Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q5AMT2

- BGL2_CANAL

UniProt

Q5AMT2 - BGL2_CANAL

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Glucan 1,3-beta-glucosidase BGL2

Gene

BGL2

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cell wall glucan 1,3-beta-glucosidase involved in cell wall biosynthesis and virulence. Crucial for delivery of beta-1,3-glucan to the biofilm matrix and for accumulation of mature matrix biomass. Plays a role as a major antigen in human systemic candidiasis patients.3 Publications

Catalytic activityi

Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei228 – 2281NucleophileBy similarity
Active sitei287 – 2871Proton donorBy similarity

GO - Molecular functioni

  1. 1,3-beta-glucanosyltransferase activity Source: CGD
  2. glucan exo-1,3-beta-glucosidase activity Source: UniProtKB-EC
  3. glucanosyltransferase activity Source: CGD

GO - Biological processi

  1. adhesion of symbiont to host Source: CGD
  2. carbohydrate metabolic process Source: InterPro
  3. fungal-type cell wall organization Source: CGD
  4. growth of symbiont in host Source: CGD
  5. pathogenesis Source: CGD
  6. single-species biofilm formation in or on host organism Source: CGD
  7. single-species biofilm formation on inanimate substrate Source: CGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation, Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan 1,3-beta-glucosidase BGL2 (EC:3.2.1.58)
Alternative name(s):
Exo-1,3-beta-glucanase
Gene namesi
Name:BGL2
Synonyms:BGL21
ORF Names:CaO19.12034, CaO19.4565
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
ProteomesiUP000000559: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0002830. BGL2.

Subcellular locationi

Secretedcell wall 1 Publication. Cytoplasm 1 Publication
Note: Tightly bound to cell wall. A fraction of unprecessed protein is found in the cytoplasm.

GO - Cellular componenti

  1. cell surface Source: CGD
  2. cytoplasm Source: UniProtKB-KW
  3. extracellular region Source: CGD
  4. fungal-type cell wall Source: CGD
  5. yeast-form cell wall Source: CGD
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Cytoplasm, Secreted

Pathology & Biotechi

Disruption phenotypei

Exhibits diminished extracellular biofilm material, renders cells more dependent on chitin for wall integrity, and attenuates virulence in mice.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 18181 PublicationAdd
BLAST
Chaini19 – 308290Glucan 1,3-beta-glucosidase BGL2PRO_0000428632Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi197 – 1971N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Inductioni

Induced during biofilm formation and by fluconazole and micafungin. Expression is also regulated by RCK2.4 Publications

Interactioni

Protein-protein interaction databases

STRINGi5476.CAL0002830.

Structurei

3D structure databases

ProteinModelPortaliQ5AMT2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000207150.
InParanoidiQ5AMT2.
KOiK01210.
OrthoDBiEOG7WMCVT.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5AMT2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQIKFLTTLA TVLTSVAAMG DLAFNLGVKN DDGTCKDVST FEGDLDFLKS
60 70 80 90 100
HSKIIKTYAV SDCNTLQNLG PAAEAEGFQI QLGIWPNDDA HFEAEKEALQ
110 120 130 140 150
NYLPKISVST IKIFLVGSEA LYREDLTASE LASKINDIKD LVKGIKDKNG
160 170 180 190 200
KSYSSVPVGT VDSWNVLVDG ASKPAIDAAD VVYSNSFSYW QKNSQANASY
210 220 230 240 250
SLFDDVMQAL QTLQTAKGST DIEFWVGETG WPTDGSSYGD SVPSVENAAD
260 270 280 290 300
QWQKGICALR AWGINVAVYE AFDEAWKPDT SGTSSVEKHW GVWQSDKTLK

YSIDCKFN
Length:308
Mass (Da):33,656
Last modified:April 26, 2005 - v1
Checksum:i10E0C6601080A6C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000006 Genomic DNA. Translation: EAL03900.1.
AACQ01000005 Genomic DNA. Translation: EAL04054.1.
RefSeqiXP_722637.1. XM_717544.1.
XP_722782.1. XM_717689.1.

