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Protein

Exopolygalacturonase X-1

Gene

pgaX-1

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Specific in hydrolyzing the terminal glycosidic bond of polygalacturonic acid and oligogalacturonates.1 Publication

Catalytic activityi

((1->4)-alpha-D-galacturonide)(n) + H2O = ((1->4)-alpha-D-galacturonide)(n-1) + D-galacturonate.

pH dependencei

Optimum pH is 4.4.1 Publication

Temperature dependencei

Optimum temperature is 48 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei245Proton donorPROSITE-ProRule annotation1
Active sitei268PROSITE-ProRule annotation1

GO - Molecular functioni

  • galacturan 1,4-alpha-galacturonidase activity Source: UniProtKB-EC
  • polygalacturonase activity Source: UniProtKB

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • pectin catabolic process Source: UniProtKB
Complete GO annotation...

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCell wall biogenesis/degradation

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Exopolygalacturonase X-1 (EC:3.2.1.67)
Short name:
ExoPG
Alternative name(s):
Galacturan 1,4-alpha-galacturonidase
Poly(1,4-alpha-D-galacturonide)galacturonohydrolase
Gene namesi
Name:pgaX-1
ORF Names:AN8761
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome III
  • UP000005890 Componenti: Partially assembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000039367223 – 434Exopolygalacturonase X-1Add BLAST412

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi113N-linked (GlcNAc...)Sequence analysis1
Glycosylationi129N-linked (GlcNAc...)Sequence analysis1
Glycosylationi199N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi247 ↔ 264By similarity
Glycosylationi253N-linked (GlcNAc...)Sequence analysis1
Glycosylationi265N-linked (GlcNAc...)Sequence analysis1
Glycosylationi292N-linked (GlcNAc...)Sequence analysis1
Glycosylationi297N-linked (GlcNAc...)Sequence analysis1
Glycosylationi329N-linked (GlcNAc...)Sequence analysis1
Glycosylationi354N-linked (GlcNAc...)Sequence analysis1
Glycosylationi364N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi392 ↔ 398By similarity
Glycosylationi423N-linked (GlcNAc...)Sequence analysis1
Glycosylationi430N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ5ASG9.

Structurei

3D structure databases

ProteinModelPortaliQ5ASG9.
SMRiQ5ASG9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati231 – 252PbH1 1Add BLAST22
Repeati254 – 274PbH1 2Add BLAST21
Repeati327 – 348PbH1 3Add BLAST22
Repeati362 – 405PbH1 4Add BLAST44

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated
Contains 4 PbH1 repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000217379.
InParanoidiQ5ASG9.
KOiK01213.
OMAiRNDACKP.
OrthoDBiEOG092C2CYK.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 4 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5ASG9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSHLLTSA VSVLSLGLTV EGHFSRSRND AVGPKRPFKP LPYSHPRKKV
60 70 80 90 100
CHVRSHGDGR DDSAFILSAL KSCNNGGKVV FAEEKEYTIG TALDLTFLKH
110 120 130 140 150
VDLEILGRIQ FTNDTDYWQA NSFKHTFQNA TTFFQLGGED VNVYGGGTLD
160 170 180 190 200
GNGQIWYDLY AEDPLILRPI LFGVIGLHGG TIGPLKLRYS PQWYQLVANS
210 220 230 240 250
SDVLFDGIDI SGYSKSENEA KNTDGWDTYR SKNIVIQNSV INNGDDCVSF
260 270 280 290 300
KPNSTEILVQ NLYCNGSHGI SVGSLGQYIG EVDIVKNVLV YNISMYNASD
310 320 330 340 350
MARIKVWPGV ASAMSEDLQG GGGLGSVSNI TYEDMYIENV DWAIEITQCY
360 370 380 390 400
GQKNMTLCNE YPSNLTISDV YISNMYGTTS SARDPNIGTI VCSSPDVCSN
410 420 430
IYVENIDVVS PSGTNDFICT NVNESLLQVN CTSG
Length:434
Mass (Da):47,537
Last modified:April 26, 2005 - v1
Checksum:i87562E501658A958
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ490519 mRNA. Translation: ABF50895.1.
AACD01000161 Genomic DNA. Translation: EAA60554.1.
BN001303 Genomic DNA. Translation: CBF78078.1.
RefSeqiXP_682030.1. XM_676938.1.

Genome annotation databases

EnsemblFungiiCADANIAT00006304; CADANIAP00006304; CADANIAG00006304.
EAA60554; EAA60554; AN8761.2.
GeneIDi2868323.
KEGGiani:AN8761.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ490519 mRNA. Translation: ABF50895.1.
AACD01000161 Genomic DNA. Translation: EAA60554.1.
BN001303 Genomic DNA. Translation: CBF78078.1.
RefSeqiXP_682030.1. XM_676938.1.

3D structure databases

ProteinModelPortaliQ5ASG9.
SMRiQ5ASG9.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Proteomic databases

PRIDEiQ5ASG9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANIAT00006304; CADANIAP00006304; CADANIAG00006304.
EAA60554; EAA60554; AN8761.2.
GeneIDi2868323.
KEGGiani:AN8761.2.

Phylogenomic databases

HOGENOMiHOG000217379.
InParanoidiQ5ASG9.
KOiK01213.
OMAiRNDACKP.
OrthoDBiEOG092C2CYK.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 4 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGLX1_EMENI
AccessioniPrimary (citable) accession number: Q5ASG9
Secondary accession number(s): C8V9X8, Q1HFQ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: April 26, 2005
Last modified: November 30, 2016
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.