Q5APF2 (GUAA_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing]
EC=6.3.5.2

Alternative name(s):
GMP synthetase
Glutamine amidotransferase

Gene names

Name:	GUA1
ORF Names:	CaO19.4813, CaO19.12276

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell soluble fraction Inferred from direct assay. Source: CGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 530	530	GMP synthase [glutamine-hydrolyzing]		PRO_0000286146
Regions
	Domain	18 – 207	190	Glutamine amidotransferase type-1
	Domain	208 – 405	198	GMPS ATP-PPase
	Nucleotide binding	236 – 242	7	ATP By similarity
Sites
	Active site	94	1	For GATase activity By similarity
	Active site	181	1	For GATase activity By similarity
	Active site	183	1	For GATase activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5APF2 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 87BCD288C209DF46	FASTA	530	58,813
	10 20 30 40 50 60 MSANIDDVPI EVSKVFDTIL VLDFGSQYSH LITRRLREFN VYAEMLPCTQ KIAELSWKPK 70 80 90 100 110 120 GIILSGGPYS VYAEDAPHVD HDIFKLGVPI LGICYGMQEL AWINGKGVAR GDKREYGPAT 130 140 150 160 170 180 LNVEDPECAL FKGVDHSQVW MSHGDKLHAL PTGFKVVATS DNSPFCGISN ESEHIYGIQF 190 200 210 220 230 240 HPEVTHTVQG KKLLKNFAVD ICQAKTNWSM ENFIDTEIAR IKKLVGPTAE VIGAVSGGVD 250 260 270 280 290 300 STVGAKIMKE AIGDRFHAIY VDNGVMRKNE TESVKKTLDE GLGINLTVVD AGDLFLGRLK 310 320 330 340 350 360 GVTDPEKKRK IIGNTFIHVF EEEAAKIKPR DGSEIEYLLQ GTLYPDVIES ISFKGPSQTI 370 380 390 400 410 420 KTHHNVGGLL EDMKLKLIEP LRELFKDEVR HLGELLGVPE DLVWRHPFPG PGLAIRVLGE 430 440 450 460 470 480 VTKEQVKIAR EADAIFIEEI KKAGLYRQIS QAFAALLPVK SVGVMGDQRT YEQVIALRAI 490 500 510 520 530 ETLDFMTADW FIFEAAFLKK VASRIVNEVD GVARVTYDIT SKPPATVEWE

References

[1]

"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACQ01000002 Genomic DNA. Translation: EAL04605.1. AACQ01000001 Genomic DNA. Translation: EAL04801.1.
RefSeq	XP_723309.1. XM_718216.1. XP_723499.1. XM_718406.1.
3D structure databases
HSSP	HSSP built from PDB template 1GPM based on UniProtKB P04079.
ProteinModelPortal	Q5APF2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5APF2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3634763. 3634968.
KEGG	cal:CaO19.12276. cal:CaO19.4813.
Organism-specific databases
CGD	CAL0005313. GUA1.
Phylogenomic databases
OMA	DGRTYEY.
PhylomeDB	Q5APF2.
Family and domain databases
InterPro	IPR017926. GATASE_1. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit. TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA_CANAL

Accession

Primary (citable) accession number: Q5APF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	April 26, 2005
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program