Q5AGV7 (PFA4_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Palmitoyltransferase PFA4
EC=2.3.1.-

Alternative name(s):
Protein fatty acyltransferase 4

Gene names

Name:	PFA4
ORF Names:	CaJ7.0163, CaO19.13934, CaO19.6581

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Palmitoyl-CoA + protein-cysteine = S-palmitoyl protein + CoA.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Domain	The DHHC domain is required for palmitoyltransferase activity By similarity.
Post-translational modification	Autopalmitoylated By similarity.
Sequence similarities	Belongs to the DHHC palmitoyltransferase family. PFA4 subfamily. Contains 1 DHHC-type zinc finger.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Acyltransferase Transferase
PTM	Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	transferase activity, transferring acyl groups Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	Palmitoyltransferase PFA4		PRO_0000212962
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 29	21	Helical; Potential
	Topological domain	30 – 40	11	Lumenal Potential
	Transmembrane	41 – 61	21	Helical; Potential
	Topological domain	62 – 161	100	Cytoplasmic Potential
	Transmembrane	162 – 182	21	Helical; Potential
	Topological domain	183 – 201	19	Lumenal Potential
	Transmembrane	202 – 222	21	Helical; Potential
	Topological domain	223 – 446	224	Cytoplasmic Potential
	Zinc finger	114 – 164	51	DHHC-type

Sequences

	Sequence		Length	Mass (Da)
	Q5AGV7 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 3081431C4134E7F5	FASTA	446	52,757
	10 20 30 40 50 60 MAVQLKWPIL GVIIPCIIIF SLSYGSHYFI LRHHLTMKQQ LIYEFYVTMI WISYLLAIYT 70 80 90 100 110 120 NPGRVPKNYK PSLASSTRIE QTEDDSDGLG LESREDETLI REEPISGDRC EWIRYCKKCN 130 140 150 160 170 180 NYKPPRSHHC KICQQCVLQM DHHCPWTLNC VGNNNLPHFM RFLGWIIWGT GYLMIQLIKL 190 200 210 220 230 240 IINYYENSNM PHYLFNKTEL VAIIAITPLN FFVFASILVL FIRCLINICK GMTQIEIWEW 250 260 270 280 290 300 ERLELQWSSK RLWRLIRFNY GRLHKGKPFP ELNTWTNTTN NVNYNDNDDD GDEDVELINL 310 320 330 340 350 360 ATNNNEDSTI VPQNFTIDDL IFPYNLGIWK NLVNALGYPY MWLIPFGKPK SNGYQPQISQ 370 380 390 400 410 420 DYKQDDQLNL PWPPDGIRQK EIEINVLQQQ GYQRDREEED EEELRSIRNY QELRRRLDPR 430 440 LNVQRSDFIN DMGEGLTDFG VDEDSD

References

[1]	"Sequence finishing and gene mapping for Candida albicans chromosome 7 and syntenic analysis against the Saccharomyces cerevisiae genome." Chibana H., Oka N., Nakayama H., Aoyama T., Magee B.B., Magee P.T., Mikami Y. Genetics 170:1525-1537(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.
[2]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006852 Genomic DNA. Translation: BAE44668.1. AACQ01000016 Genomic DNA. Translation: EAL02617.1. AACQ01000020 Genomic DNA. Translation: EAL02083.1.
RefSeq	XP_720904.1. XM_715811.1. XP_721417.1. XM_716324.1. XP_888771.1. XM_883678.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0003300.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3636956. 3637541. 3704238.
KEGG	cal:CaO19.13934. cal:CaO19.6581. cal:CaO19_6581.
Organism-specific databases
CGD	CAL0003300. orf19.6581.
Phylogenomic databases
eggNOG	COG5273.
Family and domain databases
InterPro	IPR001594. Znf_DHHC_palmitoyltrfase. [Graphical view]
Pfam	PF01529. zf-DHHC. 1 hit. [Graphical view]
PROSITE	PS50216. ZF_DHHC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PFA4_CANAL

Accession

Primary (citable) accession number: Q5AGV7
Secondary accession number(s): Q3MPJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	April 26, 2005
Last modified:	April 3, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program