Q5AD67 (CHL1_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent RNA helicase CHL1
EC=3.6.4.13

Alternative name(s):
Chromosome loss protein 1

Gene names

Name:	CHL1
ORF Names:	CaO19.2000, CaO19.9551

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	842 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Important for chromosome transmission and normal cell cycle progression in G2/M. May have a role in changing DNA topology to allow the loading of proteins involved in maintaining sister chromatid cohesion in the vicinity of the centromeres. Has a specific role in chromosome segregation during meiosis II By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the DEAD box helicase family. DEAH subfamily. DDX11/CHL1 sub-subfamily. Contains 1 helicase ATP-binding domain.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent DNA helicase activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 842	842	ATP-dependent RNA helicase CHL1		PRO_0000351006
Regions
	Domain	6 – 417	412	Helicase ATP-binding
	Nucleotide binding	42 – 49	8	ATP By similarity
	Motif	359 – 362	4	DEAH box
	Compositional bias	492 – 499	8	Poly-Ser
	Compositional bias	821 – 826	6	Poly-Asn

Sequences

	Sequence		Length	Mass (Da)
	Q5AD67 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: A241696BAC817F99	FASTA	842	97,275
	10 20 30 40 50 60 MVSESCSRNY NHPYTPYDIQ IQLMDAIYNT IENGYKIGLF ESPTGTGKTL SIICSSMTWL 70 80 90 100 110 120 RTFKRNNTFL ETNNEVEDVY ESESEEDEPE WVKKAYQSSI VNRSKNKLIE YEHYLDKIEK 130 140 150 160 170 180 EHAQNKRKEE ELEIKVHKRR KAMTAAGTDL SEESYLPMDY YSDSEVGKIE DQNLAITKEI 190 200 210 220 230 240 NRLLKKVENK EEVSYINECP IKIFFSSRTH SQLNQFSSQL RLTNFQASFE DLEERTKYIP 250 260 270 280 290 300 LGSRKQLCIN EKVRSKGNDQ SVNDACLDLQ RETNGCQYLP KNYMMSSVTK EFADLSLAKI 310 320 330 340 350 360 RDIEDLNELG IELNICPYYS VRKGIEMTEI ISLPYQMIFQ DTTRKILNLD IKDSIIIIDE 370 380 390 400 410 420 AHNIIDVITS MYSIKITSDQ LNKVIKSLKI YLNKFLKRLN SGNRINLMKL IKICQILLKF 430 440 450 460 470 480 LNTNSEKVKS GDEVQIQDIF KDSTGDLVNI HKLDQFLTKS KIAYKIESYI EKTEMETDNG 490 500 510 520 530 540 EKKGRITNSG GSSSSSSSSN PLLFTIIKFL RTLTNLSKEG KFFWDNENGT ISLNYMLLDP 550 560 570 580 590 600 SAVFKEIVDQ AKCVLLCGGT MEPMSDYMDY LFPSVPTNKI NTFACGHVIP KENLQVFPIS 610 620 630 640 650 660 QWNDTNFEFS YQKRNDSKQL MALGEFLIEI TKRVPYGVVI FFPSYKYLDQ VLQFWRDTKI 670 680 690 700 710 720 LTSIESEKTI FREPKDPSNV EKVLNEYGYL IQTERKGAIL FSVVGGKMSE GINFSDDLAR 730 740 750 760 770 780 AVIMVGLPYP NAYSGEMVTK RKYIETSELS NGGTTTDAKE KSRNYYENLC MRAVNQSIGR 790 800 810 820 830 840 SIRHINDYSI IYLVDRRFST PRIQNKLSQW VKERISITTT NNNNNNSIYI MESTTDFFNI IR

References

[1]

"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACQ01000031 Genomic DNA. Translation: EAL00566.1. AACQ01000030 Genomic DNA. Translation: EAL00698.1.
RefSeq	XP_719450.1. XM_714357.1. XP_719578.1. XM_714485.1.
3D structure databases
ProteinModelPortal	Q5AD67.
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0004313.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3638692. 3638794.
KEGG	cal:CaO19.2000. cal:CaO19.9551.
Phylogenomic databases
eggNOG	COG1199.
KO	K11273.
Family and domain databases
InterPro	IPR006555. ATP-dep_Helicase_C. IPR027076. DDX11/CHL1. IPR010614. DEAD_2. IPR002464. DNA/RNA_helicase_DEAH_CS. IPR013020. DNA_helicase_DNA-repair_Rad3. IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3. IPR006554. Helicase-like_DEXD_c2. [Graphical view]
PANTHER	PTHR11472:SF5. PTHR11472:SF5. 1 hit.
Pfam	PF06733. DEAD_2. 1 hit. PF13307. Helicase_C_2. 1 hit. [Graphical view]
SMART	SM00488. DEXDc2. 1 hit. SM00491. HELICc2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00604. rad3. 1 hit.
PROSITE	PS00690. DEAH_ATP_HELICASE. 1 hit. PS51193. HELICASE_ATP_BIND_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHL1_CANAL

Accession

Primary (citable) accession number: Q5AD67

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	April 26, 2005
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program