Q5AAW3 (DHH1_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent RNA helicase DHH1
EC=3.6.4.13

Gene names

Name:	DHH1
ORF Names:	CaO19.13577, CaO19.6197

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	549 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	ATP-dependent RNA helicase involved in mRNA turnover, and more specifically in mRNA decapping. Is involved in G1/S DNA-damage checkpoint recovery, probably through the regulation of the translational status of a subset of mRNAs. May also have a role in translation and mRNA nuclear export By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subcellular location	Cytoplasm › P-body By similarity. Note: Is concentrated in several cytoplasmic foci called P bodies (or cytoplasmic processing bodies) which represent sites of mRNA decapping and 5' to 3' exonucleotidic decay By similarity.
Domain	The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.
Sequence similarities	Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Translation regulation Transport mRNA processing mRNA transport
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW mRNA transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasmic mRNA processing body Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasmic stress granule Inferred from direct assay PubMed 22056521. Source: CGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 549	549	ATP-dependent RNA helicase DHH1		PRO_0000232186
Regions
	Domain	61 – 231	171	Helicase ATP-binding
	Domain	241 – 401	161	Helicase C-terminal
	Nucleotide binding	74 – 81	8	ATP By similarity
	Motif	30 – 58	29	Q motif
	Motif	179 – 182	4	DEAD box
	Compositional bias	448 – 548	101	Gln-rich

Sequences

	Sequence		Length	Mass (Da)
	Q5AAW3 [UniParc]. Last modified April 26, 2005. Version 1. Checksum: 1AA169EF435AA0BF	FASTA	549	62,110
	10 20 30 40 50 60 MTDTNWKQNL NLPPKDTRPQ TEDVLNTKGK SFEDFNLKRE LLMGIFEAGF EKPSPIQEES 70 80 90 100 110 120 IPMALAGRDI LARAKNGTGK TASFIIPCLQ LVKPKLNKVQ ALILVPTREL ALQTSQVVRT 130 140 150 160 170 180 LGKHVGTQCM VTTGGTSLRD DIVRLHDPVH ILVGTPGRVL DLAARKVVDL SECPLFVMDE 190 200 210 220 230 240 ADKMLSREFK GIIEQILEFF PPNRQALLFS ATFPLAVKSF MDKHLTKPYE INLMDELTLK 250 260 270 280 290 300 GISQFYAFVE EKQKLHCLNT LFSKLQINQS IIFCNSTNRV ELLAKKITEL GYSCYYSHAK 310 320 330 340 350 360 MPQQARNKVF HEFRQGKVRN LVCSDLLTRG IDIQAVNVVI NFDFPKTAET YLHRIGRSGR 370 380 390 400 410 420 FGHLGLAINL MSWNDRYSLY KIEQELGTEI KPIPATIDKS LYVAENADAV PRPFRIDELP 430 440 450 460 470 480 KGNETVHNKG YQYKGQPVKD ENSGSSSQQQ QQPPPQQQQQ QSTSPPQQQP QQQQQQPNPQ 490 500 510 520 530 540 HQFAPHPNGQ FPPYPQQFHQ PGAIPPQQFN GYPPYPQYPP QFAGYPGQPP QLPQGQQQHA QAQNPAQQY

References

[1]

"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACQ01000037 Genomic DNA. Translation: EAK99880.1. AACQ01000038 Genomic DNA. Translation: EAK99792.1.
RefSeq	XP_718704.1. XM_713611.1. XP_718788.1. XM_713695.1.
3D structure databases
ProteinModelPortal	Q5AAW3.
SMR	Q5AAW3. Positions 31-406.
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0006152.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3639490. 3639628.
KEGG	cal:CaO19.13577. cal:CaO19.6197.
Organism-specific databases
CGD	CAL0006152. orf19.6197.
Phylogenomic databases
eggNOG	COG0513.
KO	K12614.
Family and domain databases
InterPro	IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR000629. RNA-helicase_DEAD-box_CS. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00039. DEAD_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHH1_CANAL

Accession

Primary (citable) accession number: Q5AAW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	April 26, 2005
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program