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Q5AMT2

- BGL2_CANAL

UniProt

Q5AMT2 - BGL2_CANAL

Protein

Glucan 1,3-beta-glucosidase BGL2

Gene

BGL2

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (26 Apr 2005)
      Previous versions | rss
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    Functioni

    Cell wall glucan 1,3-beta-glucosidase involved in cell wall biosynthesis and virulence. Crucial for delivery of beta-1,3-glucan to the biofilm matrix and for accumulation of mature matrix biomass. Plays a role as a major antigen in human systemic candidiasis patients.3 Publications

    Catalytic activityi

    Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei228 – 2281NucleophileBy similarity
    Active sitei287 – 2871Proton donorBy similarity

    GO - Molecular functioni

    1. 1,3-beta-glucanosyltransferase activity Source: CGD
    2. glucan exo-1,3-beta-glucosidase activity Source: UniProtKB-EC
    3. glucanosyltransferase activity Source: CGD

    GO - Biological processi

    1. adhesion of symbiont to host Source: CGD
    2. carbohydrate metabolic process Source: InterPro
    3. fungal-type cell wall organization Source: CGD
    4. growth of symbiont in host Source: CGD
    5. pathogenesis Source: CGD
    6. single-species biofilm formation in or on host organism Source: CGD
    7. single-species biofilm formation on inanimate substrate Source: CGD

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation, Virulence

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucan 1,3-beta-glucosidase BGL2 (EC:3.2.1.58)
    Alternative name(s):
    Exo-1,3-beta-glucanase
    Gene namesi
    Name:BGL2
    Synonyms:BGL21
    ORF Names:CaO19.12034, CaO19.4565
    OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
    Taxonomic identifieri237561 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida
    ProteomesiUP000000559: Unassembled WGS sequence

    Organism-specific databases

    CGDiCAL0002830. BGL2.

    Subcellular locationi

    Secretedcell wall 1 Publication. Cytoplasm 1 Publication
    Note: Tightly bound to cell wall. A fraction of unprecessed protein is found in the cytoplasm.

    GO - Cellular componenti

    1. cell surface Source: CGD
    2. cytoplasm Source: UniProtKB-SubCell
    3. extracellular region Source: CGD
    4. fungal-type cell wall Source: CGD
    5. yeast-form cell wall Source: CGD

    Keywords - Cellular componenti

    Cell wall, Cytoplasm, Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Exhibits diminished extracellular biofilm material, renders cells more dependent on chitin for wall integrity, and attenuates virulence in mice.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 18181 PublicationAdd
    BLAST
    Chaini19 – 308290Glucan 1,3-beta-glucosidase BGL2PRO_0000428632Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi197 – 1971N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Inductioni

    Induced during biofilm formation and by fluconazole and micafungin. Expression is also regulated by RCK2.4 Publications

    Interactioni

    Protein-protein interaction databases

    STRINGi5476.CAL0002830.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5AMT2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 17 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOGENOMiHOG000207150.
    KOiK01210.
    OrthoDBiEOG7WMCVT.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00332. Glyco_hydro_17. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5AMT2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQIKFLTTLA TVLTSVAAMG DLAFNLGVKN DDGTCKDVST FEGDLDFLKS    50
    HSKIIKTYAV SDCNTLQNLG PAAEAEGFQI QLGIWPNDDA HFEAEKEALQ 100
    NYLPKISVST IKIFLVGSEA LYREDLTASE LASKINDIKD LVKGIKDKNG 150
    KSYSSVPVGT VDSWNVLVDG ASKPAIDAAD VVYSNSFSYW QKNSQANASY 200
    SLFDDVMQAL QTLQTAKGST DIEFWVGETG WPTDGSSYGD SVPSVENAAD 250
    QWQKGICALR AWGINVAVYE AFDEAWKPDT SGTSSVEKHW GVWQSDKTLK 300
    YSIDCKFN 308
    Length:308
    Mass (Da):33,656
    Last modified:April 26, 2005 - v1
    Checksum:i10E0C6601080A6C1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACQ01000006 Genomic DNA. Translation: EAL03900.1.
    AACQ01000005 Genomic DNA. Translation: EAL04054.1.
    RefSeqiXP_722637.1. XM_717544.1.
    XP_722782.1. XM_717689.1.

