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Protein

tRNA(His) guanylyltransferase

Gene

THG1

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage.UniRule annotation

Catalytic activityi

p-tRNA(His) + ATP + GTP = pppG-P-tRNA(His) + AMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101ATPCombined sources
Binding sitei10 – 101GTPCombined sources
Metal bindingi29 – 291MagnesiumCombined sources
Metal bindingi30 – 301MagnesiumCombined sources
Binding sitei43 – 431GTP; via carbonyl oxygenCombined sources
Binding sitei47 – 471GTP; via amide nitrogenCombined sources
Metal bindingi76 – 761MagnesiumCombined sources
Binding sitei76 – 761ATPCombined sources
Binding sitei76 – 761GTPCombined sources
Binding sitei132 – 1321ATPCombined sources
Binding sitei132 – 1321GTPCombined sources
Binding sitei152 – 1521ATPCombined sources

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi27 – 348ATPCombined sources
Nucleotide bindingi27 – 348GTPCombined sources
Nucleotide bindingi44 – 474ATPCombined sources
Nucleotide bindingi92 – 954ATPCombined sources
Nucleotide bindingi92 – 954GTPCombined sources

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. GTP binding Source: UniProtKB-KW
  3. magnesium ion binding Source: InterPro
  4. tRNA guanylyltransferase activity Source: InterPro

GO - Biological processi

  1. tRNA modification Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

NucleotidyltransferaseUniRule annotationImported, Transferase

Keywords - Biological processi

tRNA processingUniRule annotation

Keywords - Ligandi

ATP-bindingCombined sources, GTP-bindingUniRule annotationCombined sources, MagnesiumCombined sources, Metal-bindingCombined sources, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA(His) guanylyltransferaseUniRule annotation (EC:2.7.7.79UniRule annotation)
Alternative name(s):
tRNA-histidine guanylyltransferaseUniRule annotation
Gene namesi
Name:THG1Imported
ORF Names:CaO19.7063Imported, I503_05065Imported, orf19.7063Imported, W5Q_05109Imported
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)Imported
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
ProteomesiUP000000559 Componenti: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0002111. orf19.7063.

Interactioni

Protein-protein interaction databases

STRINGi5476.CAL0002111.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3WBZX-ray2.39A/B/C/D/E/F/G/H1-268[»]
3WC0X-ray3.03A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P1-268[»]
3WC1X-ray4.18A/B/C/D1-262[»]
3WC2X-ray3.64A/B/C/D1-268[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA(His) guanylyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG4021.
InParanoidiQ5AFK5.
KOiK10761.
OrthoDBiEOG7QVMD6.

Family and domain databases

InterProiIPR025845. Thg1_C_dom.
IPR024956. tRNAHis_GuaTrfase_cat.
IPR007537. tRNAHis_GuaTrfase_Thg1.
[Graphical view]
PANTHERiPTHR12729. PTHR12729. 1 hit.
PfamiPF04446. Thg1. 1 hit.
PF14413. Thg1C. 1 hit.
[Graphical view]
PIRSFiPIRSF028980. tRNAHis_guanylyltransferase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5AFK5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANSKYEYVK LFEKENYLLP DTYIIIRVDG KGFHKFSQFY EFEKPNDLKA
60 70 80 90 100
LQVMNSAAEK LMSKYSDVML AYGDSDEYSF LLRKNCQLYE RREMKLTTLF
110 120 130 140 150
SSLMSTYYMY FWSQYFPDKP LHIDHLPNFD ARAVLYPDFK HIRNYFSWRQ
160 170 180 190 200
VDCHINNLYN TTFWNLVLKL KMTPQQAEQR LMGTVASDKN EILFKECGVN
210 220 230 240 250
YNNESEMYKK GTIIVREFEN YETEDEAELS KRQVQRLEKK RKKAELKIYH
260
VDIINDDSWW KSRPWLKD
Length:268
Mass (Da):32,442
Last modified:April 26, 2005 - v1
Checksum:i0BC7B911C05C49EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000024 Genomic DNA. Translation: EAL01510.1.
AJJF01000032 Genomic DNA. Translation: KHC71750.1.
JTBX01000063 Genomic DNA. Translation: KHC80871.1.
RefSeqiXP_720355.1. XM_715262.1.
XP_888688.1. XM_883595.1.

Genome annotation databases

GeneIDi3637997.
3704204.
KEGGical:CaO19.7063.
cal:CaO19_7063.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000024 Genomic DNA. Translation: EAL01510.1.
AJJF01000032 Genomic DNA. Translation: KHC71750.1.
JTBX01000063 Genomic DNA. Translation: KHC80871.1.
RefSeqiXP_720355.1. XM_715262.1.
XP_888688.1. XM_883595.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3WBZX-ray2.39A/B/C/D/E/F/G/H1-268[»]
3WC0X-ray3.03A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P1-268[»]
3WC1X-ray4.18A/B/C/D1-262[»]
3WC2X-ray3.64A/B/C/D1-268[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5476.CAL0002111.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3637997.
3704204.
KEGGical:CaO19.7063.
cal:CaO19_7063.

Organism-specific databases

CGDiCAL0002111. orf19.7063.

Phylogenomic databases

eggNOGiCOG4021.
InParanoidiQ5AFK5.
KOiK10761.
OrthoDBiEOG7QVMD6.

Family and domain databases

InterProiIPR025845. Thg1_C_dom.
IPR024956. tRNAHis_GuaTrfase_cat.
IPR007537. tRNAHis_GuaTrfase_Thg1.
[Graphical view]
PANTHERiPTHR12729. PTHR12729. 1 hit.
PfamiPF04446. Thg1. 1 hit.
PF14413. Thg1C. 1 hit.
[Graphical view]
PIRSFiPIRSF028980. tRNAHis_guanylyltransferase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SC5314Imported and SC5314 / ATCC MYA-2876Imported.
  2. "Structural basis of reverse nucleotide polymerization."
    Nakamura A., Nemoto T., Heinemann I.U., Yamashita K., Sonoda T., Komoda K., Tanaka I., Soll D., Yao M.
    Proc. Natl. Acad. Sci. U.S.A. 110:20970-20975(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.39 ANGSTROMS) IN COMPLEX WITH ATP; GTP AND MAGNESIUM.
  3. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: SC5314Imported.

Entry informationi

Entry nameiQ5AFK5_CANAL
AccessioniPrimary (citable) accession number: Q5AFK5
Secondary accession number(s): Q3MPS5
Entry historyi
Integrated into UniProtKB/TrEMBL: April 26, 2005
Last sequence update: April 26, 2005
Last modified: April 29, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.