Q5AL27 (AKR1_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Palmitoyltransferase AKR1
EC=2.3.1.-

Alternative name(s):
Ankyrin repeat-containing protein AKR1

Gene names

Name:	AKR1
ORF Names:	CaO19.4950, CaO19.12414

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	813 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Palmitoyltransferase specific for casein kinase 1 By similarity.
Catalytic activity	Palmitoyl-CoA + protein-cysteine = S-palmitoyl protein + CoA.
Subcellular location	Early endosome membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein By similarity.
Domain	The DHHC domain is required for palmitoyltransferase activity By similarity.
Sequence similarities	Belongs to the DHHC palmitoyltransferase family. AKR/ZDHHC17 subfamily. Contains 6 ANK repeats. Contains 1 DHHC-type zinc finger.

Ontologies

Keywords
Cellular component	Endosome Golgi apparatus Membrane
Domain	ANK repeat Repeat Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell early endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 813	813	Palmitoyltransferase AKR1		PRO_0000212919
Regions
	Topological domain	1 – 387	387	Cytoplasmic Potential
	Transmembrane	388 – 408	21	Helical; Potential
	Topological domain	409 – 412	4	Lumenal Potential
	Transmembrane	413 – 433	21	Helical; Potential
	Topological domain	434 – 452	19	Cytoplasmic Potential
	Transmembrane	453 – 473	21	Helical; Potential
	Topological domain	474 – 485	12	Lumenal Potential
	Transmembrane	486 – 506	21	Helical; Potential
	Topological domain	507 – 579	73	Cytoplasmic Potential
	Transmembrane	580 – 600	21	Helical; Potential
	Topological domain	601 – 642	42	Lumenal Potential
	Transmembrane	643 – 663	21	Helical; Potential
	Topological domain	664 – 813	150	Cytoplasmic Potential
	Repeat	133 – 162	30	ANK 1
	Repeat	167 – 196	30	ANK 2
	Repeat	201 – 231	31	ANK 3
	Repeat	235 – 264	30	ANK 4
	Repeat	276 – 305	30	ANK 5
	Repeat	309 – 338	30	ANK 6
	Zinc finger	536 – 586	51	DHHC-type
Sites
	Active site	566	1	S-palmitoyl cysteine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5AL27 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 6A27A94506E7AA90	FASTA	813	91,741
	10 20 30 40 50 60 MAKKKSKSKS SSPKPKVSST AAKPVEIDNQ QNIENVQDSP SALQQQSATA EESENTATTA 70 80 90 100 110 120 TPSEGTTATT ESSPVTTSNE TDPIELSVLD NADHAIDSNS IKSTEAVLET LDSELNTIAD 130 140 150 160 170 180 TKTEPPTEQE LNPTLAKYMR SCQEGNLTIV KELISSGQVL VNDTFSDEIT GLHWACINNR 190 200 210 220 230 240 LSLVKFLIAN GANPNQLGGE LKASPLHWAC RNGLVYIVDY LMRNSDADPN LRDAQTYNAL 250 260 270 280 290 300 HLAVHSSNIM LVIYLLLSCC STDSVKKVYI DEPDGSNRTA LHWASYQNDI FTINALLRFG 310 320 330 340 350 360 ADVSKVDNSL FIPLHWAFMK GYKSVLKALV EAGSDIYFKN DQNKNSFDIA KDMNCSNTWE 370 380 390 400 410 420 KVLIETGRDP KNNWAPLKPW VSAKLGKIIT FLTPYFLLPL SFNVLSMGGD QGGFIIPKLI 430 440 450 460 470 480 LAIGILGGGI YLLNKLIISQ YIFDDKKLAK SPILAGVFSA TAFWSVLVWL YNILPTTFIH 490 500 510 520 530 540 NFFANVIMAI LIAIFTWSFF KAMFINPGFV PTPADNNVIL SQVAQLIELG KFDTDHFCVN 550 560 570 580 590 600 SFVRKPLRSR YSKHNKRLIA RFDHSCPWVY NDIGVRNHKI FITFVYSLNM AIFVFLYLSL 610 620 630 640 650 660 QYFDKVKDQY DSDDEGEGEG FVCSILGDDM CYGYKNHHFH FNVFMWDLFQ CVWVSFLCIV 670 680 690 700 710 720 QTFQILKGLT TWEFSSLNKQ LQTNRFNHST VPRDFEAGEG ISLDQTQPSD GQHRHNHNEF 730 740 750 760 770 780 QTCMNLLGID QFILTLKMSL KSLFSNTRHG NNNTNYDPLT SLHIPTDHGI KQNWLDFWII 790 800 810 GEEKWRNVFY LPIDGENNLN GKVVDYYTLY SYH

References

[1]

"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACQ01000010 Genomic DNA. Translation: EAL03387.1. AACQ01000009 Genomic DNA. Translation: EAL03508.1.
RefSeq	XP_722161.1. XM_717068.1. XP_722273.1. XM_717180.1.
3D structure databases
ProteinModelPortal	Q5AL27.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5AL27.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3636062. 3636166.
KEGG	cal:CaO19.12414. cal:CaO19.4950.
Organism-specific databases
CGD	CAL0006326. AKR1.
Phylogenomic databases
PhylomeDB	Q5AL27.
Family and domain databases
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. IPR001594. Znf_DHHC_palmitoyltrfase. [Graphical view]
Gene3D	G3DSA:1.25.40.20. ANK. 1 hit.
KO	K06867.
Pfam	PF00023. Ank. 1 hit. PF12796. Ank_2. 1 hit. PF01529. zf-DHHC. 1 hit. [Graphical view]
SMART	SM00248. ANK. 6 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 3 hits. PS50216. ZF_DHHC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AKR1_CANAL

Accession

Primary (citable) accession number: Q5AL27

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	April 26, 2005
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program