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Protein

Rhamnogalacturonate lyase A

Gene

rglA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Pectinolytic enzymes consist of four classes of enzymes: pectin lyase, polygalacturonase, pectin methylesterase and rhamnogalacturonase. Degrades the rhamnogalacturonan I (RG-I) backbone of pectin. Active against linseed rhamnogalacturonan.1 Publication

Catalytic activityi

Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end.

GO - Molecular functioni

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • pectin catabolic process Source: UniProtKB

Keywordsi

Molecular functionLyase
Biological processCarbohydrate metabolism, Cell wall biogenesis/degradation, Polysaccharide degradation

Protein family/group databases

CAZyiPL4 Polysaccharide Lyase Family 4
mycoCLAPiRGL4A_EMENI

Names & Taxonomyi

Protein namesi
Recommended name:
Rhamnogalacturonate lyase A (EC:4.2.2.23)
Gene namesi
Name:rglA
ORF Names:AN7135
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome IV
  • UP000005890 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000039437121 – 527Rhamnogalacturonate lyase AAdd BLAST507

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi27N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi46N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi50 ↔ 93By similarity
Disulfide bondi184 ↔ 193By similarity
Glycosylationi351N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ5AX45

Structurei

3D structure databases

ProteinModelPortaliQ5AX45
SMRiQ5AX45
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the polysaccharide lyase 4 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000171008
InParanoidiQ5AX45
KOiK18195
OMAiQPTGFRN
OrthoDBiEOG092C31LY

Family and domain databases

CDDicd10316 RGL4_M, 1 hit
Gene3Di2.60.120.260, 1 hit
2.70.98.10, 1 hit
InterProiView protein in InterPro
IPR013784 Carb-bd-like_fold
IPR011013 Gal_mutarotase_sf_dom
IPR008979 Galactose-bd-like_sf
IPR014718 GH-type_carb-bd
IPR029413 RG-lyase_II
IPR029411 RG-lyase_III
IPR016590 Rhamnogalacturonase_B
IPR015364 RhgB_N
PANTHERiPTHR36574 PTHR36574, 1 hit
PfamiView protein in Pfam
PF14683 CBM-like, 1 hit
PF14686 fn3_3, 1 hit
PF09284 RhgB_N, 1 hit
PIRSFiPIRSF011794 Rhamnogalacturonase_B, 1 hit
SUPFAMiSSF49452 SSF49452, 1 hit
SSF49785 SSF49785, 1 hit
SSF74650 SSF74650, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5AX45-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSKTFLLSS AVLWARVANA AFGITDNGSS YTIDANSPNP LKFTVNKSSC
60 70 80 90 100
DITSIVYYGS EFQYSGKGSH IGSGLGSATV SATQSGDYIK VTCDTSSLTH
110 120 130 140 150
YFVVHNGDPI IHMATYITAE PDIGELRFIA RLNSNLLPNE EPFGDVSTTS
160 170 180 190 200
GGSAIEGSDV FLVNGETRSK FYSSERFIDD HRHCISGSAH RVCMILNQYE
210 220 230 240 250
SSSGGPFFRD INSNNGGDYN ALYWYMNSGH VQTESFRTGL HGPYSMYFSR
260 270 280 290 300
SGTPSTNIDT SFFASLGIKG YVAANGRGTV TGKASGADSS MDWVVHWYNN
310 320 330 340 350
DAQYWTYTAS DGSFTSPAMK PGTYTMKYYQ GEFPVAETTV TVSAGSSTTK
360 370 380 390 400
NISGSVKTGT TIFKIGEWDG QPTGFRNADK QLRMHPSDSR MDSWSSTYTV
410 420 430 440 450
GSSSLSDFPM AVFKSVNNPV TIKFTATSAQ TGAATLRIGT TLSFAGGRPQ
460 470 480 490 500
ATINSYTGPA PSAPTNLNSR GVTRGAYRGL GEVYDVSVPA GTIVTGENTI
510 520
TISVISGSSG DAFLSPNVVF DCIELFQ
Length:527
Mass (Da):56,381
Last modified:April 26, 2005 - v1
Checksum:iD07A2C92955DB47D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ490504 mRNA Translation: ABF50880.1
AACD01000122 Genomic DNA Translation: EAA61387.1
BN001304 Genomic DNA Translation: CBF79004.1
RefSeqiXP_664739.1, XM_659647.1

Genome annotation databases

EnsemblFungiiCADANIAT00000323; CADANIAP00000323; CADANIAG00000323
EAA61387; EAA61387; AN7135.2
GeneIDi2870208
KEGGiani:AN7135.2

Similar proteinsi

Entry informationi

Entry nameiRGLA_EMENI
AccessioniPrimary (citable) accession number: Q5AX45
Secondary accession number(s): C8VD91, Q1HFS0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: April 26, 2005
Last modified: May 23, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health