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Protein

Probable mannan endo-1,4-beta-mannosidase E

Gene

manE

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Endo-1,4-mannanase, a crucial enzyme for depolymerization of seed galactomannans and wood galactoglucomannans.By similarity

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei203Proton donorPROSITE-ProRule annotation1
Active sitei296NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism

Protein family/group databases

CAZyiGH26 Glycoside Hydrolase Family 26

Names & Taxonomyi

Protein namesi
Recommended name:
Probable mannan endo-1,4-beta-mannosidase E (EC:3.2.1.78)
Alternative name(s):
Endo-beta-1,4-mannanase E
Gene namesi
Name:manE
ORF Names:AN7413
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000039507723 – 355Probable mannan endo-1,4-beta-mannosidase EAdd BLAST333

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi50N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi144N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi162N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi287N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ5AWB7

Interactioni

Protein-protein interaction databases

STRINGi162425.CADANIAP00000012

Structurei

3D structure databases

ProteinModelPortaliQ5AWB7
SMRiQ5AWB7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 348GH26PROSITE-ProRule annotationAdd BLAST314

Sequence similaritiesi

Belongs to the glycosyl hydrolase 26 family.PROSITE-ProRule annotationCurated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000235025
InParanoidiQ5AWB7
KOiK01218
OMAiAWWLWFM
OrthoDBiEOG092C2WWM

Family and domain databases

InterProiView protein in InterPro
IPR022790 GH26_dom
IPR000805 Glyco_hydro_26
IPR017853 Glycoside_hydrolase_SF
IPR016714 Mannan-1_4-b-mannosidase
PANTHERiPTHR40079 PTHR40079, 1 hit
PfamiView protein in Pfam
PF02156 Glyco_hydro_26, 1 hit
PIRSFiPIRSF018168 Mannan-1_4-beta-mannosidase, 1 hit
PRINTSiPR00739 GLHYDRLASE26
SUPFAMiSSF51445 SSF51445, 2 hits
PROSITEiView protein in PROSITE
PS51764 GH26, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5AWB7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFSQLILPF SAALSLVGSG VATPTTKPVN PRASRPARNL LAHLVRSAGN
60 70 80 90 100
GTTLSGQQEL KDADWVTDNV GFSPVILGVD LMDYSPSRVE FGAVSTSIED
110 120 130 140 150
AITYATQGGI ITICWHWGEL SHDWMTDERL TTLLTLGSPS GTYNTTEQPW
160 170 180 190 200
WSNFYTEATS FNLSAAMNPA SRDYKLILRD IDAIAEQLAR LKDIPVLFRP
210 220 230 240 250
LHEAEGGWFW WGATGAEPCK ALYRLLFDRL TKKHGLNNLL WVWNSKDPLW
260 270 280 290 300
YPGNEYVDVV SVDVYADNGD HSSQLEAYQA LQGLTGNFSK LIALGEVGNI
310 320 330 340 350
PDPELMREDG AQWAYWVTWN GDFIRGETKN PMEFKKAVYA SELVYTLDEI

QGWNL
Length:355
Mass (Da):39,620
Last modified:April 26, 2005 - v1
Checksum:i37816EA753D5E877
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACD01000128 Genomic DNA Translation: EAA61784.1
BN001304 Genomic DNA Translation: CBF78427.1
RefSeqiXP_680682.1, XM_675590.1

Genome annotation databases

EnsemblFungiiCADANIAT00000012; CADANIAP00000012; CADANIAG00000012
EAA61784; EAA61784; AN7413.2
GeneIDi2869806
KEGGiani:AN7413.2

Entry informationi

Entry nameiMANE_EMENI
AccessioniPrimary (citable) accession number: Q5AWB7
Secondary accession number(s): C8VCC9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: April 26, 2005
Last modified: May 23, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

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