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Protein

Rhamnogalacturonate lyase A

Gene

rglA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Pectinolytic enzymes consist of four classes of enzymes: pectin lyase, polygalacturonase, pectin methylesterase and rhamnogalacturonase. Degrades the rhamnogalacturonan I (RG-I) backbone of pectin. Active against linseed rhamnogalacturonan.1 Publication

Catalytic activityi

Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-(1->4)-alpha-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end.

GO - Molecular functioni

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • pectin catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Polysaccharide degradation

Protein family/group databases

CAZyiPL4. Polysaccharide Lyase Family 4.
mycoCLAPiRGL4A_EMENI.

Names & Taxonomyi

Protein namesi
Recommended name:
Rhamnogalacturonate lyase A (EC:4.2.2.23)
Gene namesi
Name:rglA
ORF Names:AN7135
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome IV
  • UP000005890 Componenti: Partially assembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:AN7135.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 527507Rhamnogalacturonate lyase APRO_0000394371Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi27 – 271N-linked (GlcNAc...)Sequence analysis
Glycosylationi46 – 461N-linked (GlcNAc...)Sequence analysis
Disulfide bondi50 ↔ 93By similarity
Disulfide bondi184 ↔ 193By similarity
Glycosylationi351 – 3511N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ5AX45.
SMRiQ5AX45. Positions 21-527.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the polysaccharide lyase 4 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000171008.
InParanoidiQ5AX45.
KOiK18195.
OMAiQPTGFRN.
OrthoDBiEOG092C31LY.

Family and domain databases

CDDicd10316. RGL4_M. 1 hit.
Gene3Di2.60.120.260. 1 hit.
2.60.40.1120. 1 hit.
2.70.98.10. 1 hit.
InterProiIPR013784. Carb-bd-like_fold.
IPR014766. CarboxyPept_regulatory_dom.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR029413. RG-lyase_II.
IPR029411. RG-lyase_III.
IPR016590. Rhamnogalacturonase_B.
IPR015364. RhgB_N.
[Graphical view]
PfamiPF14683. CBM-like. 1 hit.
PF14686. fn3_3. 1 hit.
PF09284. RhgB_N. 1 hit.
[Graphical view]
PIRSFiPIRSF011794. Rhamnogalacturonase_B. 1 hit.
SUPFAMiSSF49452. SSF49452. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF74650. SSF74650. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5AX45-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSKTFLLSS AVLWARVANA AFGITDNGSS YTIDANSPNP LKFTVNKSSC
60 70 80 90 100
DITSIVYYGS EFQYSGKGSH IGSGLGSATV SATQSGDYIK VTCDTSSLTH
110 120 130 140 150
YFVVHNGDPI IHMATYITAE PDIGELRFIA RLNSNLLPNE EPFGDVSTTS
160 170 180 190 200
GGSAIEGSDV FLVNGETRSK FYSSERFIDD HRHCISGSAH RVCMILNQYE
210 220 230 240 250
SSSGGPFFRD INSNNGGDYN ALYWYMNSGH VQTESFRTGL HGPYSMYFSR
260 270 280 290 300
SGTPSTNIDT SFFASLGIKG YVAANGRGTV TGKASGADSS MDWVVHWYNN
310 320 330 340 350
DAQYWTYTAS DGSFTSPAMK PGTYTMKYYQ GEFPVAETTV TVSAGSSTTK
360 370 380 390 400
NISGSVKTGT TIFKIGEWDG QPTGFRNADK QLRMHPSDSR MDSWSSTYTV
410 420 430 440 450
GSSSLSDFPM AVFKSVNNPV TIKFTATSAQ TGAATLRIGT TLSFAGGRPQ
460 470 480 490 500
ATINSYTGPA PSAPTNLNSR GVTRGAYRGL GEVYDVSVPA GTIVTGENTI
510 520
TISVISGSSG DAFLSPNVVF DCIELFQ
Length:527
Mass (Da):56,381
Last modified:April 26, 2005 - v1
Checksum:iD07A2C92955DB47D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ490504 mRNA. Translation: ABF50880.1.
AACD01000122 Genomic DNA. Translation: EAA61387.1.
BN001304 Genomic DNA. Translation: CBF79004.1.
RefSeqiXP_664739.1. XM_659647.1.

Genome annotation databases

EnsemblFungiiCADANIAT00000323; CADANIAP00000323; CADANIAG00000323.
EAA61387; EAA61387; AN7135.2.
GeneIDi2870208.
KEGGiani:AN7135.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ490504 mRNA. Translation: ABF50880.1.
AACD01000122 Genomic DNA. Translation: EAA61387.1.
BN001304 Genomic DNA. Translation: CBF79004.1.
RefSeqiXP_664739.1. XM_659647.1.

3D structure databases

ProteinModelPortaliQ5AX45.
SMRiQ5AX45. Positions 21-527.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiPL4. Polysaccharide Lyase Family 4.
mycoCLAPiRGL4A_EMENI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANIAT00000323; CADANIAP00000323; CADANIAG00000323.
EAA61387; EAA61387; AN7135.2.
GeneIDi2870208.
KEGGiani:AN7135.2.

Organism-specific databases

EuPathDBiFungiDB:AN7135.

Phylogenomic databases

HOGENOMiHOG000171008.
InParanoidiQ5AX45.
KOiK18195.
OMAiQPTGFRN.
OrthoDBiEOG092C31LY.

Family and domain databases

CDDicd10316. RGL4_M. 1 hit.
Gene3Di2.60.120.260. 1 hit.
2.60.40.1120. 1 hit.
2.70.98.10. 1 hit.
InterProiIPR013784. Carb-bd-like_fold.
IPR014766. CarboxyPept_regulatory_dom.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR029413. RG-lyase_II.
IPR029411. RG-lyase_III.
IPR016590. Rhamnogalacturonase_B.
IPR015364. RhgB_N.
[Graphical view]
PfamiPF14683. CBM-like. 1 hit.
PF14686. fn3_3. 1 hit.
PF09284. RhgB_N. 1 hit.
[Graphical view]
PIRSFiPIRSF011794. Rhamnogalacturonase_B. 1 hit.
SUPFAMiSSF49452. SSF49452. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF74650. SSF74650. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRGLA_EMENI
AccessioniPrimary (citable) accession number: Q5AX45
Secondary accession number(s): C8VD91, Q1HFS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: April 26, 2005
Last modified: September 7, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.