Q5A692 (TRMB_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
Transfer RNA methyltransferase 8
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	TRM8
ORF Names:	CaO19.11279, CaO19.3798

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast) [Reference proteome]

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida albicans

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA.
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Subunit structure	Forms a complex with TRM82 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.
Sequence caution	The sequence EAK98229.1 differs from that shown. Reason: Erroneous initiation. The sequence EAK98306.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	tRNA (guanine-N(7)-)-methyltransferase		PRO_0000370591
Regions
	Region	145 – 146	2	S-adenosyl-L-methionine binding By similarity
	Region	199 – 200	2	S-adenosyl-L-methionine binding By similarity
	Region	297 – 299	3	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	222	1	By similarity
	Binding site	122	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	219	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5A692 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: AF55D2784BB9FAA9	FASTA	325	37,537
	10 20 30 40 50 60 MSEATDKQEQ VSAETPLERD LKSKQQSIAA TKRKQYRDDK SQIRKQARFE VSTTPEPEQS 70 80 90 100 110 120 DSSATTATIT ELPKKRYYRQ RAHSNPFSDH RLEYPKSPES MDWSNLYPKQ YDISKVEIAD 130 140 150 160 170 180 IGCGYGGLMI KLGPQFPKSL ILGLEIRVQV TQYVEDRIIA LRKNQEIING KKKKESGGGT 190 200 210 220 230 240 GDGDDNDYSY QNIAVLRGNA MKFLPNFFVK GQLSKMFFCF PDPHFKQRKH KARIITNTLL 250 260 270 280 290 300 SEYAYVLREG GVVYTITDVE DLHNWMVKHL DEHPLFERLS KEWEDQDKCV EIMYNATEEG 310 320 QKVTRNKGSK WVACYKRLPS PDDCE

References

[1]

"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACQ01000057 Genomic DNA. Translation: EAK98229.1. Different initiation. AACQ01000056 Genomic DNA. Translation: EAK98306.1. Different initiation.
RefSeq	XP_717203.1. XM_712110.1. XP_717279.1. XM_712186.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	5476.CAL0001314.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3641083. 3641112.
KEGG	cal:CaO19.11279. cal:CaO19.3798.
Organism-specific databases
CGD	CAL0001314. orf19.3798.
Phylogenomic databases
eggNOG	COG0220.
KO	K03439.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. IPR025763. tRNA_(Gua-N-7)_MeTrfase_euk. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_CANAL

Accession

Primary (citable) accession number: Q5A692

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 5, 2009
Last modified:	April 3, 2013
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program