    Genome annotation databases

    GeneIDi3635482.
    3635691.
    KEGGical:CaO19.12034.
    cal:CaO19.4565.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AACQ01000006 Genomic DNA. Translation: EAL03900.1 .
    AACQ01000005 Genomic DNA. Translation: EAL04054.1 .
    RefSeqi XP_722637.1. XM_717544.1.
    XP_722782.1. XM_717689.1.

    3D structure databases

    ProteinModelPortali Q5AMT2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 5476.CAL0002830.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3635482.
    3635691.
    KEGGi cal:CaO19.12034.
    cal:CaO19.4565.

    Organism-specific databases

    CGDi CAL0002830. BGL2.

    Phylogenomic databases

    HOGENOMi HOG000207150.
    KOi K01210.
    OrthoDBi EOG7WMCVT.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00332. Glyco_hydro_17. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: SC5314 / ATCC MYA-2876.
    2. Cited for: PROTEIN SEQUENCE OF 19-26, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, INDUCTION.
    3. "Kinetics of beta-1,3 glucan interaction at the donor and acceptor sites of the fungal glucosyltransferase encoded by the BGL2 gene."
      Goldman R.C., Sullivan P.A., Zakula D., Capobianco J.O.
      Eur. J. Biochem. 227:372-378(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY.
    4. "Phenotype in Candida albicans of a disruption of the BGL2 gene encoding a 1,3-beta-glucosyltransferase."
      Sarthy A.V., McGonigal T., Coen M., Frost D.J., Meulbroek J.A., Goldman R.C.
      Microbiology 143:367-376(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    5. "Two-dimensional gel electrophoresis as analytical tool for identifying Candida albicans immunogenic proteins."
      Pitarch A., Pardo M., Jimenez A., Pla J., Gil C., Sanchez M., Nombela C.
      Electrophoresis 20:1001-1010(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION AS AN IMMUNOGENIC PROTEIN.
    6. "Exposure of Candida albicans to antifungal agents affects expression of SAP2 and SAP9 secreted proteinase genes."
      Copping V.M.S., Barelle C.J., Hube B., Gow N.A.R., Brown A.J.P., Odds F.C.
      J. Antimicrob. Chemother. 55:645-654(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    7. "Decoding serological response to Candida cell wall immunome into novel diagnostic, prognostic, and therapeutic candidates for systemic candidiasis by proteomic and bioinformatic analyses."
      Pitarch A., Jimenez A., Nombela C., Gil C.
      Mol. Cell. Proteomics 5:79-96(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION AS AN IMMUNOGENIC PROTEIN.
    8. "Immunoglobulin G responses to a panel of Candida albicans antigens as accurate and early markers for the presence of systemic candidiasis."
      Clancy C.J., Nguyen M.L., Cheng S., Huang H., Fan G., Jaber R.A., Wingard J.R., Cline C., Nguyen M.H.
      J. Clin. Microbiol. 46:1647-1654(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION AS AN IMMUNOGENIC PROTEIN.
    9. "Time course global gene expression analysis of an in vivo Candida biofilm."
      Nett J.E., Lepak A.J., Marchillo K., Andes D.R.
      J. Infect. Dis. 200:307-313(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    10. "The MAP kinase-activated protein kinase Rck2p regulates cellular responses to cell wall stresses, filamentation and virulence in the human fungal pathogen Candida albicans."
      Li X., Du W., Zhao J., Zhang L., Zhu Z., Jiang L.
      FEMS Yeast Res. 10:441-451(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    11. "A Candida biofilm-induced pathway for matrix glucan delivery: implications for drug resistance."
      Taff H.T., Nett J.E., Zarnowski R., Ross K.M., Sanchez H., Cain M.T., Hamaker J., Mitchell A.P., Andes D.R.
      PLoS Pathog. 8:E1002848-E1002848(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiBGL2_CANAL
    AccessioniPrimary (citable) accession number: Q5AMT2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 16, 2014
    Last sequence update: April 26, 2005
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Candida albicans
      Candida albicans: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3