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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

TRM8

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei122S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei219S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei222UniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Transfer RNA methyltransferase 8UniRule annotation
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:TRM8UniRule annotation
Ordered Locus Names:CAALFM_C404810CA
ORF Names:CaO19.11279, CaO19.3798
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
Proteomesi
  • UP000000559 Componenti: Chromosome 4

Organism-specific databases

CGDiCAL0000183898. orf19.3798.

Subcellular locationi

  • Nucleus UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003705911 – 325tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST325

Proteomic databases

PRIDEiQ5A692.

Interactioni

Subunit structurei

Forms a complex with TRM82.UniRule annotation

Structurei

3D structure databases

SMRiQ5A692.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni145 – 146S-adenosyl-L-methionine bindingUniRule annotation2
Regioni199 – 200S-adenosyl-L-methionine bindingUniRule annotation2
Regioni297 – 299S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

InParanoidiQ5A692.
KOiK03439.
OrthoDBiEOG092C0GMH.

Family and domain databases

HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PTHR23417:SF20. PTHR23417:SF20. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 2 hits.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5A692-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEATDKQKQ VSAETPLERD LKSKQQSIAA TKRKQYRDDK SQIRKQARFE
60 70 80 90 100
VSTTPEPEQS DSSATTATIT ELPKKRYYRQ RAHSNPFSDH RLEYPKSPES
110 120 130 140 150
MDWSNLYPKQ YDISKVEIAD IGCGYGGLMI KLGPQFPKSL ILGLEIRVQV
160 170 180 190 200
TQYVEDRIIA LRKNQEIIND KKKKESGGGT GDGDDNDYSY QNIAVLRGNA
210 220 230 240 250
MKFLPNFFVK GQLSKMFFCF PDPHFKQRKH KARIITNTLL SEYAYVLREG
260 270 280 290 300
GVVYTITDVE DLHNWMVKHL DEHPLFERLS KEWEDQDKCV EIMYNATEEG
310 320
QKVTRNKGSK WVACYKRLPS PDDCE
Length:325
Mass (Da):37,595
Last modified:April 12, 2017 - v3
Checksum:i58441953C685AC99
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP017626 Genomic DNA. Translation: AOW29224.1.
RefSeqiXP_717203.2. XM_712110.2.

Genome annotation databases

GeneIDi3641112.
KEGGical:CAALFM_C404810CA.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTRMB_CANAL
AccessioniPrimary (citable) accession number: Q5A692
Secondary accession number(s): A0A1D8PM52
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: April 12, 2017
Last modified: July 5, 2017
This is version 80 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families