Q5ADX5 (MCES_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
mRNA cap guanine-N7 methyltransferase
EC=2.1.1.56

Alternative name(s):
mRNA (guanine-N(7)-)-methyltransferase
mRNA cap methyltransferase

Gene names

Name:	ABD1
Synonyms:	CCM1
ORF Names:	CaO19.6716, CaO19.14008

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for methylating the 5'-cap structure of mRNAs. Ref.1
Catalytic activity	S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. mRNA cap methyltransferase family.

Ontologies

Keywords
Biological process	mRNA capping mRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	cellular response to drug Inferred from mutant phenotype. Source: CGD mRNA capping Inferred from mutant phenotype. Source: CGD
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from direct assay. Source: CGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	mRNA cap guanine-N7 methyltransferase		PRO_0000303905
Experimental info
	Sequence conflict	86	1	V → A in BAA82446. Ref.2
	Sequence conflict	133	1	T → K in AAF69030. Ref.1
	Sequence conflict	295	1	I → V in BAA82446. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q5ADX5 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: EE195FE2695A1238	FASTA	474	54,248
	10 20 30 40 50 60 MSTDSYTPSQ EPGSKRLKTG ESVFARRGVS PSTGGVASAY GNESEKKPSW LQTNKSDIDG 70 80 90 100 110 120 KYDKYGERRN AHTTTRDSRL DRLKRVRQKS AEREDVGHEG DEGDEDEGIL PYIHLQAANP 130 140 150 160 170 180 AIIHNEKQEN YRTFQSRISN RENRDINSIV RAHYNQRTQQ AKQQGSRVNS PIYKMRNFNN 190 200 210 220 230 240 AIKYILLGNW AKHNPEELDL FSFLDLCCGK GGDLNKCQFI GIDQYIGIDI ADLSVKEAFE 250 260 270 280 290 300 RYTKQKARFR HSNQNSNRYT FEACFATGDC FTQFVPDILE PNFPGIIERA FPVDIVSAQF 310 320 330 340 350 360 SLHYSFESEE KVRTLLTNVT RSLRSGGTFI GTIPSSDFIK AKIVDKHLQR DEKGKAKFGN 370 380 390 400 410 420 SLYSVTFEKD PPEDGVFRPA FGNKYNYWLK DAVDNVPEYV VPFETLRSLC EEYDLVLKYK 430 440 450 460 470 KSFTDIFNQE IPKYFSKLNK NLIDGMKRSD GKYGAEGDEK EAVAFYIGFV FEKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of human, Schizosaccharomyces pombe, and Candida albicans mRNA cap methyltransferases and complete replacement of the yeast capping apparatus by mammalian enzymes." Saha N., Schwer B., Shuman S. J. Biol. Chem. 274:16553-16562(1999) [PubMed: 10347220] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF133529 Genomic DNA. Translation: AAF69030.1. AB020965 Genomic DNA. Translation: BAA82446.1. AACQ01000029 Genomic DNA. Translation: EAL00823.1. AACQ01000028 Genomic DNA. Translation: EAL00948.1.
RefSeq	XP_719698.1. XM_714605.1. XP_719815.1. XM_714722.1.
3D structure databases
HSSP	HSSP built from PDB template 1RI5 based on UniProtKB Q8SR66.
ProteinModelPortal	Q5ADX5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5ADX5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3638456. 3638594.
KEGG	cal:CaO19.14008. cal:CaO19.6716.
Organism-specific databases
CGD	CAL0004754. ABD1.
Phylogenomic databases
PhylomeDB	Q5ADX5.
Family and domain databases
InterPro	IPR016899. mRNA_G-N7_MeTrfase. IPR004971. Pox_MCEL. [Graphical view]
KO	K00565.
Pfam	PF03291. Pox_MCEL. 1 hit. [Graphical view]
PIRSF	PIRSF028762. ABD1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCES_CANAL

Accession

Primary (citable) accession number: Q5ADX5
Secondary accession number(s): Q9P936, Q9Y8H4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	April 26, 2005
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program