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Q5A692 (TRMB_CANAL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:TRM8
ORF Names:CaO19.11279, CaO19.3798
OrganismCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]
Taxonomic identifier237561 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesmitosporic SaccharomycetalesCandida

Protein attributes

Sequence length325 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subunit structure

Forms a complex with TRM82 By similarity. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Sequence caution

The sequence EAK98229.1 differs from that shown. Reason: Erroneous initiation.

The sequence EAK98306.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 325325tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370591

Regions

Region145 – 1462S-adenosyl-L-methionine binding By similarity
Region199 – 2002S-adenosyl-L-methionine binding By similarity
Region297 – 2993S-adenosyl-L-methionine binding By similarity

Sites

Active site2221 By similarity
Binding site1221S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site2191S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5A692 [UniParc].

Last modified May 5, 2009. Version 2.
Checksum: AF55D2784BB9FAA9

FASTA32537,537
        10         20         30         40         50         60 
MSEATDKQEQ VSAETPLERD LKSKQQSIAA TKRKQYRDDK SQIRKQARFE VSTTPEPEQS 

        70         80         90        100        110        120 
DSSATTATIT ELPKKRYYRQ RAHSNPFSDH RLEYPKSPES MDWSNLYPKQ YDISKVEIAD 

       130        140        150        160        170        180 
IGCGYGGLMI KLGPQFPKSL ILGLEIRVQV TQYVEDRIIA LRKNQEIING KKKKESGGGT 

       190        200        210        220        230        240 
GDGDDNDYSY QNIAVLRGNA MKFLPNFFVK GQLSKMFFCF PDPHFKQRKH KARIITNTLL 

       250        260        270        280        290        300 
SEYAYVLREG GVVYTITDVE DLHNWMVKHL DEHPLFERLS KEWEDQDKCV EIMYNATEEG 

       310        320 
QKVTRNKGSK WVACYKRLPS PDDCE 

« Hide

References

[1]"The diploid genome sequence of Candida albicans."
Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S.
Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AACQ01000057 Genomic DNA. Translation: EAK98229.1. Different initiation.
AACQ01000056 Genomic DNA. Translation: EAK98306.1. Different initiation.
RefSeqXP_717203.1. XM_712110.1.
XP_717279.1. XM_712186.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5476.CAL0001314.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3641083.
3641112.
KEGGcal:CaO19.11279.
cal:CaO19.3798.

Organism-specific databases

CGDCAL0001314. orf19.3798.

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OrthoDBEOG708W9T.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 2 hits.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_CANAL
AccessionPrimary (citable) accession number: Q5A692
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: June 11, 2014
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Candida albicans

Candida albicans: entries and gene names