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Protein

Exopolygalacturonase X-1

Gene

pgaX-1

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Specific in hydrolyzing the terminal glycosidic bond of polygalacturonic acid and oligogalacturonates.1 Publication

Catalytic activityi

((1->4)-alpha-D-galacturonide)(n) + H2O = ((1->4)-alpha-D-galacturonide)(n-1) + D-galacturonate.

pH dependencei

Optimum pH is 4.4.1 Publication

Temperature dependencei

Optimum temperature is 48 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei245 – 2451Proton donorPROSITE-ProRule annotation
Active sitei268 – 2681PROSITE-ProRule annotation

GO - Molecular functioni

  • galacturan 1,4-alpha-galacturonidase activity Source: UniProtKB-EC
  • polygalacturonase activity Source: UniProtKB

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • pectin catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Exopolygalacturonase X-1 (EC:3.2.1.67)
Short name:
ExoPG
Alternative name(s):
Galacturan 1,4-alpha-galacturonidase
Poly(1,4-alpha-D-galacturonide)galacturonohydrolase
Gene namesi
Name:pgaX-1
ORF Names:AN8761
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome III
  • UP000005890 Componenti: Partially assembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:AN8761.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 434412Exopolygalacturonase X-1PRO_0000393672Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence analysis
Glycosylationi129 – 1291N-linked (GlcNAc...)Sequence analysis
Glycosylationi199 – 1991N-linked (GlcNAc...)Sequence analysis
Disulfide bondi247 ↔ 264By similarity
Glycosylationi253 – 2531N-linked (GlcNAc...)Sequence analysis
Glycosylationi265 – 2651N-linked (GlcNAc...)Sequence analysis
Glycosylationi292 – 2921N-linked (GlcNAc...)Sequence analysis
Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence analysis
Glycosylationi329 – 3291N-linked (GlcNAc...)Sequence analysis
Glycosylationi354 – 3541N-linked (GlcNAc...)Sequence analysis
Glycosylationi364 – 3641N-linked (GlcNAc...)Sequence analysis
Disulfide bondi392 ↔ 398By similarity
Glycosylationi423 – 4231N-linked (GlcNAc...)Sequence analysis
Glycosylationi430 – 4301N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliQ5ASG9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati231 – 25222PbH1 1Add
BLAST
Repeati254 – 27421PbH1 2Add
BLAST
Repeati327 – 34822PbH1 3Add
BLAST
Repeati362 – 40544PbH1 4Add
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated
Contains 4 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000217379.
InParanoidiQ5ASG9.
KOiK01213.
OMAiNSHIDNG.
OrthoDBiEOG7R83B4.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 4 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5ASG9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSHLLTSA VSVLSLGLTV EGHFSRSRND AVGPKRPFKP LPYSHPRKKV
60 70 80 90 100
CHVRSHGDGR DDSAFILSAL KSCNNGGKVV FAEEKEYTIG TALDLTFLKH
110 120 130 140 150
VDLEILGRIQ FTNDTDYWQA NSFKHTFQNA TTFFQLGGED VNVYGGGTLD
160 170 180 190 200
GNGQIWYDLY AEDPLILRPI LFGVIGLHGG TIGPLKLRYS PQWYQLVANS
210 220 230 240 250
SDVLFDGIDI SGYSKSENEA KNTDGWDTYR SKNIVIQNSV INNGDDCVSF
260 270 280 290 300
KPNSTEILVQ NLYCNGSHGI SVGSLGQYIG EVDIVKNVLV YNISMYNASD
310 320 330 340 350
MARIKVWPGV ASAMSEDLQG GGGLGSVSNI TYEDMYIENV DWAIEITQCY
360 370 380 390 400
GQKNMTLCNE YPSNLTISDV YISNMYGTTS SARDPNIGTI VCSSPDVCSN
410 420 430
IYVENIDVVS PSGTNDFICT NVNESLLQVN CTSG
Length:434
Mass (Da):47,537
Last modified:April 26, 2005 - v1
Checksum:i87562E501658A958
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ490519 mRNA. Translation: ABF50895.1.
AACD01000161 Genomic DNA. Translation: EAA60554.1.
BN001303 Genomic DNA. Translation: CBF78078.1.
RefSeqiXP_682030.1. XM_676938.1.

Genome annotation databases

EnsemblFungiiCADANIAT00006304; CADANIAP00006304; CADANIAG00006304.
EAA60554; EAA60554; AN8761.2.
GeneIDi2868323.
KEGGiani:AN8761.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ490519 mRNA. Translation: ABF50895.1.
AACD01000161 Genomic DNA. Translation: EAA60554.1.
BN001303 Genomic DNA. Translation: CBF78078.1.
RefSeqiXP_682030.1. XM_676938.1.

3D structure databases

ProteinModelPortaliQ5ASG9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANIAT00006304; CADANIAP00006304; CADANIAG00006304.
EAA60554; EAA60554; AN8761.2.
GeneIDi2868323.
KEGGiani:AN8761.2.

Organism-specific databases

EuPathDBiFungiDB:AN8761.

Phylogenomic databases

HOGENOMiHOG000217379.
InParanoidiQ5ASG9.
KOiK01213.
OMAiNSHIDNG.
OrthoDBiEOG7R83B4.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 4 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Development and application of a suite of polysaccharide-degrading enzymes for analyzing plant cell walls."
    Bauer S., Vasu P., Persson S., Mort A.J., Somerville C.R.
    Proc. Natl. Acad. Sci. U.S.A. 103:11417-11422(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.
  3. "The 2008 update of the Aspergillus nidulans genome annotation: a community effort."
    Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J., Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H., Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M., Estrada C.G.
    , Geysens S., Goldman G., de Groot P.W., Hansen K., Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G., Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L., Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M., van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P., Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J., Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A., Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X., Robson G., Seiboth B., van Solingen P., Specht T., Sun J., Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H., van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y., Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J., Oliver S.G., Turner G.
    Fungal Genet. Biol. 46:S2-13(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139.

Entry informationi

Entry nameiPGLX1_EMENI
AccessioniPrimary (citable) accession number: Q5ASG9
Secondary accession number(s): C8V9X8, Q1HFQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: April 26, 2005
Last modified: April 13, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.