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Protein

Glucan 1,3-beta-glucosidase BGL2

Gene

BGL2

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell wall glucan 1,3-beta-glucosidase involved in cell wall biosynthesis and virulence. Crucial for delivery of beta-1,3-glucan to the biofilm matrix and for accumulation of mature matrix biomass. Plays a role as a major antigen in human systemic candidiasis patients.3 Publications

Catalytic activityi

Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1->3)-beta-D-glucans, releasing alpha-glucose.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei228NucleophileBy similarity1
Active sitei287Proton donorBy similarity1

GO - Molecular functioni

  • 1,3-beta-glucanosyltransferase activity Source: CGD
  • glucan exo-1,3-beta-glucosidase activity Source: UniProtKB-EC
  • glucanosyltransferase activity Source: CGD

GO - Biological processi

  • adhesion of symbiont to host Source: CGD
  • carbohydrate metabolic process Source: InterPro
  • fungal-type cell wall organization Source: CGD
  • growth of symbiont in host Source: CGD
  • pathogenesis Source: CGD
  • single-species biofilm formation in or on host organism Source: CGD
  • single-species biofilm formation on inanimate substrate Source: CGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation, Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan 1,3-beta-glucosidase BGL2 (EC:3.2.1.58)
Alternative name(s):
Exo-1,3-beta-glucanase
Gene namesi
Name:BGL2
Synonyms:BGL21
ORF Names:CaO19.12034, CaO19.4565
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
Proteomesi
  • UP000000559 Componenti: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0000186956. BGL2.

Subcellular locationi

GO - Cellular componenti

  • cell surface Source: CGD
  • cytoplasm Source: UniProtKB-SubCell
  • extracellular region Source: CGD
  • fungal-type cell wall Source: CGD
  • yeast-form cell wall Source: CGD
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Cytoplasm, Secreted

Pathology & Biotechi

Disruption phenotypei

Exhibits diminished extracellular biofilm material, renders cells more dependent on chitin for wall integrity, and attenuates virulence in mice.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 181 PublicationAdd BLAST18
ChainiPRO_000042863219 – 308Glucan 1,3-beta-glucosidase BGL2Add BLAST290

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi197N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ5AMT2.

Expressioni

Inductioni

Induced during biofilm formation and by fluconazole and micafungin. Expression is also regulated by RCK2.4 Publications

Structurei

3D structure databases

ProteinModelPortaliQ5AMT2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000207150.
InParanoidiQ5AMT2.
KOiK01210.
OrthoDBiEOG092C3V78.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5AMT2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIKFLTTLA TVLTSVAAMG DLAFNLGVKN DDGTCKDVST FEGDLDFLKS
60 70 80 90 100
HSKIIKTYAV SDCNTLQNLG PAAEAEGFQI QLGIWPNDDA HFEAEKEALQ
110 120 130 140 150
NYLPKISVST IKIFLVGSEA LYREDLTASE LASKINDIKD LVKGIKDKNG
160 170 180 190 200
KSYSSVPVGT VDSWNVLVDG ASKPAIDAAD VVYSNSFSYW QKNSQANASY
210 220 230 240 250
SLFDDVMQAL QTLQTAKGST DIEFWVGETG WPTDGSSYGD SVPSVENAAD
260 270 280 290 300
QWQKGICALR AWGINVAVYE AFDEAWKPDT SGTSSVEKHW GVWQSDKTLK

YSIDCKFN
Length:308
Mass (Da):33,656
Last modified:April 26, 2005 - v1
Checksum:i10E0C6601080A6C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000006 Genomic DNA. Translation: EAL03900.1.
AACQ01000005 Genomic DNA. Translation: EAL04054.1.
RefSeqiXP_722637.1. XM_717544.1.
XP_722782.1. XM_717689.1.

Genome annotation databases

EnsemblFungiiEAL03900; EAL03900; CaO19.4565.
EAL04054; EAL04054; CaO19.12034.
GeneIDi3635482.
3635691.
KEGGical:CaO19.12034.
cal:CaO19.4565.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000006 Genomic DNA. Translation: EAL03900.1.
AACQ01000005 Genomic DNA. Translation: EAL04054.1.
RefSeqiXP_722637.1. XM_717544.1.
XP_722782.1. XM_717689.1.

3D structure databases

ProteinModelPortaliQ5AMT2.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ5AMT2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAL03900; EAL03900; CaO19.4565.
EAL04054; EAL04054; CaO19.12034.
GeneIDi3635482.
3635691.
KEGGical:CaO19.12034.
cal:CaO19.4565.

Organism-specific databases

CGDiCAL0000186956. BGL2.

Phylogenomic databases

HOGENOMiHOG000207150.
InParanoidiQ5AMT2.
KOiK01210.
OrthoDBiEOG092C3V78.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGL2_CANAL
AccessioniPrimary (citable) accession number: Q5AMT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: April 26, 2005
Last modified: October 5, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.