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Q5AUT0 (EGLC_EMENI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable glucan endo-1,3-beta-glucosidase eglC

EC=3.2.1.39
Alternative name(s):
Endo-1,3-beta-glucanase eglC
Laminarinase eglC
Gene names
Name:eglC
ORF Names:AN7950
OrganismEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) [Reference proteome]
Taxonomic identifier227321 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus

Protein attributes

Sequence length465 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Glucanases play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme may be involved in beta-glucan degradation and also function biosynthetically as a transglycosylase. Ref.1

Catalytic activity

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor. Secretedcell wall. Note: Covalently-linked GPI-modified cell wall protein. Ref.4

Induction

Expression is under the control of the nsdD transcription factor required for sexual development. Transcript level is the highest at the beginning of asexual development and decreases thereafter to increase again at the late stage. Ref.1

Post-translational modification

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 17 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 442423Probable glucan endo-1,3-beta-glucosidase eglC
PRO_0000395149
Propeptide443 – 46523Removed in mature form Potential
PRO_0000395150

Regions

Compositional bias310 – 434125Ser-rich

Sites

Active site1281Nucleophile By similarity
Active site2391Proton donor By similarity

Amino acid modifications

Lipidation4421GPI-anchor amidated glycine Potential
Glycosylation1831N-linked (GlcNAc...) Potential
Glycosylation3181N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q5AUT0 [UniParc].

Last modified April 26, 2005. Version 1.
Checksum: C3FA8A043EAED051

FASTA46546,685
        10         20         30         40         50         60 
MFTKTQILAL ALSIASAEAV SKGFNYGANK PDGTLKVQAD FEAEFRTAKN LETTSGFNSA 

        70         80         90        100        110        120 
RLYTMIQGTG STPISAIPAA IAEETTLLLG LWASGGNMDN EIAALKAAIN QYGDEFAKLV 

       130        140        150        160        170        180 
VGISVGSEDL YRNSEIGVQA NAGIGIEPEE LVSYIQRVRE AIAGTALSGA PIGHVDTWNA 

       190        200        210        220        230        240 
WTNGSNAAVA EAVDWLGFDG YPFFQNTMQN SIDDAKALFD ESVQKTKAVA GNKEVWITET 

       250        260        270        280        290        300 
GWPVSGDSQN LAIASVENAK QFWDEVGCPL FDNVNTWWYI LQDASGSSVP NPSFGIVGNT 

       310        320        330        340        350        360 
LSTTPLFDLS CSASSKKNSS SASASASGSS AQSTGFVSTT KPAASPSGSS GLGHGGSLGS 

       370        380        390        400        410        420 
SGSFSGGHYA GVGSSSVIAS PSATPSGSAV PGSSSGPGSS SGSASGSSSG FGSGAAADST 

       430        440        450        460 
SGTSTSGDST SSTSATPADF TGAGSRLSGS IFGAAMLVAA LAVAL 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of the Aspergillus nidulans eglC gene encoding a putative beta-1,3-endoglucanase."
Choi C.J., Ju H.J., Park B.H., Qin R., Jahng K.Y., Han D.M., Chae K.S.
Fungal Genet. Biol. 42:590-600(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION.
[2]"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae."
Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. expand/collapse author list , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.
Nature 438:1105-1115(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[3]"The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G. expand/collapse author list , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
[4]"Comprehensive genomic analysis of cell wall genes in Aspergillus nidulans."
de Groot P.W., Brandt B.W., Horiuchi H., Ram A.F., de Koster C.G., Klis F.M.
Fungal Genet. Biol. 46:S72-S81(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY684801 Genomic DNA. Translation: AAT90341.1.
AACD01000135 Genomic DNA. Translation: EAA59604.1.
BN001302 Genomic DNA. Translation: CBF73583.1.
RefSeqXP_681219.1. XM_676127.1.

3D structure databases

ProteinModelPortalQ5AUT0.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH17. Glycoside Hydrolase Family 17.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCADANIAT00003968; CADANIAP00003968; CADANIAG00003968.
GeneID2869200.
KEGGani:AN7950.2.

Phylogenomic databases

eggNOGCOG5309.
HOGENOMHOG000179527.
OMATNTWWYI.
OrthoDBEOG7TBCCK.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameEGLC_EMENI
AccessionPrimary (citable) accession number: Q5AUT0
Secondary accession number(s): C8V585, Q4QXR7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: April 26, 2005
Last modified: February 19, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries