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Protein

Glyoxalase 3

Gene

GLX3

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of methylglyoxal (MG) to D-lactate in a single glutathione (GSH)-independent step. Selective for MG, does not use glyoxal as substrate. Plays a role in detoxifying endogenously produced MG, particularly when glycerol is the principal carbon source (PubMed:24302734). Important for viability in stationary phase (By similarity).By similarity1 Publication

Catalytic activityi

(R)-lactate = methylglyoxal + H2O.1 Publication

Kineticsi

kcat is 7.8 sec(-1) with methylglyoxal as substrate.1 Publication
  1. KM=5.5 mM for methylglyoxal1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei1361 Publication1
    Active sitei1371 Publication1
    Active sitei1681 Publication1

    GO - Molecular functioni

    Keywordsi

    Molecular functionLyase
    Biological processStress response

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glyoxalase 31 Publication (EC:4.2.1.1301 Publication)
    Alternative name(s):
    Glutathione-independent glyoxalase1 Publication
    Gene namesi
    Name:GLX31 Publication
    Ordered Locus Names:CAALFM_C302610CA
    ORF Names:CaO19.251, CaO19.7882, orf19.251, orf19.7882
    OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
    Taxonomic identifieri237561 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
    Proteomesi
    • UP000000559 Componenti: Chromosome 3

    Organism-specific databases

    CGDiCAL0000194072. GLX3.

    Pathology & Biotechi

    Disruption phenotypei

    Has a 3- to 5-fold increase in levels of intracellular methylglyoxal compared with wild-type cells grown in the same media.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi136C → S: Abolishes catalytic activity. 1 Publication1
    Mutagenesisi137H → F: Reduces catalytic activity by 75%. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004321101 – 236Glyoxalase 3Add BLAST236

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei136Cysteine sulfinic acid (-SO2H)By similarity1

    Keywords - PTMi

    Oxidation

    Proteomic databases

    PRIDEiQ5AF03.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Structurei

    Secondary structure

    1236
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi2 – 7Combined sources6
    Helixi26 – 39Combined sources14
    Beta strandi42 – 50Combined sources9
    Helixi56 – 58Combined sources3
    Turni61 – 63Combined sources3
    Helixi66 – 73Combined sources8
    Helixi78 – 84Combined sources7
    Helixi89 – 91Combined sources3
    Helixi94 – 96Combined sources3
    Beta strandi98 – 102Combined sources5
    Helixi108 – 111Combined sources4
    Helixi112 – 114Combined sources3
    Helixi116 – 127Combined sources12
    Beta strandi131 – 135Combined sources5
    Helixi138 – 142Combined sources5
    Turni148 – 150Combined sources3
    Beta strandi151 – 153Combined sources3
    Turni154 – 157Combined sources4
    Helixi165 – 170Combined sources6
    Helixi174 – 179Combined sources6
    Helixi185 – 192Combined sources8
    Beta strandi207 – 210Combined sources4
    Beta strandi213 – 216Combined sources4
    Helixi219 – 221Combined sources3
    Helixi222 – 233Combined sources12

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4LRUX-ray1.60A1-236[»]
    ProteinModelPortaliQ5AF03.
    SMRiQ5AF03.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    InParanoidiQ5AF03.
    OrthoDBiEOG092C4O4J.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    InterProiView protein in InterPro
    IPR029062. Class_I_gatase-like.
    IPR002818. DJ-1/PfpI.
    PfamiView protein in Pfam
    PF01965. DJ-1_PfpI. 1 hit.
    SUPFAMiSSF52317. SSF52317. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5AF03-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVKVLLALTS YNETFYSDGK KTGVFVVEAL HPFEVFRKKG YEIQLASETG
    60 70 80 90 100
    TFGWDDHSVV PDFLNGEDKE IFDNVNSEFN VALKNLKKAS DLDPNDYDIF
    110 120 130 140 150
    FGSAGHGTLF DYPNAKDLQK IATTVYDKGG VVSAVCHGPA IFENLNDPKT
    160 170 180 190 200
    GEPLIKGKKI TGFTDIGEDI LGVTDIMKKG NLLTIKQVAE KEGATYIEPE
    210 220 230
    GPWDNFTVTD GRIVTGVNPQ SAVKTAEDVI AAFECN
    Length:236
    Mass (Da):25,812
    Last modified:April 26, 2005 - v1
    Checksum:i4C77E841710C4542
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP017625 Genomic DNA. Translation: AOW28295.1.
    RefSeqiXP_719950.2. XM_714857.2.

    Genome annotation databases

    EnsemblFungiiEAL01090; EAL01090; CaO19.251.
    EAL01225; EAL01225; CaO19.7882.
    GeneIDi3638325.
    KEGGical:CAALFM_C302610CA.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    CP017625 Genomic DNA. Translation: AOW28295.1.
    RefSeqiXP_719950.2. XM_714857.2.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4LRUX-ray1.60A1-236[»]
    ProteinModelPortaliQ5AF03.
    SMRiQ5AF03.
    ModBaseiSearch...
    MobiDBiSearch...

    Proteomic databases

    PRIDEiQ5AF03.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiEAL01090; EAL01090; CaO19.251.
    EAL01225; EAL01225; CaO19.7882.
    GeneIDi3638325.
    KEGGical:CAALFM_C302610CA.

    Organism-specific databases

    CGDiCAL0000194072. GLX3.

    Phylogenomic databases

    InParanoidiQ5AF03.
    OrthoDBiEOG092C4O4J.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    InterProiView protein in InterPro
    IPR029062. Class_I_gatase-like.
    IPR002818. DJ-1/PfpI.
    PfamiView protein in Pfam
    PF01965. DJ-1_PfpI. 1 hit.
    SUPFAMiSSF52317. SSF52317. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiHSP31_CANAL
    AccessioniPrimary (citable) accession number: Q5AF03
    Secondary accession number(s): A0A1D8PJH1, Q5AEL7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 4, 2015
    Last sequence update: April 26, 2005
    Last modified: May 10, 2017
    This is version 66 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Candida albicans
      Candida albicans: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Peptidase families
      Classification of peptidase families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.