER PYRVGVYGDD GGPAAGPNIC TDRGCNSGTL 610 620 630 640 650 660 AMGWGSGTVE FPYLISPIDA LQGAWQSDVQ MTPYLRNAVM PADTSDKDLC LVFVNADSGE 670 680 690 700 710 720 GYISAGGIHG DRNNLFLQKG GDTLVHTVAT NCGGPTVVVV HAVGPVIVEP WIDLPGVQAV 730 740 750 760 770 780 LFAHLPGEES GNALLDVLFG DVDASGRLPY TVGKSLEDYG PGAQVLYEPN APVPQVDFSD 790 800 810 820 830 840 ALYIDHRYFD RNNINPRYEF GFGLSYTKWE LTNMKITRLQ RNPSRLPAAR PPDAVAPPSY 850 860 870 880 890 900 DANPPLANES VLFPPGFRIL SKYIYPYLPT LEATTPPPPN PEASGSATDQ KPHRTKPSDA 910 920 930 940 950 960 GGGAGGNPSL YEEVARIDLT VQNTGTRSGQ QVIQLYVSFP HTVTESSGQK SHENIDFPDR 970 980 990 1000 1010 1020 VLRNFTKISL APGQKMDVNM TLTRKDLSYW SVREQNWVLP KDEFYFWVGY SSRNLPLGKP FDP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[2]	"The 2008 update of the Aspergillus nidulans genome annotation: a community effort." Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G. , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G. Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACD01000110 Genomic DNA. Translation: EAA58181.1. BN001301 Genomic DNA. Translation: CBF71178.1.
RefSeq	XP_664256.1. XM_659164.1.
3D structure databases
ProteinModelPortal	Q5AYH8.
ModBase	Search...
Protein family/group databases
CAZy	GH3. Glycoside Hydrolase Family 3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	CADANIAT00007435; CADANIAP00007435; CADANIAG00007435.
GeneID	2870419.
KEGG	ani:AN6652.2.
Phylogenomic databases
eggNOG	COG1472.
HOGENOM	HOG000031215.
KO	K05349.
OMA	GVMATAK.
OrthoDB	EOG4SFDF3.
Enzyme and pathway databases
UniPathway	UPA00696.
Family and domain databases
Gene3D	3.20.20.300. 1 hit. 3.40.50.1700. 1 hit.
InterPro	IPR026891. Fn3-like. IPR026892. Glyco_hydro_3. IPR002772. Glyco_hydro_3_C. IPR001764. Glyco_hydro_3_N. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR30620. PTHR30620. 1 hit.
Pfam	PF14310. Fn3-like. 1 hit. PF00933. Glyco_hydro_3. 1 hit. PF01915. Glyco_hydro_3_C. 1 hit. [Graphical view]
PRINTS	PR00133. GLHYDRLASE3.
SUPFAM	SSF52279. Glyco_hydro_3_C. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00775. GLYCOSYL_HYDROL_F3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGLE_EMENI

Accession

Primary (citable) accession number: Q5AYH8
Secondary accession number(s): C8V1D9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	April 26, 2005
Last modified:	March 6, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program