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Protein

Probable glucan endo-1,3-beta-glucosidase eglC

Gene

eglC

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Glucanases play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation. This enzyme may be involved in beta-glucan degradation and also function biosynthetically as a transglycosylase.1 Publication

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei128NucleophileBy similarity1
Active sitei239Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cell wall biogenesis/degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glucan endo-1,3-beta-glucosidase eglC (EC:3.2.1.39)
Alternative name(s):
Endo-1,3-beta-glucanase eglC
Laminarinase eglC
Gene namesi
Name:eglC
ORF Names:AN7950
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome II
  • UP000005890 Componenti: Partially assembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000039514920 – 442Probable glucan endo-1,3-beta-glucosidase eglCAdd BLAST423
PropeptideiPRO_0000395150443 – 465Removed in mature formSequence analysisAdd BLAST23

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi183N-linked (GlcNAc...)Sequence analysis1
Glycosylationi318N-linked (GlcNAc...)Sequence analysis1
Lipidationi442GPI-anchor amidated glycineSequence analysis1

Post-translational modificationi

The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer (By similarity).By similarity

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PRIDEiQ5AUT0.

Expressioni

Inductioni

Expression is under the control of the nsdD transcription factor required for sexual development. Transcript level is the highest at the beginning of asexual development and decreases thereafter to increase again at the late stage.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ5AUT0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi310 – 434Ser-richAdd BLAST125

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000179527.
InParanoidiQ5AUT0.
KOiK01199.
OMAiTNTWWYI.
OrthoDBiEOG092C3HT4.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5AUT0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFTKTQILAL ALSIASAEAV SKGFNYGANK PDGTLKVQAD FEAEFRTAKN
60 70 80 90 100
LETTSGFNSA RLYTMIQGTG STPISAIPAA IAEETTLLLG LWASGGNMDN
110 120 130 140 150
EIAALKAAIN QYGDEFAKLV VGISVGSEDL YRNSEIGVQA NAGIGIEPEE
160 170 180 190 200
LVSYIQRVRE AIAGTALSGA PIGHVDTWNA WTNGSNAAVA EAVDWLGFDG
210 220 230 240 250
YPFFQNTMQN SIDDAKALFD ESVQKTKAVA GNKEVWITET GWPVSGDSQN
260 270 280 290 300
LAIASVENAK QFWDEVGCPL FDNVNTWWYI LQDASGSSVP NPSFGIVGNT
310 320 330 340 350
LSTTPLFDLS CSASSKKNSS SASASASGSS AQSTGFVSTT KPAASPSGSS
360 370 380 390 400
GLGHGGSLGS SGSFSGGHYA GVGSSSVIAS PSATPSGSAV PGSSSGPGSS
410 420 430 440 450
SGSASGSSSG FGSGAAADST SGTSTSGDST SSTSATPADF TGAGSRLSGS
460
IFGAAMLVAA LAVAL
Length:465
Mass (Da):46,685
Last modified:April 26, 2005 - v1
Checksum:iC3FA8A043EAED051
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY684801 Genomic DNA. Translation: AAT90341.1.
AACD01000135 Genomic DNA. Translation: EAA59604.1.
BN001302 Genomic DNA. Translation: CBF73583.1.
RefSeqiXP_681219.1. XM_676127.1.

Genome annotation databases

EnsemblFungiiCADANIAT00003968; CADANIAP00003968; CADANIAG00003968.
EAA59604; EAA59604; AN7950.2.
GeneIDi2869200.
KEGGiani:AN7950.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY684801 Genomic DNA. Translation: AAT90341.1.
AACD01000135 Genomic DNA. Translation: EAA59604.1.
BN001302 Genomic DNA. Translation: CBF73583.1.
RefSeqiXP_681219.1. XM_676127.1.

3D structure databases

ProteinModelPortaliQ5AUT0.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Proteomic databases

PRIDEiQ5AUT0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANIAT00003968; CADANIAP00003968; CADANIAG00003968.
EAA59604; EAA59604; AN7950.2.
GeneIDi2869200.
KEGGiani:AN7950.2.

Phylogenomic databases

HOGENOMiHOG000179527.
InParanoidiQ5AUT0.
KOiK01199.
OMAiTNTWWYI.
OrthoDBiEOG092C3HT4.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiEGLC_EMENI
AccessioniPrimary (citable) accession number: Q5AUT0
Secondary accession number(s): C8V585, Q4QXR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: April 26, 2005
Last modified: November 30, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.