Genome annotation databases

GeneIDi3635482.
3635691.
KEGGical:CaO19.12034.
cal:CaO19.4565.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000006 Genomic DNA. Translation: EAL03900.1 .
AACQ01000005 Genomic DNA. Translation: EAL04054.1 .
RefSeqi XP_722637.1. XM_717544.1.
XP_722782.1. XM_717689.1.

3D structure databases

ProteinModelPortali Q5AMT2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 5476.CAL0002830.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3635482.
3635691.
KEGGi cal:CaO19.12034.
cal:CaO19.4565.

Organism-specific databases

CGDi CAL0002830. BGL2.

Phylogenomic databases

HOGENOMi HOG000207150.
InParanoidi Q5AMT2.
KOi K01210.
OrthoDBi EOG7WMCVT.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00332. Glyco_hydro_17. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC5314 / ATCC MYA-2876.
  2. Cited for: PROTEIN SEQUENCE OF 19-26, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, INDUCTION.
  3. "Kinetics of beta-1,3 glucan interaction at the donor and acceptor sites of the fungal glucosyltransferase encoded by the BGL2 gene."
    Goldman R.C., Sullivan P.A., Zakula D., Capobianco J.O.
    Eur. J. Biochem. 227:372-378(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.
  4. "Phenotype in Candida albicans of a disruption of the BGL2 gene encoding a 1,3-beta-glucosyltransferase."
    Sarthy A.V., McGonigal T., Coen M., Frost D.J., Meulbroek J.A., Goldman R.C.
    Microbiology 143:367-376(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  5. "Two-dimensional gel electrophoresis as analytical tool for identifying Candida albicans immunogenic proteins."
    Pitarch A., Pardo M., Jimenez A., Pla J., Gil C., Sanchez M., Nombela C.
    Electrophoresis 20:1001-1010(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION AS AN IMMUNOGENIC PROTEIN.
  6. "Exposure of Candida albicans to antifungal agents affects expression of SAP2 and SAP9 secreted proteinase genes."
    Copping V.M.S., Barelle C.J., Hube B., Gow N.A.R., Brown A.J.P., Odds F.C.
    J. Antimicrob. Chemother. 55:645-654(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "Decoding serological response to Candida cell wall immunome into novel diagnostic, prognostic, and therapeutic candidates for systemic candidiasis by proteomic and bioinformatic analyses."
    Pitarch A., Jimenez A., Nombela C., Gil C.
    Mol. Cell. Proteomics 5:79-96(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION AS AN IMMUNOGENIC PROTEIN.
  8. "Immunoglobulin G responses to a panel of Candida albicans antigens as accurate and early markers for the presence of systemic candidiasis."
    Clancy C.J., Nguyen M.L., Cheng S., Huang H., Fan G., Jaber R.A., Wingard J.R., Cline C., Nguyen M.H.
    J. Clin. Microbiol. 46:1647-1654(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION AS AN IMMUNOGENIC PROTEIN.
  9. "Time course global gene expression analysis of an in vivo Candida biofilm."
    Nett J.E., Lepak A.J., Marchillo K., Andes D.R.
    J. Infect. Dis. 200:307-313(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  10. "The MAP kinase-activated protein kinase Rck2p regulates cellular responses to cell wall stresses, filamentation and virulence in the human fungal pathogen Candida albicans."
    Li X., Du W., Zhao J., Zhang L., Zhu Z., Jiang L.
    FEMS Yeast Res. 10:441-451(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  11. "A Candida biofilm-induced pathway for matrix glucan delivery: implications for drug resistance."
    Taff H.T., Nett J.E., Zarnowski R., Ross K.M., Sanchez H., Cain M.T., Hamaker J., Mitchell A.P., Andes D.R.
    PLoS Pathog. 8:E1002848-E1002848(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiBGL2_CANAL
AccessioniPrimary (citable) accession number: Q5AMT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 26, 2005
Last modified: October 29, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3